FČ

Fedor Čiampor

Slovak Academy of Sciences

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i-10 index

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ZČ

Zuzana Čiamporová‐Zaťovičová

Slovak Academy of Sciences

Ján Kodada

Comenius University Bratislava

Manfred A. Jäch

Natural History Museum Vienna

J Rajcáni

Sabin Vaccine Institute

Michał Grabowski

University of Łódź

Torbjørn Ekrem

Norwegian University of Science and Technology

Tomasz Mamos

University of Łódź

E Závodská

Slovak Academy of Sciences

Florian Leese

University of Duisburg-Essen

Jana Bozáňová

Comenius University Bratislava

Cooperative Institutions

Slovak Academy of Sciences

155

Institute of Virology of the Slovak Academy of Sciences

Comenius University Bratislava

Centre National de la Recherche Scientifique

Czech Academy of Sciences

Biomedical Research Center of the Slovak Academy of Sciences

Uppsala University

Slovak Medical University

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

Institut de Biologia Evolutiva

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The tryptophan 41 is not essential for the function of the M2 proton channel of influenza A virus

International Congress Series (2004)

Tatiana Betáková Fedor Čiampor Alan Hay Eva Deakova

10.1016/j.ics.2004.01.028

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Citations (3)

Dental pulp mesenchymal stem/stromal cells labeled with iron sucrose release exosomes and cells applied intra-nasally migrate to intracerebral glioblastoma

Neoplasma (2016)

Ursula Altanerova Katarina Benejova Veronika Altanerová Silvia Tyčiaková Boris Rychlý

We report on a simple iron oxide (Venofer) labeling procedure of dental pulp mesenchymal stem cells (DP-MSCs) and DP-MSCs transduced with yeast cytosinedeaminase::uracilphosphoribosyltransferase (yCD::UPRT-DP-MSCs). Venofer is a drug approved for intravenous application to treat iron deficiency anemia in patients. Venofer labeling did not affect DP-MSCs or yCD::UPRT-DP-MSCs viability and growth kinetics. Electron microscopy of labeled cells showed internalized Venofer nanoparticles in endosomes. MRI relativity measurement of Venofer labeled DP-MSCs in a phantom arrangement revealed that 100 cells per 0.1 ml were still detectable. DP-MSCs or yCD::UPRT-DP-MSCs and the corresponding Venofer labeled cells release exosomes into conditional medium (CM). CM from yCD::UPRT-DP-MSCs in the presence of a prodrug 5-fluorocytosine caused tumor cell death in a dose dependent manner. Iron labeled DP-MSCs or yCD::UPRT-DP-MSCs sustained their tumor tropism in vivo; intra-nasally applied cells migrated and specifically engrafted orthotopic glioblastoma xenografts in rats.

10.4149/neo_2016_611

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Citations (34)

Analysis of specificity of interaction between synthetic key saccharide components of lipopolysaccharides and monoclonal antibodies to Coxiella burnetii.

PubMed (2005)

E Kováčová Dušan Cmarko V. Mucha R Toman Fedor Čiampor

Two monoclonal antibodies (MAbs) against the lipopolysaccharides (LPSs) of Coxiella burnetii (C.b.) strains Priscilla and Nine Mile were prepared characterized by their interaction with synthetic glycoconjugates representing parts of LPSs of C.b. in virulent phase. Both MAbs were directed against immunodominant epitopes comprising core constituent of LPSs, Kdo (3-deoxy-alpha-D-manno-2-octulo-pyranosylonic acid). ELISA showed that the anti-Nine Mile MAb 4/11 bound preferably to disaccharides (alpha-Kdo (2 --> 4) alpha-Kdo and alpha-Kdo (2 --> 4) alpha-(5d) Kdo), while the anti-Priscilla MAb 1/4/H bound to all conjugates, though with various intensity. On the other hand, immunoelectron microscopy revealed a positive binding of only one glycoconjugate, namely the trisaccharide alpha-Kdo (2 --> 4) alpha-Kdo (2 --> 4) alpha-Kdo-BSA, to both MAbs. In competitive ELISA (cELISA), the anti-Priscilla MAb 1/4/H distinguished the strains Nine Mile and Priscilla, while the anti Nine Mile MAb 4/11 did not.

Coxiella burnetii

Glycoconjugate

Immunoelectron microscopy

Q fever

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Citations (2)

Ultrastructural localization of influenza virus antigens in infected chick embryo cells as revealed by a peroxidase-labelled antibody method

Archives of Virology (1974)

Fedor Čiampor M Bystrická J Rajcáni

Budding

Cell membrane

10.1007/bf01240076

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Citations (4)

The tale of springs and streams: how different aquatic ecosystems impacted the mtDNA population structure of two riffle beetles in the Western Carpathians

PeerJ (2020)

Jana Bozáňová Zuzana Čiamporová‐Zaťovičová Fedor Čiampor Tomasz Mamos Michał Grabowski

