Abstract [Background]The pqq genes which encodes phosphorus solubilizing from Bacillus mycoides Gnyt1 has been cloned and characterized. The role of this action on the function of pqq genes were detected. The phosphorus soluble pqq genes can secrete some specific organic activity acids to work but don’t destroy the living environment of plants such as soil. In contrast, this plants containing the pqq genes grew well, even better than other plants with lower phosphorus solubilization, in the phosphorus free medium even though its pqq genes activity and turned out phosphorus ring, while pqq dependent expression was induced.[Results]This reaserch that five pqq genes were cloned from Bacillus mycoides Gnyt1, phylogenetic analysis revealed that pBI- pqq A, pBI- pqq B, pBI- pqq C and pBI- pqq E similarly cluster with Phosphorus-soluble protein. Under the action of five promoters, each coding region of pqq A, pqq B, pqq C and pqq E from Bacillus mycoides Gnyt1 can directly participate in the phosphate solubilization of the strain, directly improving the phosphate solubilizing capacity of the plant, which have strong nitrogenase activity. Interestingly, changes in the phosphate solubilizing gene result in complete loss of the phosphate solubilization of the strain. However, the ability to dissolve the phosphorus ring and to dissolve the phosphorus was also exhibited in the medium having five phosphorus-dissolving genes.[Conclusions]The results indicated that the pqq genes were successfully cloned and a new specific phosphate-dissolving strain was formed. In this research, pqq genes were first cloned from Bacillus mycoides Gnyt1.
Abstract This study focused on the comparative genomic analyses of five carrying Bacillus strains which might give us insights on the difference and similarity in the genomic evolutionary kinships and contexts of Bacillus mycoides Gnyt1.Comparative genomic analyses of the carrying Bacillus strains genomes with emphasis on their antimicrobial resistance genes and virulence factors were performed.Most nitrogen-fixing genes and the phosphate-dissolving genes of the comparative genomic strain existed and the gene type was significantly different. And the action to nitrogen-fixing gene and phosphate-dissolving gene are dependent on different genes. Sharing some adherence, similarity, and the Bacillus mycoides strain Gnyt1 developed evolution is consistent with other Bacillus. Related functions absorb different gene-specific acquisitions, pigments, proteases, regulatory, secretory systems and functional factors associated with nitrogen-fixing phosphorus.Sharing most 16S rDNA analysis, Mauve-based genome-wide, Gene family analysis and phylogenetic tree, the Bacillus strain Gnyt1 and Bacillus mycoides strain AH621 differ in composition gene expression and expression cause conditions and factors.
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