Roxana Mihaela Filimon

National Institute for Research and Development in Forestry "Marin Drăcea"

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V. R. Filimon

University of Agricultural Sciences, Dharwad

Ancuța Nechita

University of Agricultural Sciences, Dharwad

Doina Damian

Liliana Rotaru

"Ion Ionescu de la Brad" Iasi University of Life Sciences

Rodica Paşa

National Institute of Research and Development for Biological Sciences

Florin Dumitru Bora

University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca

Simona Dunca

Alexandru Ioan Cuza University

Claudiu Ioan Bunea

University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca

Antoanela Patraș

"Ion Ionescu de la Brad" Iasi University of Life Sciences

Cintia Colibaba

"Ion Ionescu de la Brad" Iasi University of Life Sciences

Cooperative Institutions

University of Agricultural Sciences, Dharwad

"Ion Ionescu de la Brad" Iasi University of Life Sciences

Romanian Academy

Horticultural Research Institute

University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca

University of Bucharest

ORCID

National Institute of Research and Development for Biological Sciences

Academy of Romanian Scientists

Universidade Estadual Paulista (Unesp)

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"Studies on the behavior of a new wine grape hybrid elite in the climatic conditions of the Copou-Iasi viticultural center "

Romanian journal of Horticulture (2021)

Roxana Mihaela Filimon Doina Damin Ancuța Nechita V. R. Filimon

Climatic factors of the ecosystem directly influence the vegetative development of the grapevine, the production obtained, and its quality.Obtaining new genotypes with high ecological plasticity, superior yield and quality, and biological resistance can be achieved by continuously improving the existing assortment of grapevine cultivars.The crossbred of the valuable genitors and the selection of perspective genotypes is a permanent concern of horticultural researchers, in order to satisfy the consumer increasingly selective requirements.The purpose of the current study was to evaluate the adaptation of a new wine hybrid elite ('H.E.13.1.6')obtained at Research and Development Station for Viticulture and Oenology Iasi to the environmental conditions of the Copou-Iasi viticultural centre (NE of Romania), based on agrobiological and technological observations, and analysis on grape yield and wine quality.The hybrid elite showed high productions (up to 19 t/ha), with significant accumulations of sugars in grapes (>200 g/L) and high resistance to cryptogamic diseases, indicating a good adaptation to the environmental factors of the growing area.

Pillar

Adaptability

10.51258/rjh.2021.17

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Methods for producing low-alcohol wine I. Viticultural and pre-fermentation strategies

V. R. Filimon Ş. Tudose Sandu-Ville Florin Dumitru Bora Georgeta Tudor Roxana Mihaela Filimon

Ethanol Fermentation

Fermentation in winemaking

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THE PRODUCTION OF LOW-ALCOHOL WINES BY SEQUENTIAL SEMI-ANAEROBIC MUST FERMENTATION

Journal of Applied Life Sciences and Environment (2023)

V. R. Filimon Roxana Mihaela Filimon Ancuța Nechita Florin Dumitru Bora Liliana Rotaru

Nowadays, consumers go towards products that provide food security and nutritional richness, consumption of highly alcoholic beverages not complying with these health-related requirements. This study aimed to obtain low-alcohol wines by performing sequential alcoholic fermentation of grape must (Muscat Ottonel). Thus, 11 commercial yeast strains (10 Saccharomyces cerevisiae and 1 Torulaspora delbrueckii) were preliminarily tested in anaerobic and semi-anaerobic conditions. Based on laboratory tests, grape must fermentation was sequentially initiated in semi-anaerobic conditions (loose cotton plugs; intermittent homogenization), with the T. delbrueckii strain (106 CFU/mL) and the addition of preliminarily selected S. cerevisiae strains (104 CFU/mL), to the consumption of 50% of the sugars in the must, to complete the fermentation. By applying this technology, dry wines were obtained with an alcohol content lower by up to 1.10% vol., but with a lower concentration of volatile terpenes, due to additional must oxidation. Also, semi-anaerobiosis enhanced glycerol synthesis by yeasts (< 35%), with a positive impact on the sensory characteristics of the wine.

