Hadjira Belkahla

University of Blida

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Dhaouya Nebih Hadj-sadok

University of Blida

Djamila Larbi

Noureddine Bouras

École Normale Supérieure de Kouba

Kinza Benazzouz

Mouloud Mammeri University of Tizi-Ouzou

Mahfoud Bakli

University of Ghardaia

Marie-Pierre Dubrana

Biologie du Fruit et Pathologie

Sarah Bouzidi

Centre National de la Recherche Scientifique

A.M. D’Onghia

International Centre for Advanced Mediterranean Agronomic Studies

Khalissa Felkai

University of Blida

Ahlem El Ferran

École Nationale Supérieure Agronomique

Cooperative Institutions

University of Blida

École Nationale Supérieure Agronomique

Istituto Agronomico Mediterraneo di Bari

Laboratoire de Génie Chimique

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

Centre National de la Recherche Scientifique

Institut de Recherche pour le Développement

International Centre for Advanced Mediterranean Agronomic Studies

Institut National Polytechnique de Toulouse

Université Toulouse III - Paul Sabatier

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Effect of Citrus Stubborn Disease (Spiroplasma Citri) on Chemical Composition of Orange (Citrus Sinensis (L) Osbeck) Essential Oil Fruits

International Journal of Fruit Science (2020)

Kinza Benazzouz Hadjira Belkahla Sonia Hamiche Mohamed El Hattab

The effect of Citrus stubborn disease (CSD), caused by Spiroplasma citri, on chemical composition and antimicrobial activities of the essential oils of (Citrus sinensis (L) Osbeck) was studied. The results showed that the essential oil extracted from both healthy and Citrus fruit infected by the stubborn disease did not have the same physicochemical characteristics, but have identical chemical composition, with octanal, β-phellandrene, d-limonene, 1 r-α pinene, and d-limonene as major compounds. The infected fruits contained new toxic compounds (2-cyclohexen-1-ol, 2-methyl-5-(1-methylethenyl)-cis, decanal, trans-p-mentha-2, 8-dienol, 2-cyclohexen-1-ol, and, 1-methyl-4-[1-methy-4-(1-methyletheyl)]-cis), at low concentrations (<1%). Both essential oils showed very interesting antibacterial activity against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, and Pseudomonas aeruginosa ATCC 27853. However, they did not exert any antifungal activity against Botrytis cinerea. The minimum inhibitory concentration (MIC) for the bacterial strains, which were sensitive to the essential oil extracted from both infected and uninfected C. sinensis, was of 4 µL and 32 µL/mL, respectively.

Citrus × sinensis

Decanal

10.1080/15538362.2020.1791303

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Serodetection of Citrus Tristeza Closterovirus (Ctv) in Algeria.

AMERICAN-EURASIAN JOURNAL OF SUSTAINABLE AGRICULTURE (2013)

Hadjira Belkahla Djamila Larbi L. Bouafia R. Moudoud F. Guettouche

Closterovirus

Citrus tristeza virus

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Effect of aqueous extracts of two species of Algerian armoise (Artemisia herba alba and A. judaica) in vitro on the larvae (l2) of Meloidogyne.

Dhaouya Nebih Hadj-sadok Hadjira Belkahla N. Kheir

Artemisia

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MONITORING CITRUS TRISTEZA VIRUS (CTV) IN ALGERIA AND CHARACTERIZATION OF LOCAL ISOLATES

Acta Horticulturae (2015)

Djamila Larbi Hadjira Belkahla K. Djelouah A.M. D’Onghia

Citrus tristeza virus

Closterovirus

10.17660/actahortic.2015.1065.96

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Serodetection of viruses associated to barley yellow dwarf (BYD) on cereals in Algeria

Phytoprotection (2005)

Hadjira Belkahla Hervé Lapierre

Surveys on viruses associated with Barley Yellow Dwarf (BYD) and their vectors were carried out in Algerian cereal areas (Guelma, Constantine, Algiers, Sidi-belabes, Adrar) in 1997 and 1998. Rhopalosiphum padi was present in all zones of culture, whereas R. maidis, Sitobion avenae, S. fragariae and Schizaphis graminum had only local distributions. In most areas BYD-like symptoms, i.e. dwarfing and yellowing of barley ( Hordeum vulgare ), dwarfing and reddening of oat (Avena sativa) and wheat ( Triticum aestivum ), were observed. Serological tests were done on these crops using DAS-ELISA (RMV and SGV) or TAS-ELISA using monoclonal antibodies specifie to CYDV-RPV or using different variant specifie BYDV-PAV (CpA and CpB) and BYDV-MAV monoclonal antibodies. BYDV-PAV was prevalent and few plant samples carrying RMV, SGV, BYDV-MAV or CYDV-RPV were detected. The relative frequencies of BYDV-PAV CpA and CpB serotypes were variable depending on the area and the crop season. The range of symptoms induced on barley by both Algerian BYDV-PAV CpB and BYDV-PAV CpA serotypes was mild to severe. Twenty-one BYDV-MAV isolates were compared using monoclonal antibodies, which distinguish two serotypes of this virus. Only one serotype was detected. This same serotype is also the most prevalent in Europe.

