Abstract Sweet potato, a dicotyledonous and perennial plant, is the third tuber/root crop species behind potato and cassava in terms of production. Long terminal repeat (LTR) retrotransposons are highly abundant in sweet potato, contributing to genetic diversity. Retrotransposon-based insertion polymorphism (RBIP) is a high-throughput marker system to study the genetic diversity of plant species. To date, there have been no transposon marker-based genetic diversity analyses of sweet potato. Here, we reported a structure-based analysis of the sweet potato genome, a total of 21555 LTR retrotransposons, which belonged to the main LTR-retrotransposon subfamilies Ty3-gypsy and Ty1-copia were identified. After searching and selecting using Hidden Markov Models (HMMs), 1616 LTR retrotransposon sequences containing at least two models were screened. A total of 48 RBIP primers were synthesized based on the high copy numbers of conserved LTR sequences. Fifty-six amplicons with an average polymorphism of 91.07% were generated in 105 sweet potato germplasm resources based on RBIP markers. A Unweighted Pair Group Method with Arithmatic Mean (UPGMA) dendrogram, a model-based genetic structure and principal component analysis divided the sweet potato germplasms into 3 groups containing 8, 53, and 44 germplasms. All the three analyses produced significant groupwise consensus. However, almost all the germplasms contained only one primary locus. The analysis of molecular variance (AMOVA) among the groups indicated higher intergroup genetic variation (53%) than intrapopulation genetic variation. In addition, long-term self-retention may cause some germplasm resources to exhibit variable segregation. These results suggest that these sweet potato germplasms are not well evolutionarily diversified, although geographic speciation could have occurred at a limited level. This study highlights the utility of RBIP markers for determining the intraspecies variability of sweet potato and have the potential to be used as core primer pairs for variety identification, genetic diversity assessment and linkage map construction. The results could provide a good theoretical reference and guidance for germplasm research and breeding.
Abstract Traditional architecture in agricultural heritage sites (AHSs) embodies the livelihood of local communities and residents, which is an important part of the AHS. However, with the intervention of tourism, some of the traditional architecture in AHSs has gradually transformed into simple use for tourism, without reflecting the cultural connotations of agricultural heritage systems, as well as the farming wisdom of the AHS. This paper takes Digang Food Street in Huzhou, China as an example, which is in the core protection area of the Huzhou mulberry-dyke and fish-pond system, combining comparative analysis and geographical system analysis to explore the adaptive transformation in decoration, structure, spatial layout and functional form of buildings after the development of the tourism industry. The dynamic relationship among traditional architecture, sustainable development of tourism and conservation of agriculture heritage is also analysed, expecting to provide some experience and reference for the conservation of other AHSs.
The Taihu Lake drainage basin is the birthplace of the Mulberry-dyke and Fish-pond System (MFS), a traditional eco-agricultural system. In 2017, the largest and best-preserved “Zhejiang Huzhou Mulberry-dyke and Fish-pond System” located by the South Bank of Taihu Lake, China was recognized as Globally Important Agricultural Heritage Systems (GIAHS) by the Food and Agriculture Organization of the United Nations (FAO), and its value has been appreciated. As a dynamic heritage, the sustainable development of MFS is a fundamental requirement of the conservation of GIAHS. In this regard, it is necessary to figure out an approach to evaluating the status of its sustainable development. This paper analyzes and contrasts the emergy embodied in the three patterns of MFS over different periods, then constructs an index system of sustainability evaluation involving the production and consumption processes based on that. Finally, it provides the evaluation and analysis. The three patterns of MFS differ in the system structure. In the Ming and Qing Dynasties (abbreviated as Ming-Qing pattern), MFS was an integrated system compromised of mulberry cultivation, silkworm breeding, fish breeding, and sheep breeding, while other patterns exclude sheep breeding, but increase the input of fertilizer, and add the production of mulberry-leaf tea and other local specialties. The results show that the MFS in the Ming-Qing pattern has the highest integrated evaluation index of sustainable development, followed by the traditional MFS pattern and the new MFS pattern employed nowadays. This indicates that the current capability of sustainable development has decreased compared to that in the Ming and Qing Dynasties. The integrated evaluation index regarding the consumption process of the new MFS pattern is higher than the traditional one, suggesting that it needs to promote sustainability in the production process, especially via the utilization rates of renewable resources and wastes.