Torulaspora delbrueckii

Ethanol Fermentation

Fermentation in winemaking

10.46909/alse-554073

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Studies regarding pollen viability and germination capacity of some Vitis vinifera L. varieties

Roxana Mihaela Filimon V. R. Filimon Rodica Paşa

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New Malolactic Bacteria Strains Isolated from Wine Microbiota: Characterization and Technological Properties

Fermentation (2022)

V. R. Filimon Claudiu Ioan Bunea Ancuța Nechita Florin Dumitru Bora Simona Dunca

Malolactic fermentation (MLF) or biological decrease of wine acidity is defined as the enzymatic bioconversion of malic acid in lactic acid, a process performed by lactic acid bacteria (LAB). The procedures for the isolation of new indigenous LAB strains from the red wines produced in Copou Iasi wine center (NE of Romania) undergoing spontaneous malolactic fermentation, resulted in the obtaining of 67 catalase-negative and Gram-positive LAB strains. After testing in the malolactic fermentative process, application of specific screening procedures and identification (API 50 CH), two bacterial strains belonging to the species Oenococcus oeni (strain 13-7) and Lactobacillus plantarum (strain R1-1) with high yield of malolactic bioconversion, non-producing biogenic amines, and with active extracellular enzymes related to wine aroma, were retained and characterized. Tested in synthetic medium (MRS-TJ) for 10 days, the new isolated LAB strains metabolized over 98% of the malic acid at ethanol concentrations between 10 and 14 % (v/v), low pH (>3.0), total SO2 doses up to 70 mg/L and temperatures between 15 and 35 °C, showing high potential for future use in the winemaking process as bacterial starter cultures, in order to obtain high quality wines with increased typicity.

Oenococcus oeni

Bioconversion

Winemaking

Wine fault

Lees

Lactobacillus reuteri

Malic acid

Acetoin

Lactobacillus brevis

10.3390/fermentation8010031

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Citations (7)

Effects of exogenous growth regulators on agrobiological, technological and physiological characteristics of an interspecific grapevine cultivar

Biological Agriculture & Horticulture (2022)

Roxana Mihaela Filimon Liliana Rotaru V. R. Filimon

The use of interspecific grapevine cultivars offers new perspectives for sustainable viticulture. Although the application of exogenous plant hormones has become widespread in recent years, data regarding the response of interspecific grapevine cultivars to treatments with biostimulants are scarce. The aim of the study was to evaluate the effect of different doses of gibberellic acid (GA3) (25, 50 and 100 mg L−1), applied by spraying the inflorescences in the late flowering phenophase, and of the complex product Cropmax® (1.0, 2.5 and 5.0 mL L−1) applied by spraying the leaves in three different phenophases, on the phenology, fertility, physiological characteristics, grape yield and quality of the interspecific table grape cultivar Moldova (Guzal kara × Seyve-Villard 12-375), growing in temperate climate conditions (N-E Romania). The application of GA3 at 50 mg L−1 increased the plant productivity (36%), promoted fruit growth and rachis elongation, improved the percentage of the marketable yield (by 5.5%), balanced the sugar/acidity ratio, but affected the synthesis of phenolic compounds. Foliar application of Cropmax® at 2.5 mL L−1 significantly improved the berry diameter, and sugar and anthocyanin accumulation, but grape acidity remained high. All treatments allowed the normal development of plant phenophases and grape maturation, but GA3 application slightly extended the number of days of the annual biological cycle of the Moldova hybrid cultivar (up to 5.5 days), probably due to a temporary variation in the physiological parameters (except for stomatal conductance), as determined in mature leaves after the application of the exogenous growth regulator.

Gibberellic acid

Wine grape

Table grape

Vineyard

10.1080/01448765.2022.2126328

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Physico-Chemical Characterization, Phenolic Compound Extraction and Biological Activity of Grapevine (Vitis vinifera L.) Canes

Horticulturae (2023)

V. R. Filimon Claudiu Ioan Bunea Florin Dumitru Bora Roxana Mihaela Filimon Simona Dunca

Annual grapevine pruning produces large amounts of unused waste as woody canes. The current study is aimed at the sustainable valorization of viticultural waste by establishing phenolic compound extraction conditions, composition, and biological potential of crude and purified cane extracts of three Vitis vinifera L. cultivars growing in temperate climate conditions. Grapevine canes proved to be rich in carbohydrates and minerals; chlorophyll and carotenoids were also quantified. The highest yield of phenolic compounds was obtained when dry canes (<0.5 mm) were subjected to liquid–solid extraction (1:20 w/v) with 70% (v/v) ethanol, for 4 h at 35 °C, after a preliminary ultrasound treatment (6 min., 42 KHz); Pinot Gris canes showing the highest concentration of flavonoids and non-flavonoids. Stilbenes (resveratrol) and flavan-3-ols (catechin and epicatechin) were the main phenolic representative, resveratrol concentrations varying significantly between red-black (419.01–425.60 μg/g d.w.) and white (282.19 ± 4.14 μg/g d.w.) grape cultivars. Purified extracts (C-18 cartridge) exhibited higher antioxidant and antiradical activities compared to the crude extracts, and a higher antimicrobial effect, especially against Gram-positive (Staphylococcus aureus) pathogenic bacteria. Furthermore, Saccharomyces cerevisiae and Oenococcus oeni showed a reduced susceptibility even at high extract concentrations (>100 mg/mL). These findings indicate that grapevine canes represent a valuable source of natural bioactive compounds, that are currently insufficiently known and not exploited to their true functional and economic potential.

