In order to accelerate the application and development of laser welding in the car body-in-white in auto industry and improve the strength of BIW and different testing demands of car,it is the first time to study the laser welding joint designing types and set up the national quality assessment standards of the laser welding joints systematically and deeply.Through quite a few laser welding tests,mechanical test,microstructure test of ultra strength steel,galvanized sheet and 3-layer sheet,the database of the joint designing types and assessment standards are finished in order to make the quality of welded beam stable and meet with the requirement of product designing.For the welding of the 3-layer sheet and galvanized sheet,the application of the ultra strength sheet and national crash regulation,it is very important so that the designing demand can be meeted for the front crash,rear crash and side crash.
The rock excavation in the deep and long tunnels of Jinping second stage hydropower station(depth 2 525 m,length 19 km,width 13 m) showed an obvious deterioration to its rheological properties.The CSS-283 bi-direction universal servo testing machine is used to conduct uniaxial compression creep test and shear creep test for the surrounding rock(soft slate).The respective rheological model of rock for shear and uniaxial compression creep processes is established by introducing the rheological parameters which are functions of hardening and time.This nonlinear creep model presents the expression of strain equation,which is a together of four individual strain parts,including the instantaneous elastic deformation,viscoelastic deformation,instantaneous plastic deformation and viscoplastic deformation.What's more,those parameters in the strain equation are non-stationary,they are correlated with time and stress.Besides,the harding characteristic of rock samples is also considered during the behaviors of their creep process.Based on that the corresponding three-dimension model is therefore developed,the theoretical calculated results are well consistent with the test results.Finally,numerical simulation is performed to study the excavation of deep tunnels of Jinping hydropower station and the tunnel deformation after the 190 days' excavation is also obtained.Accordingly,these investigation results can provide a theoretical guidance for the engineering construction.
Combining with the retaining wall design in the water system works at Beitang District of Tianjin,the study explores the treatment scheme for the deep soft foundation under the retaining walls,compares the calculation of the loading capacity of the mixing piles and the composite foundation,and analyzes the influence of the mixing pile layout on the anti-sliding and stability of the retaining walls,so as to accumulate relative experience and direct similar projects.
J. Neurochem. (2012) 120 , 842–849. Abstract Endocannabinoids are neuromodulatory lipids that mediate the central and peripheral neural functions. Endocannabinoids have demonstrated their anti‐proliferative, anti‐angiogenic and pro‐apoptotic properties in a series of studies. In the present study, we investigated the levels of two major endocannabinoids, anandamide and 2‐arachidonylglycerol (2‐AG), and their receptors, CB1 and CB2, in human low grade glioma (WHO grade I‐II) tissues, high grade glioma (WHO grade III‐IV) tissues, and non‐tumor brain tissue controls. We also measured the expressions and activities of the enzymes responsible for anandamide and 2‐AG biosynthesis and degradation, that is, N ‐acylphosphatidylethanolamine‐hydrolysing phospholipase D (NAPE‐PLD), fatty acid amide hydrolase (FAAH), monoacylglycerol lipase (MGL), and diacylglycerol lipase‐alpha (DGL), in the same samples. Liquid chromatography–mass spectometry analysis showed that the levels of anandamide decreased, whereas the levels of 2‐AG increased in glioma tissues, comparing to the non‐tumor controls. The expression levels and activities of NAPE‐PLD, FAAH and MGL also decreased in glioma tissues. Furthermore, quantitative‐PCR analysis and western‐blot analysis revealed that the expression levels of cananbinoid receptors, CB1 and CB2, were elevated in human glioma tissues. The changes of anandamide and 2‐AG contents in different stages of gliomas may qualify them as the potential endogenous biomarkers for glial tumor malignancy.
Abstract [Background] Resveratrol is a polyphenol present abundantly in lots of traditional Chinese medicines, which has been shown to have beneficial effects on neurological diseases. However, the molecular mechanisms of resveratrol on traumatic brain injury have not been systematically studied yet. In this study, we elucidated the pharmacological mechanisms of resveratrol in treating traumatic brain injury by using a network pharmacology method. [Methods] The pharmacokinetics properties of resveratrol were obtained from the traditional Chinese medicine systems pharmacology database and analysis platform (TCMSP). The putative targets of resveratrol were obtained from TCMSP, BATMAN-TCM, SuperPred, PharmMapper, SwissTargetPrediction, DrugBank, and a literature search. The targets related to traumatic brain injury were obtained from TTD, DrugBank, CTD, GeneCards, OMIM, MalaCards, and a literature search. The STRING database and the Cytoscape 3.8.0 software were used to build the protein-protein interaction (PPI) network. The Metascape database was used to obtain the gene ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment information. Finally, The AutoDockTools 1.5.6 and PyMOL 2.4.0 software were employed for molecular docking analyses, and Discovery Studio 2020 was used for interaction analyses. [Results] A total of 165 overlapping targets involved in resveratrol intervention in traumatic brain injury were determined. The GO function analysis indicated that the targets are including the positive regulation of transferase activity, the positive regulation of cell migration, reactive oxygen species metabolism, the wound response, and so on. The KEGG pathway analysis identified the following enriched pathways: the AGE-RAGE signalling pathway, the FoxO signalling pathway, insulin resistance, complement and coagulation cascades, the HIF-1 signalling pathway, and so on. According to the PPI network analysis, INS, IGF1, TNF, TP53, ALB, IL6, SRC, STAT3, VEGFA, and MMP9 were identified as hub target genes, in which IL6, MMP9, INS, and SRC showed a good binding affinity with resveratrol in molecular docking. [Conclusions] Resveratrol may target multiple genes and multiple pathways to reduce brain damage after traumatic brain injury.
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