T Muzashvili

Tbilisi State Medical University

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George Burkadze

Tbilisi State Medical University

Mariam Gachechiladze

Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. - Hans-Knöll-Institut (HKI)

R Beriashvili

Tbilisi State Medical University

George Tevdorashvili

Tbilisi State Medical University

N Kikalishvili

Tbilisi State Medical University

G Pkhakadze

Tbilisi State Medical University

Zaza Bokhua

Tbilisi State Medical University

T Asatiani

Tbilisi State Medical University

N Kintraia

Tbilisi State Medical University

ТІ

Тетяна Іванівна Кікнадзе

Zaporizhia State Medical University

Cooperative Institutions

Tbilisi State Medical University

University of Salerno

Université du Québec à Chicoutimi

Institute of Soil Science and Plant Cultivation

Boréa (Sweden)

University of Veterinary Medicine Vienna

Indena (Italy)

South Valley University

Petre Shotadze Tbilisi Medical Academy

David Tvildiani Medical University

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Helleborus caucasicus A.Br.-კავკასიური ხარისძირას მიწისქვეშა ნაწილების სტეროიდული ნივთიერებების შესწავლა

T Muzashvili

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APPLICATION OF ULTRASOUND TECHNOLOGY IN THE PROCESSING OF HISTOLOGICAL MATERIAL.

PubMed (2024)

G Burkadze N Kikalishvili T Muzashvili

The pathological examination is one of the longest in the list of medical tests. Most of this time is spent on preparation for the slide. In addition, part of the chemical reagents needed for processing is not safe for the environment and the personal working in the laboratory. The goal of our project was to develop a new, cost-effective and eco-friendly tissue processing technology by integrating a new hardware component - an ultrasound device - into the processing protocol. 28 experimental protocols were developed to create a new tissue material processing protocol. A selected broad panel of tissues was used for each experiment. 168 autopsy tissue samples with a standard set of organs were used in the examination. Processing was carried out manually under conditions of ultrasonic treatment (UP200HT). All slides were stained with hematoxylin-eosin technology. The quality of the slides was evaluated through 5 points evaluation system by 2 pathologists. Application of ultrasound technology in the processing of histological material will: reduce processing time and number of reagents required for processing; increase the cost efficiency of processing; remove harmful component xylene from the processing protocol; better preserve tissue antigenic structure at the expense of reduced tissue retention time in reagents. So, the interdisciplinary use of engineering and medicine will be of great benefit to both the medical staff and the environment, as well as to patients (they will receive better research quality and reduced waiting time for examination results).

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PHENOTYPIC CHARACTERISTICS OF RELAPSED LEIOMYOMA AND SMOOTH MUSCLE TUMORS OF UNCERTAIN MALIGNANCY POTENTIAL IN REPRODUCTIVE WOMEN.

Georgian Medical News (2021)

Тетяна Іванівна Кікнадзе George Tevdorashvili T Muzashvili Mariam Gachechiladze George Burkadze

Uterine leiomyoma represents the most common pelvic tumor in females, including numerous histological subtypes, from which smooth muscle tumors of uncertain malignancy potential (STUMP) represents the diagnostic challenge. On the other hand, the study of the relapse risk markers after laparoscopic myomectomy is of high interest. We investigated the molecular phenotype of different types of leiomyoma after hysterectomy or laparoscopic surgery in reproductive and menopausal women. Standard immunohistochemistry was used to detect proliferation markers Ki67 and cyclin D1, apoptotic markers Bcl2 and Cas3, and ER and PR. The results of our study indicated that ER expression is significantly higher in relapsed leiomyoma, compared to control group. In addition, relapsed leiomyomas are characterised with high proliferation and apoptotic index. With regard to STUMP despite histological homogeneity, this entity is characterised with the presence of three distinct molecular subtypes, based on proliferation and apoptotic marker expression, which should be used as diagnostic aid in these tumors.