The Western Carpathians are a particularly interesting part of the Carpathian Arc. According to recent molecular data upon aquatic and terrestrial taxa, this mountain area is an important biodiversity hotspot of Europe. Moreover, the W Carpathians include rich systems of karst springs inhabited by specific fauna, where molecular diversity and phylogeographic patterns are yet to be fully explored. Our study aims to compare population genetic structure and molecular diversity of two related and commonly co-occurring riffle beetles, Elmis aenea (PWJ Müller, 1806) and Limnius perrisi (Dufour, 1843) in the springs and streams of the W Carpathians using the mitochondrial DNA barcoding fragment of the cytochrome c oxidase subunit I gene (COI). The relatively stable thermal and chemical conditions of springs throughout unfavourable climatic settings make these highly specific lotic systems potentially ideal for a long-term survival of some aquatic biota. Populations of both elmid species were relatively homogeneous genetically, with a single dominant haplotype. However, we revealed that E. aenea significantly dominated in the springs, while L. perrisi preferred streams. Relative isolation of the springs and their stable conditions were reflected in significantly higher molecular diversity of the E. aenea population in comparison to L. perrisi . The results of Bayesian Skyline Plot analysis also indicated the exceptional position of springs regarding maintaining the population size of E. aenea . On the other hand, it seems that streams in the W Carpathians provide more effective dispersal channels for L. perrisi , whose population expanded much earlier compared to E. aenea . Present study points out that different demographic histories of these two closely related elmid species are manifested by their different habitat preference and molecular diversity.

DNA Barcoding

Dytiscidae

10.7717/peerj.10039

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Citations (10)

The European Reference Genome Atlas: piloting a decentralised approach to equitable biodiversity genomics

bioRxiv (Cold Spring Harbor Laboratory) (2023)

Ann M. Mc Cartney Giulio Formenti Alice Mouton Diego De Panis Luísa S Marins

A global genome database of all of Earth's species diversity could be a treasure trove of scientific discoveries. However, regardless of the major advances in genome sequencing technologies, only a tiny fraction of species have genomic information available. To contribute to a more complete planetary genomic database, scientists and institutions across the world have united under the Earth BioGenome Project (EBP), which plans to sequence and assemble high-quality reference genomes for all ~1.5 million recognized eukaryotic species through a stepwise phased approach. As the initiative transitions into Phase II, where 150,000 species are to be sequenced in just four years, worldwide participation in the project will be fundamental to success. As the European node of the EBP, the European Reference Genome Atlas (ERGA) seeks to implement a new decentralised, accessible, equitable and inclusive model for producing high-quality reference genomes, which will inform EBP as it scales. To embark on this mission, ERGA launched a Pilot Project to establish a network across Europe to develop and test the first infrastructure of its kind for the coordinated and distributed reference genome production on 98 European eukaryotic species from sample providers across 34 European countries. Here we outline the process and challenges faced during the development of a pilot infrastructure for the production of reference genome resources, and explore the effectiveness of this approach in terms of high-quality reference genome production, considering also equity and inclusion. The outcomes and lessons learned during this pilot provide a solid foundation for ERGA while offering key learnings to other transnational and national genomic resource projects.

10.1101/2023.09.25.559365

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Citations (9)

Carbonic anhydrase IX reduces E-cadherin-mediated adhesion of MDCK cells via interaction with β-catenin

Experimental Cell Research (2003)

Eliška Švastová Norbert Žilka Miriam Zaťovičová Adriana Gibadulinová Fedor Čiampor

10.1016/s0014-4827(03)00351-3

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Citations (236)

Ictelmis, a new riffle beetle genus from Ecuador (Coleoptera: Elmidae)

Zootaxa (2019)

Fedor Čiampor Marek Linský Zuzana Čiamporová‐Zaťovičová

A new minute riffle beetle genus Ictelmis gen. nov. and the type species Ictelmis martae sp. nov. are described from Ecuador. The description is supported by characteristic morphological features and DNA barcoding data, drawings and habitus photographs are provided. Based on molecular data and morphology it is suggested that Ictelmis is closely related with Onychelmis Hinton, and Notelmis Hinton.

Riffle

DNA Barcoding

Morphology

10.11646/zootaxa.4695.5.5

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Citations (64)

Characterization of RKZ isolate of ovine herpesvirus 1.

PubMed (2000)

Juraj Kopáček Ol'ga Rejholcová D. Koptidesová Fedor Čiampor F M Rijsewijk

Cytopathic effect (CPE) characterized mainly by foci of rounded cells was observed in cultures of primary plexus choroideus cells from healthy lamb following cryopreservation. It was possible to transmit the infectious agent to other primary cells of ovine origin by co-cultivation with infected cells. By indirect immunofluorescence microscopy it was found that high percentage of sheep (65-80% in 3 different herds from Slovakia) are infected with this infectious agent. Electron microscopy of cells with CPE revealed the presence of herpesvirus particles. Viral DNA was isolated from infected cells using pulse-field gel electrophoresis and further used as probe in Southern blot analysis. The probe reacted specifically only with DNA from cells infected with Ovine herpesvirus 1 (OvHV-1) but not with DNA of other ruminant herpesviruses. Some of the HindIII restriction fragments of DNA of the obtained OvHV-1 isolate denominated RKZ were cloned. Part of the H9 clone was sequenced identifying a gene that encoded a polypeptide homologous to conserved herpesvirus VP23 structural protein. From comparison of the sequence of this clone with VP23 sequences of other herpesviruses it was deduced that OvHV-1 might be classified within the Rhadinovirus genus of the Gammaherpesvirinae subfamily. The sequencing of the H9 clone of DNA of RKZ isolate enabled establishment of sensitive and highly specific polymerase chain reaction (PCR) assay for detection of OvHV-1.

clone (Java method)

Southern blot

Amplicon

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Citations (1)

Various methods of staining tissue cultures in electron microscopy.

PubMed (1970)

Fedor Čiampor M V Nermut B Holec

Negative stain

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Citations (0)