J Wang

South China Agricultural University

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Ming Wen

Chongqing Three Gorges University

Ren Z

Guangzhou University of Chinese Medicine

Yan Li

University of Southern Queensland

M Wang

Liangjun Yin

Guangxi University of Science and Technology

Yin J

Jiangnan University

Dawei Han

University of Bristol

Ren Z

Ludong University

Jiang Gu

Shantou University

Yuchao Cui

Xiamen University

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Sun Yat-sen University

University of Electronic Science and Technology of China

Chongqing Medical University

Capital Medical University

Dalian Medical University

Second Affiliated Hospital of Chongqing Medical University

Chinese Academy of Sciences

Tsinghua University

South China Agricultural University

Chongqing Three Gorges University

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[High level expression of human recombinant albumin gene in the yeast Pichia pastoris].

PubMed (2001)

Yuchao Cui Ming Wen Wei Yin Ren Z J Wang

The yeast Pichia pastoris was transformed by the multi-copy Pichia expression vector that can express secreted human albumin. The high level expression of cell line was selected after screening. The expression of human recombinant albumin in Pichia pastoris induced by different methods were compared. The ratio of secreted human albumin is 80% in total secreted proteins and the expression level reaches as high as is 10 g/L.

Pichia

Human serum albumin

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FGF5 promotes osteosarcoma cells proliferation via activating MAPK signaling pathway

DOAJ (DOAJ: Directory of Open Access Journals) (2019)

Dawei Han M Wang Yù Zhang Liangjun Yin Liu C

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Study on the Interaction Mechanism between Galactomannan and Human Serum Albumin by Spectroscopy and Molecular Docking