// Jianzhou Tang 1, 5, * , Hui Li 2, * , Jiashun Luo 3 , Hua Mei 4 , Liang Peng 1 , Xiaojie Li 5 1 Department of Biological and Environmental Engineering, Changsha University, Changsha 410003, Hunan, China 2 Department of Microbiology and Immunology, Medical School of Jishou University, Jishou 416000, Hunan, China 3 Institute of Medical Sciences, Medical School of Jishou University, Jishou 416000, Hunan, China 4 Hunan Guangxiu Hospital, Changsha 410002, Hunan, China 5 College of Animal Science and Technology of Hunan Agriculture University, Changsha 410128, Hunan, China * These authors contributed equally to this work and should be considered as co-first authors Correspondence to: Jianzhou Tang, email: Z20050711@ccsu.edu.cn Keywords: LSP1, breast cancer, risk, meta-analysis Received: June 06, 2016 Accepted: August 26, 2016 Published: August 31, 2016 ABSTRACT The association between the LSP1 rs3817198 T > C polymorphism and breast cancer risk has been widely investigated, but remains controversial. We therefore undertook a comprehensive meta-analysis to provide a high-quality evaluation of this association. A literature search was performed among Pubmed, EMBASE and Chinese National Knowledge Infrastructure (CNKI) databases prior to July 31, 2016, and the strength of the association between the LSP1 rs3817198 T > C polymorphism and breast cancer risk was assessed based on odds ratio (OR) and 95% confidence interval (95% CI). In total, 12 studies with 50,525 cases and 54,302 controls were included. Pooled risk estimates indicated a significant association between the LSP1 rs3817198 T > C polymorphism and breast cancer risk. Analysis of cases stratified based on ethnicity suggested that the association was significant in both Caucasian and Asian populations. Stratification based on source of controls revealed an association only in population-based studies. These findings indicate the LSP1 rs3817198 T > C polymorphism is associated with increased risk of breast cancer, especially in Caucasian and Asian populations. Large, well-designed studies with different ethnicities are still needed to verify our findings.
Objective To investigate the expression of cox-2 in 37 cases of osteosarcoma and 54 cases of giant cell tumor of bone,and analyze its significance in diagnosis and prognosis of osteosarcoma and giant cell tumor of bone.Methods lmmunohistochemical strain was used to detect the expression of cox-2 in 37 cases of osteosarcoma and 54 cases of giant cell tumor of bone.Results 29 cafes of giant cell tumor of bone were cox-2 positive,the expression of cox-2 was related to the recurrence.32 cases of osteosarcoma were cox-2 positive.The expression of cox-2 was related to the grade,but not related to the differentiation.Conclusion Cox-2 was related to the generation and prognosis,it may be an indicator for clinical diagnosis and prognosis evaluation of osteosarcoma and giant cell tumor of bone.
Key words:
Prostaglandin-endoperoxide synthase/ME; Osteosarcoma/ME; Giant cell tumor of bone/ME
MicroRNAs (miRs) are small non-coding RNAs that suppress gene expression by directly binding to the 3'-untranslated region of their target mRNAs. Specific miRs serve key roles in the development and progression of non-small cell lung cancer (NSCLC). The aim of the present study was to determine the mechanism of miR-17-5p in the regulation of NSCLC cell survival and proliferation. Reverse transcription-quantitative polymerase chain reaction data indicated that miR-17-5p was significantly downregulated in 28 NSCLC tissues compared with 7 non-tumorous lung tissues. Furthermore, lower miR-17-5p expression was associated with a higher pathological stage in NSCLC patients. Lower miR-17-5p expression was also observed in several common NSCLC cell lines, including SK-MES-1, A549, SPCA-1, H460, H1229 and HCC827, compared with the bronchial epithelium cell line, BEAS-2B. Additionally, overexpression of miR-17-5p significantly inhibited proliferation while inducing the apoptosis of NSCLC H460 cells. Subsequently, transforming growth factor β receptor 2 (TGFβR2) was identified as a direct target of miR-17-5p using a luciferase reporter assay. Western blot analysis confirmed that miR-17-5p negatively mediated the expression of TGFβR2 in NSCLC cells. Furthermore, small interfering RNA-induced downregulation of TGFβR2 also suppressed the proliferation of H460 cells while triggering apoptosis. Therefore, the results of the current study suggest that miR-17-5p may inhibit proliferation and trigger apoptosis in NSCLC H460 cells at least partially by targeting TGFβR2.
[Objective] This study aimed to investigate the mechanism of action of Eucommia ulmoides extract in treating atherosclerosis using network pharmacology and molecular docking technology. [Methods] The researchers screened the chemical active ingredients of Eucommia ulmoides using the TCMSP platform and collected the corresponding targets of these active ingredients using the Swiss Target Prediction platform. They searched for relevant targets of atherosclerosis in the DisGeNET, GeneCards, TTD, OMIM, Drug Bank, and Pharmgkb databases and obtained common targets between the two using the Venny platform. These common targets were then used to construct a protein interaction network and a core target network using Cytoscape 3.8.0, and topological parameters were obtained. GO and KEGG pathway enrichment analyses were performed using DAVID, and the results were visualized using bioinformatics and Omicshare platforms. The researchers also constructed and analyzed the "active ingredient-target-disease" network model using Cytoscape 3.8.0 software. Finally, AutoDock and Pymol software were used for molecular docking of important components and core targets to predict their binding ability. [Results] The researchers screened 27 active ingredients from Eucommia ulmoides, and found 206 common targets between atherosclerosis and active ingredients. These targets were mainly related to the Protestations in cancer, Prostate cancer, and Endocrine resistance pathways, among which AKT1 had the highest affinity for β-sitosterol. [Conclusion] This study suggests that multiple active ingredients in Eucommia ulmoides extract may exert various effects in the treatment of atherosclerosis. The findings provide a scientific basis for further development of clinical applications of Eucommia ulmoides extract.
