Direct seeding of hot pepper can save labor comparing with the method that use transplants. The objective of this study was to evaluate effects of seeding space, depth, date, and low tunnel cultivation on yields of once-over harvested hot pepper (‘Jalapeno’ × ‘Indo’ and ‘9837059’). Hot peppers were direct-seeded in single or double rows at various seeding depths on several planting dates. Seeds also were sown inside and outside plastic tunnels. There were no significant effects of seeding space, depth and date on plant stand or yield, although early seeding and shallow seeding depths tended to increase both these variables. The best treatment combinations for plant stand and yield was using a double row 60 ㎝ apart (on a 120 ㎝ wide bed) with 20 ㎝ in-row spacing and 0.3 ㎝ planting depth. Low tunnel cultivation also increased stand rate and yield of hot pepper.