H Isaka

National Institute of Health Sciences

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Erosive pustular dermatosis of the scalp successfully treated with oral zinc sulphate

British Journal of Dermatology (1982)

Mitsunori Ikeda J Arata H Isaka

10.1111/j.1365-2133.1982.tb14715.x

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Citations (17)

Sodium deoxycholate-polyacrylamide gel electrophoresis of lipopolysaccharides at low temperature.

Chemical and Pharmaceutical Bulletin (1988)

Tetsuo Komuro Chikako Yomota H Isaka

Sodium deoxycholate (DOC)-polyacrylamide gel electrophoresis (PAGE) has previously been used to examine in detail the chemical heterogeneity of lipopolysaccharide (LPS) : It is now shown that DOC-PAGE carried out at 4°C can give reproducible banding patterns even when a large amount of LPS (20mg) is applied to the slab gel (4×150×300mm). By DOC-PAGE, we found that LPS obtained from E. coli UKT-B showed 14 silver-staining bands on gels but lacked the bands corresponding to a series of high-molecular-weight LPS usually observed in the S from LPS.These findings suggest that the DOC-PAGE at 4°C should be useful in fractionating LPS intactly to study LPS preparations with unknown structures.

Polyacrylamide

Silver stain

10.1248/cpb.36.1218

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Citations (5)

Spectrophotometric determination of sodium cyclamate by solvent extraction with Methylene Blue

BUNSEKI KAGAKU (1971)

Tadashi SHIBATA H Isaka Kiyoshi Yoshimura

シクラミン酸は塩基性色素のメチレンブルーと吸収極大660mμのクロロホルム可溶性の塩を形成する.これをシクラミン酸塩の定量に応用した.抽出の至適pHは7でシクラミン酸ナトリウム1～10μg/mlの濃度範囲で検量線は直線関係が得られた.シクラミン酸ナトリウムとメチレンブルー,および両者の塩はペーパークロマトグラフィーで異なるRf値を示し,塩形成がなされていることがわかった.また塩の組成は連続変化法および誘電率測定の結果から,クロロホルム溶液,水溶液ともにモル比が1:1であることがわかった.定量を妨害する共存物質を検討したところ,サッカリンは正誤差を与え,アミノ酸とたん白質は負誤差を与えるが,ソルビット,リンゴ酸などは5～1000倍モル存在しても妨害しないことを認めた.また製剤中のシクラミン酸をQAE-セファデックスを用いて分離したのち,この方法を用いて定量できることがわかった.

Methylene blue

Methylene

10.2116/bunsekikagaku.20.850

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Studies on Morphine Alkaloids. VII. Microbial Transformation of 14β-Bromocodeinone

Chemical and Pharmaceutical Bulletin (1970)

Kaoru Abe Masayuki Onda H Isaka Shigenobu Okuda

In the microbial transformation by Trametes sanguinea, 14β-bromocodeinone (IV) was first converted enzymatically into 14β-bromocodeine (VI), which was gradually affected chemically to form VII, II, and VIII as the final products.

Chemical transformation

10.1248/cpb.18.2070

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Citations (3)

Separation and identification of amine-carbohydrate reaction product in aqueous solution.

Chemical and Pharmaceutical Bulletin (1987)

Satoshi Okada Hiroshi Nakahara H Isaka Tooru Taga Koichiro Miyajima

Drugs with a primary aromatic amino moiety such as procaine, procainamide, and p-aminobenzoic acid (PABA) are capable of reacting with various reducing sugars containing hexoaldoses and pentoaldoses. The amine-carbohydrate reaction products can be separated from the parent drugs by high performance liquid chromatography (HPLC) analysis. The formation and dissociation profiles of the products were followed by HPLC and ultraviolet spectroscopy. The reaction products formed in aqueous solution were not association complexes, but chemical reaction products, which were identified as glycosylamines or N-glycosides, by comparison with authentic samples obtained by the condensation of amines with carbohydrates in methanol. An X-ray analysis of one of the glycosylamines, 2-deoxy-N-p-carboxyphenyl-D-ribosylamine, was carried out, and the absolute conformation and configuration in the crystalline state were determined.

Moiety

10.1248/cpb.35.2495

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Citations (2)

Intramolecular oxidative coupling of N-methylcoclaurine by a fungal enzyme system

Zeitschrift für allgemeine Mikrobiologie (1967)

Kyosuke Tsuda Hiroshi IIZUKA Shigenobu Okuda Mitsugi Iida H Isaka

10.1002/jobm.3630070311

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Coloration in the Porter-Silber Reaction of Betamethasone and Dexamethasone

YAKUGAKU ZASSHI (1976)

Tadashi SHIBATA H Isaka K. Mochida

The main Porter-Silber reaction products of Betamethasone (I) was 11β, 21-dihydroxy-9α-fluoro-16β-methyl-1, 4-pregnadiene-3, 20-dione (V), and 21-phenylhydrazone (III) of I was not found as the reaction product. On the contrary, the main product from Dexamethasone (II) was 21-phenylhydrazone (IV) of II. The reaction mixture of I, V, and II with PS reagent showed absorption maxima at 450, 450, and 423 nm, respectively. It was found that the chromophore showing an absorption maximum at 450 nm was derived from I or V by the reaction with sulfuric acid.

10.1248/yakushi1947.96.6_810

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[Morphine content of Japanese opium collected during 1959-1960].

PubMed (1961)

Y. NAKAGAWA H Isaka Youichi Miyazawa

Content (measure theory)

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[Studies on the potentiometric titrations of chinoform].

PubMed (1968)

H Isaka Masao Noguchi Tomohiro Shibata Kiyoshi Yoshimura

Amperometric titration

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Syntheses of Glucosides. II

YAKUGAKU ZASSHI (1953)

Kariyone Tatsuo Mitsuo Takahashi Kiyokazu Takaishi H Isaka

Pharmacological tests were carried out on 15 kinds of synthetic glucosides, including 11 new compounds, in the Pharmacological Laboratory of University of Kyoto. As a result, they were all found to have the action of slackening and paralyzing the muscles, although their actions were weaker than that of Myanesin. It was also found that Van't Hoff's Law of additive action of optical rotations could be applied to these glucosides and their acetylated products.

Glucoside

10.1248/yakushi1947.73.4_402

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Citations (5)