Tokarevich Nk

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

[The morphological changes in the body of guinea pigs in response to the administration of different Coxiella burnetii antigens].

PubMed (1994)

Tokarevich Nk Prosvernitsyn Sa Kartseva Na Kuzina Va

In this work the evaluation of macro- and micro-changes in the tissues of guinea pigs to the injection of inactivated antigens of C. burnetii is presented. The injection of these antigens in a dose of 50 micrograms induced only an insignificant reaction at the site of injection; in the brain of the animals, as well as in all examined internal organs, no visible pathological changes were present. During the first two weeks after the injection of the antigens signs of irritation of the reticuloendothelial system and hyperplasia of lymphoid tissue in T- and B-dependent zones were observed in the regional lymph nodes of the experimental animals. These changes became moderate by day 28.

Coxiella burnetii

Mononuclear phagocyte system

Q fever

Source

Cite

Citations (0)

Improvement of method of Coxiella burnetii detection in biological material based on groEL gene amplification

Zhurnal mikrobiologii epidemiologii i immunobiologii (2010)

Freĭlikhman Oa Yu. A. Panferova Tokarevich Nk

AIM Improvement of PCR for detection of Coxiella burnetii in field material by development of set of primers for amplification of groEL gene fragment. MATERIALS AND METHODS C. burnetii strains, samples from organs of wild rodents and laboratory animals, blood of healthy donors and laboratory animals. Methods of DNA isolation, PCR, and electrophoresis in agarose gel were used. RESULTS Nucleotide sequences of primers amplifying groEL gene fragment, which could increase specificity of PCR during work with field material were proposed. CONCLUSION Obtained data open perspective for using this method for detection of C. burnetii in environment.

Coxiella burnetii

Agarose gel electrophoresis

Q fever

Source

Cite

Citations (1)

[Quantitative evaluation of sensitization of lymphoid cells to normal tissue and bacterial antigens].

PubMed (1987)

Krylenkov Va Malygin Am Tokarevich Nk Red'ko Aa Dvaladze Na

Source

Cite

Citations (0)

Biological properties of chloroform/methanol extracts of Coxiella Burnetii.

PubMed (1993)

Tokarevich Nk Daĭter Ab Polotsky Ye M. Lukáčová J Kazár

Biological properties of antigens of whole cells of C. burnetii in phase I (WCI), their residue (CMR) and extract (CME) obtained by extraction of C. burnetti with chloroform/methanol mixture were subjected to a comparative study. It was shown that CME is able to react specifically with sera containing antibodies against WCI. The inoculation of animals with CME, but not with WCI and CMR does not cause changes of humoral and cell immunity to Coxiella and does not strengthen nonspecific antibacterial activity, but it can stimulate the formation of antibodies. Morphological changes of internal organs of mice were most expressed after the CMR inoculation and were practically absent after the CME inoculation.

Coxiella burnetii

Q fever

Rickettsiaceae

Source

Cite

Citations (2)

[The comparative characteristics of the immunogenic and immunomodulating activity of different antigenic preparations of the causative agent of Q fever Coxiella burnetii].

PubMed (1994)

Tokarevich Nk Pogodina On Freĭdlin Is Kuzina Va Kramskaia Ta

The immunogenicity of the Coxiella burnetii corpuscle antigen and two its components, obtained after the treatment with chloroform-methanol or extraction by trichloroacetic acid, was investigated. All the studied antigens were highly immunogenic. A strong correlation was discovered between the immunogenic and immunomodulating properties of the C. burnetii antigens. Cells of the mononuclear phagocytic system are the targets for stimulating action of the studied antigens. A cyclic increase of antibacterial activity and oxygen-dependent metabolism of the peritoneal cells was noted, in addition to the increase of the natural splenocyte cytotoxicity caused by the action of these antigens. The role of cytokines in rising the activity of peritoneal cells and splenocytes, caused by immunization of mice with the above antigenic substances, is discussed.

Coxiella burnetii

Q fever

Splenocyte

Source

Cite

Citations (1)

[Case of Q fever recurrence].

PubMed (1983)

Tokarevich Nk Udalova Gv Amosenkova Ni

Q fever

Source

Cite

Citations (0)

A study of possible transovarial and transphase transmission of borreliae by the tick Dermacentor reticulatus (Ixodidae)

Rudakova Sa Matusheko Ev Iakimenko Vv Tokarevich Nk Andreĭchuk IuV

A study of possible transovarial and transphase transmission of borreliae by the tick Dermacentor reticulatus is carried out. The possibility of borreliae transmission by the ticks of this species being infected spontaneously as well as experimentally is shown in principle. Although borreliae are preserved in the organism of D. reticulatus, low values of the infestation are established in the different stages of D. reticulatus development and in all steps of the study. D. reticulatus may be involved in the process of borreliae circulation in natural foci of tick-born borreliosis, but this species has no a significant importance for the maintenance of it.

Dermacentor reticulatus

Dermacentor

Transovarial transmission

Source

Cite

Citations (2)

[Determination of IgM- and IgG-antibodies to Coxiella burnetii].

PubMed (1979)

Tokarevich Nk

Coxiella burnetii

Q fever

Immunoglobulin M