Childhood obesity (CO) is an increasing public health issue. Mounting evidence has shown that gut microbiota (GM) is closely related to CO. However, the causal association needs to be treated with caution due to confounding factors and reverse causation.Data were obtained from the Microbiome Genome Consortium for GM as well as the Early Growth Genetics Consortium for childhood obesity and childhood body mass index (CBMI). Inverse variance weighted, maximum likelihood, weighted median, and MR.RAPS methods were applied to examine the causal association. Then replication dataset was used to validate the results and reverse Mendelian randomization analysis was performed to confirm the causal direction. Additionally, sensitivity analyses including Cochran's Q statistics, MR-Egger intercept, MR-PRESSO global test, and the leave-one-out analysis were conducted to detect the potential heterogeneity and horizontal pleiotropy.Our study found suggestive causal relationships between eight bacterial genera and the risk of childhood obesity (five for CO and four for CBMI). After validating the results in the replication dataset, we finally identified three childhood obesity-related GM including the genera Akkermansia, Intestinibacter, and Butyricimonas. Amongst these, the genus Akkermansia was both negatively associated with the risk of CO (OR = 0.574; 95% CI: 0.417, 0.789) and CBMI (β = -0.172; 95% CI: -0.306, -0.039).In this study, we employed the MR approach to investigate the causal relationship between GM and CO, and discovered that the genus Akkermansia has a protective effect on both childhood obesity and BMI. Our findings may provide a potential strategy for preventing and intervening in CO, while also offering novel insights into the pathogenesis of CO from the perspective of GM.
Epithelial ovarian cancer (EOC) remains a major gynecological problem, with a poor 5-year-survival rate due to distant metastases. The identification of microRNAs (miRNAs) may provide a novel avenue for diagnostic and treatment regimens for EOC. Several miRNAs have been reported to be involved in the progression of EOC, among which miRNA (miR)-137 has been observed to be downregulated in the ovarian tissues of patients with EOC. However, the functions of miR-137 in EOC cell apoptosis, migration and invasion remain to be elucidated. In the present study, the expression of miR-137 was measured in clinical ovarian cancer specimens and cell lines using reverse transcription-quantitative polymerase chain reaction. The role of miR-137 in the growth and survival of the SKOV3 human ovarian cancer cell line was determined using several in vitro approaches and in nude mouse models. The results demonstrated that the expression of miR-137 was downregulated in the ovarian cancer specimens and cell lines. It was also observed that enforced expression of miR-137 in the EOC cell lines decreased cell proliferation, clonogenicity, migration and invasion, and induced G1 arrest and cell apoptosis in vitro. Notably, the enforced expression of miR-137 suppressed tumor growth in the nude mice models. These findings suggested that miR-137 may act as a tumor suppressor and be used as a potential therapeutic agent for the treatment of EOC.
Following the publication of this paper, it was drawn to the Editors’ attention by a concerned reader that certain of the Transwell cell migration data shown in Fig. 4 were strikingly similar to data appearing in different form in other articles by different authors. Owing to the fact that the contentious data in the above article had already been published elsewhere, or were already under consideration for publication, prior to its submission to Molecular Medicine Reports, the Editor has decided that this paper should be retracted from the Journal. The authors were asked for an explanation to account for these concerns, but the Editorial Office did not receive any reply. The Editor apologizes to the readership for any inconvenience caused. [the original article was published in Molecular Medicine Reports 12: 5443‑5448, 2015; DOI: 10.3892/mmr.2015.4032]
Following the publication of this paper, it was drawn to the Editors’ attention by a concerned reader that the Transwell migration and invasion assay data shown in Fig. 3A and B were strikingly similar to data appearing in different form in other articles written by different authors at different research institutes that had either already been published elsewhere prior to the submission of this paper to Molecular Medicine Reports, or were under consideration for publication at around the same time (a few of which have already been retracted). In view of the fact that certain of these data had already apparently been published previously, the Editor of Molecular Medicine Reports has decided that this paper should be retracted from the Journal. The authors were asked for an explanation to account for these concerns, but the Editorial Office did not receive a reply. The Editor apologizes to the readership for any inconvenience caused. [Molecular Medicine Reports 12: 3121-3126, 2015; DOI: 10.3892/mmr.2015.3749]
Objective To evaluate the prevention and implementation on the control of iodine deficiency disorders(IDD),and to provide a scientific basis for the IDD objective assessment in Qianjiang district.Methods Five towns,covering the East,South,West,North and Intermediate region,were randomly selected in the whole region.Salts from 200 residents were randomly sampled and tested for iodine content.40 male and 40 female children,aged 8-10 years,were randomly selected from 5 primary schools and examined for thyroid palpation.Urine samples from 100 children,aged 8-10 years,were analyzed for the urinary iodine.150 students were investigated for their awareness of the health education situation.The study also included the iodized salt selling network,the number of sale outlets and iodized salt distribution,iodized salt supply and sales in these five towns.Results 95.5% of salts were qualified.The median urinary iodine content from the children was 245.9 μg/L,in which 66% samples had a range of 50~200 μg/L,30% had a concentration of 300 μg/L,and 3% of samples had a concentration of 50 μg/L.4.25% of children had hyperthyroidism.85.33% of Grade 5 students had the awareness of iodine deficiency disease prevention,and the average score was 4.46 out of 5.Conclusion The technical indicators of IDD have reached the standard in Qianjiang district.
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