Charlotte Nielsen Agergaard

Odense University Hospital

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Co-Authors

Ulrik Stenz Justesen

Odense University Hospital

Michael Kemp

Odense University Hospital

Marianne Nielsine Skov

Odense University Hospital

Anders Koch

Statens Serum Institut

Marianne Antonius Jakobsen

657 Oslo

Kasper Klein

University of Southern Denmark

Jenny Dahl Knudsen

Rigshospitalet

Rudi Steffensen

Aalborg University Hospital

Julie May Jensen

Odense University Hospital

Kaspar René Nielsen

Aalborg University Hospital

Cooperative Institutions

Statens Serum Institut

University of Copenhagen

Copenhagen University Hospital

Odense University Hospital

University of Southern Denmark

bioMérieux (France)

Hvidovre Hospital

Rigshospitalet

Czech Academy of Sciences, Institute of Microbiology

Inserm

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Contaminated dicloxacillin capsules as the source of an NDM-5/OXA-48-producing Enterobacter hormaechei ST79 outbreak, Denmark and Iceland, 2022 and 2023

Eurosurveillance (2023)

Charlotte Nielsen Agergaard Lone Jannok Porsbo Thomas Vognbjerg Sydenham S. Hansen Kat Steinke

From October 2022 through January 2023, nine patients with NDM-5/OXA-48-carbapenemase-producing Enterobacter hormaechei ST79 were detected in Denmark and subsequently one patient in Iceland. There were no nosocomial links between patients, but they had all been treated with dicloxacillin capsules. An NDM-5/OXA-48-carbapenemase-producing E. hormaechei ST79, identical to patient isolates, was cultured from the surface of dicloxacillin capsules in Denmark, strongly implicating them as the source of the outbreak. Special attention is required to detect the outbreak strain in the microbiology laboratory.

Dicloxacillin

Strain (injury)

Penicillin resistance

10.2807/1560-7917.es.2023.28.9.2300108

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Citations (2)

A nationwide analytical and clinical evaluation of 44 rapid antigen tests for SARS-CoV-2 compared to RT-qPCR

Journal of Clinical Virology (2022)

Uffe Vest Schneider Maria Wendelboe Forsberg Thomas Daell Leineweber Christel Barker Jensen Khaled Ghathian

The SARS-CoV-2 pandemic has resulted in massive testing by Rapid Antigen Tests (RAT) without solid independent data regarding clinical performance being available. Thus, decision on purchase of a specific RAT may rely on manufacturer-provided data and limited peer-reviewed data.This study consists of two parts. In the retrospective analytical part, 33 RAT from 25 manufacturers were compared to RT-PCR on 100 negative and 204 positive deep oropharyngeal cavity samples divided into four groups based on RT-PCR Cq levels. In the prospective clinical part, nearly 200 individuals positive for SARS-CoV-2 and nearly 200 individuals negative for SARS-CoV-2 by routine RT-PCR testing were retested within 72 h for each of 44 included RAT from 26 manufacturers applying RT-PCR as the reference method.The overall analytical sensitivity differed significantly between the 33 included RAT; from 2.5% (95% CI 0.5-4.8) to 42% (95% CI 35-49). All RAT presented analytical specificities of 100%. Likewise, the overall clinical sensitivity varied significantly between the 44 included RAT; from 2.5% (95% CI 0.5-4.8) to 94% (95% CI 91-97). All RAT presented clinical specificities between 98 and 100%.The study presents analytical as well as clinical performance data for 44 commercially available RAT compared to the same RT-PCR test. The study enables identification of individual RAT that has significantly higher sensitivity than other included RAT and may aid decision makers in selecting between the included RAT.The study was funded by a participant fee for each test and the Danish Regions.

10.1016/j.jcv.2022.105214

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Citations (7)

False-Positive Diagnostics of Bordetella Pertussis using IS481 PCR is Limited in Danish Patients

The Open Microbiology Journal (2019)

Silje Vermedal Hoegh Charlotte Nielsen Agergaard Marianne Nielsine Skov Michael Kemp

Background: Bordetella pertussis is routinely detected using real-time PCR based on the multicopy insertion sequence IS 481 , which is not specific for Bordetella pertussis . Objective: The aim of this retrospective study was to evaluate the proportion of other Bordetella species misidentified as Bordetella pertussis using IS 481 -targeted real-time PCR. Methods: Clinical specimens from 228 Danish patients (median age 15 years, 0 to 90 years old) formerly identified as positive for Bordetella pertussis (IS 481 +) by routine PCR in 2011-2015, were subjected to real-time PCR targeting the insertion sequences IS 1002 and IS 1001 . Results: The results showed that 2.3% of the samples were false-positive for Bordetella pertussis . Conclusion: In conclusion, we found that misidentification of Bordetella pertussis using IS 481 PCR is limited in Danish patients.

