Event Abstract Back to Event Development of an on-line simulation platform for neuroscience research Hidetoshi Ikeno1*, Tadashi Yamazaki2, Yoshimi Kamiyama3, Akito Ishihara4, Keiichiro Inagaki5, Yutaka Hirata5, Yoshihiro Okumura6, Takayuki Kannon6, Shunji Satoh2, Hiroaki Wagatsuma7, Yoshiyuki Asai8, Yoko Yamaguchi6 and Shiro Usui9 1 University of Hyogo, Japan 2 The University of Electoro-Communications, Japan 3 Aichi Prefectural University, Japan 4 Chukyo University, Japan 5 Chubu University, Japan 6 RIKEN, Japan 7 Kyushu Institute of Technology, Japan 8 Okinawa Institute of Science and Technology, Japan 9 Toyohashi University of Technology, Japan Various data analysis algorithms and computational models have been proposed and utilized in neuroscience. Especially, theoretical approach to the neuronal system is indispensable for further advance in neuroscience. Recently, a number of software packages for data analysis and simulation of computational models have been provided on the Internet, and many of them are becoming open-access for researchers. However, we should to prepare the suitable computer environment where the numbers of software are installed for using these software resources. It will be sometimes caused problems by difference of operating system, version of software or lack of library files. In our project, we have been developing and providing a virtual machine environment for neuroscience research called Simulation Platform (Sim-PF, http://sim.neuroinf.jp) (Yamazaki et al., 2011) to use computational model and programs on the Internet without any barrier. The virtual machine based Linux is started by user demand, then executed script for downloading model file and running it on the virtual machine (VM) automatically. The server system managing VM is developed by Guacamole 0.8.4 and OpenNebula 4.12. Now, we are setting up for providing VM with Microsoft Windows OS. It is providing standard application software in neuroscience research, such as Fiji, vaa3D for image processing and 3D viewing; NEURON and GENESIS are for neuronal simulation. The Sim-PF provides not only utilities for visualization and simulation, but also the high-speed access to the registered data for retrieving and using large amount of data. It lets users be free from preparing the environment to carry out simulation, and lets users immediately get started on simulations for reviewing and analyzing existing models as well as for reviewing newly presented models submitted for publications. Up to now we have been developing online simulation pages for ModelDB contents, and the most of models registered on the ModelDB can be run on the web browser without installing any simulation software. As some of ModelDB contents are developing on MATLAB, one wishes to use these contents have to buy MATLAB license even just for evaluation. However, in the Sim-PF, these contents are registered after pre-compiling by MATLAB compiler and executed them for evaluation very easily. The platforms running by NIJC (http://www.neuroinf.jp/) supply various kinds of contents, such as data analysis program and experimental data. We are developing new contents, which can easily review these valuable resources on our platform. As an example of collaboration contents with other platform, users can review reconstruction process and results of neuronal morphological structure from confocal image data registered on the invertebrate brain platform (IVB-PF) on the Sim-PF. Registration of neuron model into the standard brain can be understand by following our contents. The tutorial movies for supporting users and education of students are making and uploading onto the Youtube. These are used to conduct a tutorial for newly joined students to the laboratory. In this moment, these movies are developed only in Japanese, but we are translating and uploading in English near future. The Sim-PF will be improved their functions and contents for further support education and research in neuroscience fields. Figure 1 References Yamazaki, T., Ikeno, H., Okumura, Y., Satoh, S., Kamiyama, Y., Hirata, Y., Inagaki, K., Ishihara, A., Kannon, T., Usui, S. (2011). Simulation Platform: a cloud-based online simulation environment. Neural Netw. 2011 Sep;24(7):693-8. doi: 10.1016/j.neunet.2011.06.010. Epub 2011 Jun 23. Keywords: simulation models, data visualization, Portal site, computational modeling, data analysis Conference: Neuroinformatics 2015, Cairns, Australia, 20 Aug - 22 Aug, 2015. Presentation Type: Demo, not to be considered for oral presentation Topic: Infrastructural and portal services Citation: Ikeno H, Yamazaki T, Kamiyama Y, Ishihara A, Inagaki K, Hirata Y, Okumura Y, Kannon T, Satoh S, Wagatsuma H, Asai Y, Yamaguchi Y and Usui S (2015). Development of an on-line simulation platform for neuroscience research. Front. Neurosci. Conference Abstract: Neuroinformatics 2015. doi: 10.3389/conf.fnins.2015.91.00029 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 07 Apr 2015; Published Online: 05 Aug 2015. * Correspondence: Prof. Hidetoshi Ikeno, University of Hyogo, Himeji, Japan, ikeno@shse.u-hyogo.ac.jp Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Hidetoshi Ikeno Tadashi Yamazaki Yoshimi Kamiyama Akito Ishihara Keiichiro Inagaki Yutaka Hirata Yoshihiro Okumura Takayuki Kannon Shunji Satoh Hiroaki Wagatsuma Yoshiyuki Asai Yoko Yamaguchi Shiro Usui Google Hidetoshi Ikeno Tadashi Yamazaki Yoshimi Kamiyama Akito Ishihara Keiichiro Inagaki Yutaka Hirata Yoshihiro Okumura Takayuki Kannon Shunji Satoh Hiroaki Wagatsuma Yoshiyuki Asai Yoko Yamaguchi Shiro Usui Google Scholar Hidetoshi Ikeno Tadashi Yamazaki Yoshimi Kamiyama Akito Ishihara Keiichiro Inagaki Yutaka Hirata Yoshihiro Okumura Takayuki Kannon Shunji Satoh Hiroaki Wagatsuma Yoshiyuki Asai Yoko Yamaguchi Shiro Usui PubMed Hidetoshi Ikeno Tadashi Yamazaki Yoshimi Kamiyama Akito Ishihara Keiichiro Inagaki Yutaka Hirata Yoshihiro Okumura Takayuki Kannon Shunji Satoh Hiroaki Wagatsuma Yoshiyuki Asai Yoko Yamaguchi Shiro Usui Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. 