Uterine Leiomyoma

Smooth Muscle Tumor

Ki-67

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Ultraperformance Liquid Chromatography Tandem Mass Spectrometry Determination of Cyanogenic Glucosides in Trifolium Species

Journal of Agricultural and Food Chemistry (2014)

T Muzashvili Barbara Moniuszko‐Szajwaj Łukasz Pecio Wiesław Oleszek Anna Stochmal

Cyanogenic glucosides were analyzed by ultra-high-performance liquid chromatography combined with mass spectrometry in 88 Trifolium species grown at the same site. On the basis of the occurrence of cyanogenic glucosides and the linamarin/lotaustralin ratio species could be grouped into five clusters. Cluster C1 included 37 species, which did not contain cyanogens. Cluster C2 (22 species) included plants containing only lotaustralin. In clusters C3 (14 species), C4 (13 species), and C5 (2 species) both linamarin and lotaustralin were present but at different ratios. In C3 and C4 the linamarin/lotaustralin ratio was below 1, whereas in cluster C5 the ratio was much higher. Generally, the total content of cyanogens was below 500 μg/g dry matter. Only in Trifolium repens var. biasoletti and Trifolium montanum extremely high cyanogen concentrations were observed. There was no general rule of occurrence of cyanogens. Samples of the same species from different countries accumulated cyanogens or could be free of these compounds.

Trifolium repens

Cyanogen

Dry weight

10.1021/jf4056659

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EVALUATION OF THE RISK OF CERVICAL INTRAEPITHELIAL NEOPLASIA PROGRESSION BASED ON CELL PROLIFERATION INDEX, EPITHELIAL-MESENCHYMAL TRANSITION AND CO-INFECTIONS.

PubMed (2020)

G Pkhakadze Zaza Bokhua T Asatiani T Muzashvili George Burkadze

Human papilloma virus (HPV) infection, especially with high risk types, represent the major etiological factor for the development of cervical precancerous and cancerous lesions. However, other factors including cell proliferation index, epithelial-mesenchymal transition and the presence of co-infections might also influence the progression of cervical intraepithelial neoplasia (CIN). The aim of our study was to analyse, the expression of cell proliferation markers and epithelial-mesenchymal transition markers during the progression of cervical intraepithelial neoplasia, in cases with and without co-infections. Standard immunohistochemistry was used to detect, Ki67, cyclin D1, phosphohiston-H3, p63, E-cadherin, β-catenin and vimentin. The results of our study indicated that the expression of Ki67, phosphohiston-H3 and p63 is significantly increased during the progression of CIN disease, whilst the expression of E-cadherin and β-catenin are progressively lost. The expression of mesenchymal marker vimentin is also increased in CINIII and in invasive carcinoma. Proliferation index based on Ki67 labelling is significantly higher in cases with co-infections and the expression on E-cadherin is significantly lower in cases with co-infections compared to cases without co-infections. In conclusion, the measurement of proliferation index, based on Ki67 labelling, as well as mitotic index based on phosphohiston-H3 detection can reliably indicate high and low risk groups of the progression of CIN. Similarly, higher p63 expression, loss of E-cadherin and β-catenin and higher vimentin expression can indicate the progression risk of CIN. The presence of co-infections is associated with the increased expression of proliferation marker Ki67 and the loss of E-cadherin and therefore it can be considered as an additional marker of CIN progression.

Proliferation Marker

Proliferation index

Ki-67

Proliferative index

Tumor progression

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Citations (1)

Isolation of a new disaccharide nucleoside from Helleborus caucasicus: structure elucidation and total synthesis of hellecaucaside A and its β-anomer

Carbohydrate Research (2014)

Balla Sylla Charles Gauthier Jean Legault Pierre-Yves Fleury Serge Lavoie

Disaccharide

Natural product

Deoxyadenosine

10.1016/j.carres.2014.06.027

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Citations (14)

MICROENVIRONMENT ALTERATIONS IN CONJUNCTIVAL NEOPLASTIC LEOSIONS WITH DIFFERENT PROLIFERATION-APOPTOTIC CHARACTERISTICS.

Georgian Medical News (2021)

N Nikolaishvili G Chichua T Muzashvili George Burkadze

Different studies indicate that tumor infiltrating lymphocytes (TILs) and tumor associated neutrophils (TANs) play an important role during the progression of malignant tumors. We have analysed the distribution of tumor associated neutrophils (TANs) and tumor infiltrating lymphocytes (TILs) in different conjunctival lesions, with different proliferation and apoptotic characteristics. The distribution of TILs and TANs were evaluated in standard haematoxylin and eosin (H&E) stained sections using the digital pathology software QuPathin normal conjunctiva, actinic keratosis, pterigea, conjunctival intraepithelial neoplasias (CoIN1-3) and conjunctival squamous cell carcinoma (CSCC). In addition, the expression of following markers were investigated using standard immunohistochemistry: Ki67, Bcl2, p53, CD3, CD8 and Foxp3. The study results indicated that the number of TILs and TANs are significantly increased during the progression of conjunctival intraepithelial lesions. Also, the number of TILs and TANs significantly correlate with higher proliferation index, lower apoptotic index and the p53 mutation status.