10.3390/horticulturae9111164

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Grape quality and ornamental potential of interspecific cultivars for temperate climate vineyards

The Journal of Horticultural Science and Biotechnology (2019)

V. R. Filimon Roxana Mihaela Filimon Antoanela Patraș Liliana Rotaru

Interspecific grapevine cultivars are the crossing product of two or more Vitis species. Due to the lack of information regarding the technological potential and quality characteristics of interspecific cultivars created in the last decades, the aim of this work was to evaluate the physico-chemical characteristics and physio-morphological particularities of grapes and leaves of three interspecific cultivars ('Purpuriu', 'Radames' and 'Moldova'), highly demanded for planting in the temperate climate area, having the hybrid cultivar 'Villard blanc' as common genitor. Cultivars reached grape maturity in mid to late September, forming red to dark red-seeded grapes. Ripe grapes showed a balanced sugar/acid ratio, a high content of nitrogenous compounds and good antioxidant activity due to rich polyphenolic content. Ripe berries showed high resistance to mechanical deformations, proving an increased resistance to postharvest handling. Moreover, leaf shape and size and their pigment composition indicated a high ornamental potential of the studied cultivars. These research findings contribute to a better understanding of the economic, nutritional and ornamental potential of valuable grapevine hybrids and hence their value for further breeding programs.

Ornamental plant

10.1080/14620316.2019.1631127

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Citations (5)

Nanotubes Biologically Active in Media with High Salt Concentration

Biotechnology & Biotechnological Equipment (2009)

Simona Merciu Corina Văcăroiu Roxana Mihaela Filimon Gabriela Popescu Silviu Preda

The current work focuses on the effect of silica and titania nanotubes and their synthesis conditions on the potential antibacterial activity. Since most investigations are oriented towards carbon nanotubes, we focused on studying the biological activity of oxide nanotubes obtained under different synthesis and different thermal treatment conditions. The antibacterial activity against Bacillus sphericus, Bacillus subtilis, Escherichia coli and Virgibacillus halodenitrificans were examined by the plate counting method. The biological activity was evaluated as dehydrogenase activity. The results revealed that titania nanotubes exhibit a higher antibacterial activity as compared with silica. This activity could be correlated with differences in the structure and morphology of these two types of nanotubes investigated.

10.1080/13102818.2009.10818550

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PRELIMINARY STUDIES ON SOME MORPHO-STRUCTURAL AND BIOCHEMICAL CHARACTERISATION OF SOME GENOTYPES OF Vitis vinifera L. CULTIVATED IN NORTHEAST ROMANIA

Journal of Applied Life Sciences and Environment (2024)

Liliana Rotaru V. R. Filimon Roxana Mihaela Filimon M. Mustea R. R. Bernardis

The monitoring of new grapevine varieties with superior agrobiological and technological characteristics, in relation to the evolution of the climatic factors, represents an important and continuous objective of the worldwide viticultural research and breeding programs. Observations and determinations of the current study were performed on 11 new table grapes varieties created in Romania, growing in the Ampelographic collection of the University of Life Sciences Iasi, north-eastern area of Romania. The grapevine varieties were evaluated under the morpho-structural aspect, regarding the leaf area, the average weight of the grapes, the number and weight of the berries, rachis weight, the number and weight of the seeds, the weight of the skin and pulp, calculating the main technological indices. From a biochemical point of view, the content of photosynthetic pigments in leaves, the concentration of soluble dry solids and titratable acidity of the grapes were determined in the climatic condition of the Copou - Iasi vineyard. Therefore, the monitoring of genetic resources provides useful data for grape producers and researchers regarding the integration of new table grape varieties into the viticultural ecosystems, evaluating their yield and quality in correlation with the evolution and influence of the climatic factors.

Morpho

10.46909/alse-571124

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Citations (1)