Zhejiang Province, located in the Yangtze River Delta region, is representative of China′s economically developed areas. It enjoys superior natural conditions and a long history of agriculture, and is a comprehensive agricultural area with integrated development of agriculture, forestry, animal husbandry and fishery. It has nurtured the farming culture represented by Hemudu culture and Liangzhu culture, which have given rise to numerous precious Agricultural Heritage Systems. At present, Zhejiang Province has three of the world's Globally Important Agricultural Heritage Systems (GIAHS) and 12 China Nationally Important Agricultural Heritage Systems (China-NIAHS), so it not only has the largest number of heritages in China, but it has also attained remarkable achievements in heritage conservation. Taking Zhejiang Province as an example in combination with the rural revitalization strategy, this paper summarizes the achievements in the protection of Important Agricultural Heritage Systems (IAHS) in Zhejiang Province during the past 15 years from the aspects of increasing farmers' income, cultural Inheritance and industrial upgrading, as well as the conservation experiences in government promotion, community initiative, enterprise participation, technology driving and social linkage. Further, in view of the problems that exist in the current heritage protection,such as imperfect management of heritage sites, low participation of community residents, lack of special protection funds, and imperfect provincial management system,the following countermeasures and suggestions are put forward: (1) Improve the management mechanism for the conservation and development of Agricultural Heritage Systems; (2) Develop regional public branding of agricultural products in Agricultural Heritage System sites; (3) Increase the Agricultural Heritage System science education as well as cultural and creative product development; (4) Carry out the evaluation and recognition of IAHS at the provincial level; and (5) Provide substantial support and input to the conservation and utilization of IAHS. This study can provide some guidance for the conservation of IAHS in Zhejiang Province and it provides important reference for IAHS in the economically developed areas in China.
The Qingyuan mushroom culture system (QMCS) in Zhejiang Province has been recognized as the source of the world’s first artificial cultivation of mushrooms with a history of more than 800 years. The system embodies the farming wisdom of the local people who have adapted themselves to and utilized nature and has integrated the traditional bark hacking method with multiple modern patterns for mushroom cultivation based on artificial microbial strains. We have investigated the input–output operation of farmers and assessed and analyzed emergy indicators in a bid to reflect and compare their economic and ecological benefits, as well as their sustainable development by analyzing the three typical mushroom cultivation patterns. The cost-benefit analysis of the three patterns shows that the sawdust medium-cultivated method (SMCM) is characterized by dominance in both net income without labor cost and labor productivity; while the evaluation based on emergy indicators has proven that each has its own advantages and disadvantages in terms of ecological and economic benefits and sustainable development. Among them, the bark hacking method (BHM) features the highest utilization rates of local and renewable resources, the smallest damage to the environment, the lowest production efficiency, and the highest exchange efficiency, but the sawdust medium-cultivated method is just the opposite, and the log-cultivated method (LCM) is the most favorable one for sustainable development. As its agricultural heritage, the QMCS’ core of dynamic protection and adaptive management lies in enhancing the sustainable development of its agricultural production methods. It is recommended that the three patterns be improved by targeting their respective shortcomings and at the same time, integrate their advantages to explore a new sustainable development pattern for mushroom cultivation.
Abstract Sweet potato, a dicotyledonous and perennial plant, is the third most crucial tuber/root crop species behind potato and cassava in terms of production. Long terminal repeat (LTR) retrotransposons are highly abundant in sweet potato, contributing to genetic diversity. These LTR retrotransposons play a significant role in sweet potato genotypes. Retrotransposon-based insertion polymorphism (RBIP) is a high-throughput marker system to study the genetic diversity of plant species. To date, there have been no transposon marker-based genetic diversity analyses of sweet potato. We report a structure-based analysis of the sweet potato genome for the main LTR-retrotransposon subfamilies, Ty3-gypsy and Ty1-copia , which revealed a total of 21555 LTR retrotransposons. By searching using hidden Markov models (HMMs), 1616 LTR retrotransposon sequences containing at least two models were found. A total of 48 RBIP primers were synthesized based on the high copy numbers of conserved LTR sequences. RBIP markers of the genetic diversity and population structure of 105 sweet potato germplasm resources revealed 56 amplicons with an average polymorphism of 91.07%. Sweet potato accessions were collected from 6 provinces of China, Japan and America. A UPGMA dendrogram, a model-based genetic structure and principal component analysis (PCA) divided the sweet potato germplasms into 3 groups containing 8, 53, and 44 germplasms. All three analyses produced significant groupwise consensus. However, almost all the germplasms contained only one primary locus. The analysis of molecular variance (AMOVA) among the groups indicated higher intergroup genetic variation (53%) than intrapopulation genetic variation. In addition, long-term self-retention may cause some germplasm resources to exhibit variable segregation. These results suggest that these sweet potato germplasms are not well evolutionarily diversified, although geographic speciation could have occurred at a limited level. This study highlights the utility of RBIP markers for determining the intraspecies variability of sweet potato.
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