Proliferation index

Pagetoid

Actinic keratosis

Haematoxylin

Tumor-infiltrating lymphocytes

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Advances in the biotechnological glycosylation of valuable flavonoids

Biotechnology Advances (2014)

Jianbo Xiao T Muzashvili Milen I. Georgiev

Biotransformation

10.1016/j.biotechadv.2014.04.006

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Citations (294)

PROLIFERATIVE/STEM CELL INDEX AND PHENOTYPIC CHARACTERISTICS OF PROLIFERATIVE PROCESSES IN ENDOMETRIUM.

PubMed (2018)

N Kikalishvili R Beriashvili T Muzashvili George Burkadze

The aim of the study was to explore and identify the relationship between endometrial proliferative/stem cell index and phenotypic characteristics under proliferative processes of endometrium using statistical correlation analysis. The study represents a retrospective research. The coded and depersonalized material data from Acad. N. Kipshidze Central University Clinic was used in the study. 5 study groups (83 cases) were selected from routine histopathology tissue specimens of uterus. Hematoxilyn-eosin technology and immunohistochemistry with markers ki67, CD146, PTEN was performed. The proliferative/stem cell index (PR/ST index) was calculated by the ratio of Ki67-positive cell percentage value divided by CD146-positive cell percentage value. PTEN expression score was evaluated by next scheme - PTEN positive cell numbers <10% - conditional negative, PTEN positive cell numbers 10-50 % - heterogenic, PTEN positive cell numbers >50% - conditional positive. The study showed that, PR/ST index in 1st study group Endometrial Hyperplasia ranges within the interval 15-23, PTEN is diffusely positive with focal heterogeneity 11.1%. 2nd study group Endometrial Dysplasia, the PR/ST index ranges within the interval 16.5-23.3. PTEN heterogeneity is 18.3%, negativity - 6.3%. 3rd study group Endometrioid Carcinoma Grade 1, the PR/ST index ranges between 21.7 and 25.5. PTEN is heterogenic in 35.7% of cases, negative in 14.3% of cases. 4th study group Endometrioid Carcinoma Grade 2, the PR/ST index ranges within the interval 23.2-27.8. PTEN is heterogenic in 43.5% of cases, negative in 30.4% of cases. 5th study group Endometrioid Carcinoma Grade 3, the PR/ST index ranges within the interval 25.8-29.4. PTEN is heterogenic in 33.3% of cases, negative in 41.7% of cases. It was found that, in endometrial hyperplasia and dysplasia cases the PR/ST index do not correlate with phenotypic characteristics, while in the cases of endometrial carcinoma different degrees of malignancy the PR/ST Index and phenotypic characteristics intensely and directly correlates. The high attention should be given to the fact that heterogeneity peak of PTEN expression takes place in the cases of endometrial carcinoma G2, but the negativity of PTEN protein is increasing in parallel with the malignancy increasing process and reaches peak performance in cases of endometrial carcinoma G3.

Proliferation index

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MOLECULAR MARKERS OF THE PROGRESSION OF CONJUNCTIVAL NEOPLASTIC EPITHELIAL LESIONS.

PubMed (2021)

N Nikolaishvili G Chichua T Muzashvili George Burkadze

Conjunctival epithelial lesions vary from benign to borderline malignancy to malignant, therefore it is extremely important to detect the molecular markers of the malignant progression of conjunctival intraepithelial lesions. The aim of our study was to analyse the molecular markers of the progression of conjunctival intraepithelial lesions. We have analysed Ki67, PHH3, Bcl2, P53, P63 and CK7 using standard immunohistochemistry. In addition, we have calculated the squamous-glandular index based on the evaluation of H&E stained specimens and as the ratio of P63/CK7. The results of our study indicated that the presence of squamous epithelium is significantly increased during the progression of conjunctival intraepithelial lesions, and therefore the squamous-glandular index is also increased. In addition, it is possible to divide conjunctival intraepithelial lesions as low grade and high grade lesions based on the distribution of proliferation and apoptosis markers.

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