Danish

Bordetella

10.2174/1874285801913010051

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Citations (1)

Draft Genome Sequence of Parabacteroides goldsteinii with Putative Novel Metallo-β-Lactamases Isolated from a Blood Culture from a Human Patient

Genome Announcements (2015)

Thøger Jensen Krogh Charlotte Nielsen Agergaard Jakob Møller‐Jensen Ulrik Stenz Justesen

Parabacteroides goldsteinii was isolated from a blood culture. Genomic DNA was sequenced using a MiSeq sequencer and assembled using the SPAdes genome assembler. The draft genome sequence was 6,851,868 bp, spanning 282 contigs of 5,253 coding sequences, 66 tRNAs, and 5 rRNAs. Several putative novel metallo-β-lactamases were discovered.

Sequence assembly

Sequence (biology)

genomic DNA

Coding region

10.1128/genomea.00937-15

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Citations (4)

Species identification of Streptococcus bovis group isolates causing bacteremia: a comparison of two MALDI-TOF MS systems

Diagnostic Microbiology and Infectious Disease (2017)

Charlotte Nielsen Agergaard Elisa Knudsen Rimtas Dargis Xiaohui Chen Nielsen Jens Jørgen Christensen

Streptococcus bovis

Bacteremia

Identification

10.1016/j.diagmicrobio.2017.02.007

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Citations (10)

Infantile Hemophagocytic Lymphohistiocytosis in a Case of Chediak-Higashi Syndrome Caused by a Mutation in the LYST/CHS1 Gene Presenting With Delayed Umbilical Cord Detachment and Diarrhea

Journal of Pediatric Hematology/Oncology (2014)

Christian Nielsen Charlotte Nielsen Agergaard Marianne Antonius Jakobsen Michael Møller Niels Fisker

A 2-month-old female infant, born to consanguineous parents, presented with infections in skin and upper respiratory tract. She was notable for delayed umbilical cord detachment, partial albinism, and neurological irritability. Giant granules were present in white blood cells. The intracellular perforin content in CD8 T cells seems to correlate to the immune activation state of the patient with 82% and 8% perforin-containing CD8 T cells at active and nonactive hemophagocytic lymphohistiocytosis (HLH) disease, respectively. HLH was confirmed by hemophagocytosis in bone marrow and absent natural killer cell activity. The patient carried a homozygous G>A mutation in the 3' splice site of intron 24 of the LYST/CHS1 gene, leading to the use of an alternative YAG splice site located in exon 25, introducing a premature STOP codon (L2355fsX2370; NP_000072.2). The early-onset accelerated phase in this severe phenotype of Chediak-Higashi syndrome was probably induced by rotaviral infection. Interestingly, the intracellular perforin content in CD8 T cells seems to correlate to the immune activation state of the patient. Late separation of the umbilical cord in concordance with clinical symptoms should lead to evaluation of a possible neutrophil dysfunction including Chediak-Higashi syndrome before onset of HLH.

Hemophagocytic Lymphohistiocytosis

Chédiak–Higashi syndrome

10.1097/mph.0000000000000300

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Citations (7)

Tre måneder gammelt spædbarn med Chediak-Higashi syndrom præsenteret i den acceleredrede fase

Christian Nielsen Charlotte Nielsen Agergaard Marianne Antonius Jakobsen Niels Fisker Torben Barington

Chédiak–Higashi syndrome

Source

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New tick-borne encephalitis virus hot spot in Northern Zealand, Denmark, October 2019

Eurosurveillance (2019)

Charlotte Nielsen Agergaard Maiken Worsøe Rosenstierne René Bødker Morten Rasmussen Peter Henrik Andersen

During summer 2019, three patients residing by Tisvilde Hegn, Denmark were hospitalised with tick-borne encephalitis (TBE) after tick bites. A new TBE virus (TBEV) micro-focus was identified in tick nymphs collected around a playground in Tisvilde Hegn forest. Estimated TBEV prevalence was 8%, higher than in endemic areas around Europe. Whole genome sequencing showed clustering to a TBEV strain from Norway. This is the second time TBEV is found in Ixodes ricinus outside Bornholm, Denmark.

Tick-borne encephalitis virus

Tick-borne encephalitis

10.2807/1560-7917.es.2019.24.43.1900639

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Citations (23)

Providing blood culture results at the appropriate time for the clinicians

Charlotte Nielsen Agergaard Elisa Knudsen Dorthe Strandgård Thorup Michael Kemp Ulrik Stenz Justesen

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A rapid multiplex pneumonia panel has the potential to improve antibiotic stewardship in hospitalised patients with suspected community-acquired pneumonia

Charlotte Nielsen Agergaard Marianne Nielsine Skov Thøger Gorm Jensen Michael Kemp

Antimicrobial Stewardship

Stewardship

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