Rohit Jain

The methanolic crude extract prepared from the fruits of Citrullus colocynthis (MCCF), its fractions and two isolated compounds namely Ursolic Acid, (compound1) and Cucurbitacin E 2-0-β-D-glucopyranoside (compound 2) were evaluated for their antibacterial activity. Broth microdilution and agar disc diffusion methods were used for the investigations. The antimicrobial assays showed that the crude extract, fractions FC I, FC II, compound 1 and compound 2 were capable of preventing the growth of the bacterial strains (standard strains and their clinical isolates). The lowest minimal inhibitory concentration (MIC) of 62.5µg/ml was recorded for the crude extract and the corresponding values for both the fractions and compounds were 25µg/ml each. The overall results provided promising baseline information for the potential use of the methanolic crude extract of C. colocynthis, its fractions and the tested compounds for the treatment of bacterial and fungal infections.

Citrullus colocynthis

Broth microdilution

Ursolic acid

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Citations (7)

Association of wnt9b rs1530364 and wnt5a rs566926 gene polymorphisms with nonsyndromic cleft lip and palate in South Indian population using deoxyribonucleic acid sequencing

Contemporary Clinical Dentistry (2020)

Rohit Jain RM Dharma MR Dinesh BC Amarnath Manjunath Hegde

Nonsyndromic cleft lip with or without cleft palate (CL/CP) is a common congenital facial malformation without any other structural or developmental abnormalities.To test the association of Wnt9B rs1530364 and Wnt5A rs566926 gene variants with the nonsyndromic CL/CP patients in South Indian population.Deoxyribonucleic acid (DNA) samples of 25 subjects with nonsyndromic cleft lip and palate (NSCLP) and 25 unrelated controls collected from the department were used for the study. Group A: DNA samples of 25 subjects NSCLP (P1-P25). Group B: DNA samples of 25 unrelated controls (C1-C25). The extracted DNA samples were subjected to polymerase chain reaction, and later, these amplified products were subjected to DNA sequencing. Results were documented in the form of electropherograms.The results indicated that there is a strong association between the presence of Wnt9B rs1530364 gene with the incidence of NSCLP. This study also suggests that the likelihood of NSCLP is higher in subjects having CC (P = 0.02) genotype for Wnt9B gene variant rs1530364.We can conclude that Wnt9B gene variant rs1530364 can be considered as genetic marker for NSCLP for our population.

MTRR

10.4103/ccd.ccd_90_19

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Citations (2)

Unveiling the interaction of divergent abiotic stresses and their consequences in terms of bacosides in Bacopa monnieri (L.) Wettst.

Journal of Applied Research on Medicinal and Aromatic Plants (2022)

Abhishek Dadhich Mamta Dhiman Lakshika Sharma Rajesh Kumar Rohit Jain

Bacopa monnieri

10.1016/j.jarmap.2022.100423

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Citations (5)

Correction to: Synergistic effect of cytokinins and auxins enables mass clonal multiplication of drumstick tree (Moringa oleifera Lam.): a wonder