J.M.S.G. Martinho

Universidade Federal Fluminense

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L.F. Pacheco-Moreira

Hospital Geral de Bonsucesso

Damião Carlos Moraes dos Santos

Fundação Oswaldo Cruz

Cristina Carvalho Viana de Araújo

Hospital Geral de Bonsucesso

Ana Maria Reis Ferreira

Universidade Federal Fluminense

Gilson Teles Boaventura

Universidade Federal Fluminense

Marcelo Pelajo‐Machado

Fundação Oswaldo Cruz

Mario Pinto

Shamnur Shivashankarappa Institute of Medical Sciences & Research Centre

Giuseppe Santalucia

Hospital Geral de Bonsucesso

Elzinandes Leal de Azeredo

Fundação Oswaldo Cruz

Patricia C. C. Neves

Instituto de Tecnologia e Pesquisa

Cooperative Institutions

Fundação Oswaldo Cruz

Universidade Federal Fluminense

Hospital Geral de Bonsucesso

Faculdades Oswaldo Cruz

Ministério da Saúde

Universidade Federal do Estado do Rio de Janeiro

Universidade do Estado do Rio de Janeiro

International Olive Council

Universidade Federal do Rio de Janeiro

Universidade Federal de Mato Grosso do Sul

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Activated lymphocytes and high liver expression of IFN‐γ are associated with fulminant hepatic failure in patients

Liver International (2011)

Damião Carlos Moraes dos Santos Patricia C. C. Neves Elzinandes Leal de Azeredo Marcelo Pelajo‐Machado J.M.S.G. Martinho

To study immunological mechanisms of fulminant hepatic failure (FHF) derived from extensive liver lesions, 14 patients with FHF induced by different aetiologies were investigated by observance of both lymphocyte phenotyping and cytokine levels.Five patients bearing benign acute hepatitis B (AHB) and seven healthy liver donors (HC) were used as controls. Samples of liver and blood from both FHF patients and HC were obtained during transplantation procedures. Plasma levels of IL-1β, IL-4, IL-6, IL-8, IL-10, IFN-γ, TNF-α, MCP-1, RANTES and MIP-1α were quantified using a multiplex immunoassay. Cell characterization was carried out by flow cytometry. IFN-γ staining was performed on liver sections using immunofluorescence methods.An increase of peripheral frequency of natural killer (NK) cells expressing early activation markers (CD69, HLA-DR and CD38) and adhesion molecule CD44 was observed in FHF patients. Elevated frequency of T lymphocytes CD4(+) and CD8(+) expressing CD38 and adhesion molecules CD29 and CD44 was also observed in FHF. Additionally, an increase of natural killer T cells (NKT) was detected in FHF patients. High plasma cytokine levels were not statistically different between FHF and AHB patients. In comparison to HC, a strong liver expression of IFN-γ was detected in FHF patients. The increased frequency of CD4(+) CD44(+) and IL-8 cytokine was found in patients with poor prognosis.These findings indicate the involvement of NK and NKT cells as well as T lymphocytes CD4(+) and CD8(+) in the inflammatory process inducing FHF, confirmed by the high hepatic expression of IFN-γ.

Fulminant hepatic failure

CD16

10.1111/j.1478-3231.2011.02654.x

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Citations (18)

P.431 Immunological changes in liver parenchyma and peripheral blood cells from patients with fulminant hepatic failure

Journal of Clinical Virology (2006)

Damião Carlos Moraes dos Santos J.M.S.G. Martinho L.F. Pacheco-Moreira Cristina Carvalho Viana de Araújo M Pelajo-Machado

Fulminant hepatic failure

Fulminant

Parenchyma

Liver parenchyma