Barley yellow dwarf

Dwarfing

Rhopalosiphum maidis

Luteovirus

Rhopalosiphum padi

10.7202/706190ar

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Variations temporelles et structure trophique des communautés de nématodes associées a la culture de chou (Brassica oleracea) en Algerie

Nematologia mediterranea (2011)

Dhaouya Nebih Hadj-sadok Hadjira Belkahla Z. El Aimouche

Les variations temporelles des communautes des nematodes du sol ont ete etudiees sur les cultures de chou (Brassica oleracea) dans la Mitidja en Algerie. Les echantillons de sol ont ete collectes mensuellement du mois de novembre 2008 au mois de mai 2009, sur deux varietes de chou vert (Enkuisen, Compne haguen) et une variete de chou rouge. Douze taxons ont ete identifies, parmi les genres dominants sur les trois varietes de chou, nous avons note Aphelenchus et Ditylenchus (fungivores) et Tylenchorhynchus et Pratylenchus dans le groupe des phytophages. Des differences significatives ont ete observees entre le temps (P < 0.01), pour l’abondance absolue des nematodes et des groupes trophiques a l’exception du groupe phytophage.

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Application of Real Time PCR for the Prevalence Determination of Citrus Exocortis Viroid (CEVd) in Algeria

Advanced Research in Life Sciences (2023)

Ahlem El Ferran Hadjira Belkahla Noureddine Bouras Ali Atoui Mahfoud Bakli

Abstract Citrus exocortis is a grafting disease caused by Citrus Exocortis Viroid (CEVd). The knowledge of the viroid’s incidence and distribution are necessary to further apply control measures. The objective of this work was to apply the real time PCR assay for the detection of CEVd in samples collected from symptomatic CEVd-infected plants in Mitidja (North Algeria). The assay showed an excellent diagnostic specificity where 38 out of 50 samples showed a positive reaction for CEVd, which revealed the presence of CEVd in citrus orchards with a prevalence of 76%. Consequently, this work offers a quick alternative to conventional methods for the early diagnosis and the prevalence assessment of CEVd.

10.2478/arls-2023-0008

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Citations (2)

Identification and Detection of Citrus Psorosis Virus (CPsV) In Citrus Varieties And Rootstocks Cultivated In Mitidja Region, Algeria

Advanced Research in Life Sciences (2024)

Khalissa Felkai Amine Yekkour Hadjira Belkahla Noureddine Bouras Mahfoud Bakli

Abstract Psorosis is one of major graft-transmissible diseases of Citrus ; the virus was reported to occur in different Citrus cultivated areas and constitutes a serious threat to Citrus establishment and development. This study focuses on the identification of Citrus Psorosis Virus (CPsV) by tow diagnostic methods; biological indexing in indicator seedling and serological test (DAS-ELISA). Thereby, different varieties of sweet orange ( Citrus sinensis (L.) Osbeck ), mandarin ( Citrus × Deliciosa ), lemon ( Citrus × limon ), Grapefruits ( Citrus × Paradisi ), clementine ( Citrus × Clementine ) and tow rootstocks sour orange ( Citrus × aurantium L.) and trifoliate orange ( Poncirus trifoliata) that exhibit typical symptoms of Psorosis disease have been detected and collected from five different orchards located in the regions of Mitidja (Algeria). All collected samples were subjected to biological testing and double antibody sandwich ELISA (DASELISA) as diagnostic tests. The serological method, specifically DAS-ELISA, yielded positive results on varieties from the prospect station, indicating the presence of psorosis in Algeria. The typical symptoms of psorosis were observed through the indexed seedlings and grafts.

Identification

10.2478/arls-2024-0006

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EFFET DES EXTRAITS AQUEUX DE DEUX ESPECES D’ARMOISE ALGERIENNE (ARTEMISIA HERBA ALBA ET ARTEMESIA JUDAICA) IN VITRO SUR LES LARVES (L2) DE MELOIDOGYNE

AGROBIOLOGIA (2014)

Dhaouya Nebih Hadj-sadok Nawel Kheir Hadjira Belkahla

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Occurrence of Spiroplasma citri in Algerian Citrus Orchards

Research Square (Research Square) (2023)

Kinza Benazzouz Hadjira Belkahla Marie-Pierre Dubrana Arezki Lehad

Abstract Algeria is a country known for a varietal variety of Citrus fruits, which are susceptible to serious pathologies such as stubborn disease ( Spiroplasma citri ), causing significant economic losses. The current study was conducted in order to investigate the possible existence of different Spiroplasma citri strains in Algeria. Samples of fresh leaves and orange-tree fruits ( Citrus sinensis L.) with stubborn symptoms were detected in Boumerdes and Blida province. Identification of the causative agent ( Spiroplasma citri ) was ensured by ELISA and PCR techniques. DNA extracted from symptomatic samples were used as a template for amplification of products of 675 bp using primer pairs Spiralin (Sc1 / Sc1-) and (SpiF / SpiR) by PCR. Furthermore, the spiralin gene was cloned and sequenced. About 18,23% of the samples, showing clear-cut stubborn symptoms, were ELISA - positive. Molecular characterization by sequence analysis has identified two Algerian isolates with 99% homology between them. Spiroplasma citri Isolate 12 (OQ078580) and Spiroplasma citri isolate 14 (OQ068387). Phylogenetic tree showed that the Algerian Spiroplasma citri isolates are more closely related to the Egypt, Israel, than those isolated from Iran and Corse.

Spiroplasma

Phylogenetic relationship

Citrus × sinensis

10.21203/rs.3.rs-2503249/v1

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Citations (1)