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Rhesus (Rh) glycoproteins are a family of membrane proteins capable of transporting ammonia. We isolated the full-length cDNA of a novel Rh glycoprotein, Rhp2, from a kidney cDNA library from the banded hound shark, Triakis scyllium. Molecular cloning and characterization indicated that Rhp2 consists of 476 amino acid residues and has 12 putative transmembrane spans, consistent with the structure of other family members. The shark Rhp2 gene was found to consist of only one coding exon. Northern blotting and in situ hybridization revealed that Rhp2 mRNA is exclusively expressed in the renal tubules of the sinus zone but not in the bundle zone and renal corpuscles. Immunohistochemical staining with a specific antiserum showed that Rhp2 is localized in the basolateral membranes of renal tubule cells. Double fluorescence labeling with phalloidin or labeling of the Na(+)/K(+)-ATPase further narrowed the location to the second and fourth loops in the sinus zone. Vacuolar type H(+)-ATPase was localized in apical membranes of the Rhp2-expressing tubule cells. Quantitative real-time PCR analysis and Western blotting showed that expression of Rhp2 was increased in response to elevation of environmental salinity. Functional analysis using the Xenopus oocyte expression system showed that Rhp2 has transport activity for methylammonium, an analog of ammonia. This transport activity was inhibited by NH(4)Cl but not trimethylamine-N-oxide and urea. These results suggested that Rhp2 is involved in ammonia reabsorption in the kidney of the elasmobranch group of cartilaginous fish comprising the sharks and rays.
It has been reported that the young people of today tend to engage in sexual behavior at an early age and the abortion rate is high. The purpose of this study was to investigate the attitudes of post-adolescents with regard to sexual behaviors and the relationship to self-identity and self-esteem.The subjects were 234 males (mean +/- SD 20.2 +/- 1.1 years) and 460 females (mean +/- SD 19.5 +/- 1.1 years) 4-year university students aged 18 to 23 years in Aichi Prefecture. An anonymous, self-report questionnaire was used to survey the subjects with regard to self-identity ("Establishment of Self" scale), self-esteem (Rosenberg Self-Esteem Scale), and sexual attitude and behavior. The scores on both scales and subscales of the "Establishment of Self" scale, "Foundation of Identity (Foundation)" and "Establishment of Identity (Establishment)", were calculated and intercompared, along with sexual attitude and behavior, controlled for age and school type.The mean total score of the "Establishment of Self" scale for males was 55.3 (SD9.2) and for females 52.2 (SD9.3), while those for the Rosenberg Self-Esteem Scale were 27.2 (SD5.5) and 25.7 (SD5.2), respectively. There was significant positive correlation all scales in both sexes. Both male and female students had positive attitude towards sex and a negative view of "traditional gender roles" particularly females. Of the subjects, 82.4% of males and 69.5% of females were thinking of accepting a request for sexual intercourse from their partners. Many students understood the "need for contraception", that is a component of contraceptive behavior. Most of them, however, didn't acquire the other components. A total of 68.3% of males and 48.2% of females had experienced sexual intercourse during the last year. Of these, 50.6% of males and 58.2% of females consistently used contraception. The score on the "Establishment of Self" scale was higher among both the male and female students who responded positively to having "communication ability," "learning ability," and "acquisition ability." Significant differences were found in total and "Establishment" scales among males, and all scales among females. The score on the Rosenberg Self-Esteem Scale was significantly higher with "learning ability" among males, and with "communication ability" and "learning ability" among females. However, no significant relationship was found between actual contraceptive behavior and any of the scales.In dealing with contraception, a gap exists between university students' attitudes and actual behavior. The scores on the "Establishment of Self" scale and the Rosenberg Self-Esteem Scale were found to be significantly higher for those who exhibited higher values for components of contraceptive behavior.
Varying and contending models have been proposed to explain how teleost ionocytes facilitate ion uptake from freshwater environments. A recently characterized member of the clc Cl − channel family ( clc2c ) has been proposed as a conduit for basolateral Cl − transport by Na + /Cl − cotransporter ( ncc )‐expressing ionocytes of stenohaline zebrafish. It is unknown to what extent clc2c is expressed in gill of euryhaline species, such as Mozambique tilapia, and whether expression is modulated in response to extrinsic (salinity) and intrinsic (hormones) factors. Here, we investigated prolactin (Prl) and local osmotic control of clc2c mRNA levels. First, branchial clc2c expression was markedly enhanced in freshwater‐acclimated tilapia compared with seawater‐acclimated tilapia. To investigate endocrine control of this salinity‐dependent expression, we injected hypophysectomized tilapia with ovine Prl. Ovine Prl induced a >8000‐fold increase in clc2c expression from saline‐injected controls. To then test whether Prl regulates clc2c expression in a gill‐autonomous fashion, we incubated gill filaments for 24 hours in the presence of homologous Prls, Prl 177 and Prl 188 . By 24 hours, Prl 188 stimulated clc2c expression ~5‐fold from controls. We then incubated filaments in media ranging from 280 to 450 mOsm/kg for 3 hours to investigate the possibility that extracellular osmolality exerts a local effect on clc2c expression. clc2c showed higher expression with decreasing media osmolalities. Collectively, our results suggest that both hormonal and extracellular osmotic conditions direct clc2c expression in branchial ionocytes. We propose that multifactorial modulation of clc2c expression contributes to the excellent adaptability of Mozambique tilapia to variations in environmental salinity. Support or Funding Information Supported by Start‐Up Funds (Skidmore College) to J.P.B.
