Julia Fernández-Rodrı́guez

University of Gothenburg

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Ruijin Shao

Shanghai Chest Hospital

Håkan Billig

Guangzhou Medical University

Tommy Nilsson

European Astronaut Centre

Birgitta Weijdegård

University of Gothenburg

Emil Egecioglu

Novo Nordisk (Denmark)

Johanna Bischof

European Molecular Biology Laboratory

Caterina Strambio‐De‐Castillia

University of Massachusetts Chan Medical School

Marı́a Páez de la Cadena

Universidade de Vigo

Arne Seitz

École Polytechnique Fédérale de Lausanne

Francisco Javier Rodrı́guez-Berrocal

Universidade de Vigo

Cooperative Institutions

University of Gothenburg

110

Sahlgrenska University Hospital

European Molecular Biology Organization

McGill University

Centre National de la Recherche Scientifique

Inserm

University of Wisconsin–Madison

Karolinska Institutet

Heidelberg University

University of Pisa

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Nuclear progesterone receptor A and B isoforms in mouse fallopian tube and uterus: implications for expression, regulation, and cellular function

AJP Endocrinology and Metabolism (2006)

Ruijin Shao Birgitta Weijdegård Karin Ljungström Anders Friberg Changlian Zhu

Progesterone and its interaction with nuclear progesterone receptors (PR) PR-A and PR-B play a critical role in the regulation of female reproductive function in all mammals. However, our knowledge of the regulation and possible cellular function of PR protein isoforms in the fallopian tube and uterus in vivo is still very limited. In the present study, we revealed that equine chorionic gonadotropin (eCG) treatment resulted in a time-dependent increase in expression of both isoforms, reaching a maximal level at 48 h in the fallopian tube. Regulation of PR-A protein expression paralleled that of PR-B protein expression. However, in the uterus PR-B protein levels increased and peaked earlier than PR-A protein levels after eCG treatment. With prolonged exposure to eCG, PR-B protein levels decreased, whereas PR-A protein levels continued to increase. Furthermore, subsequent treatment with human (h)CG decreased the levels of PR protein isoforms in both tissues in parallel with increased endogenous serum progesterone levels. To further elucidate whether progesterone regulates PR protein isoforms, we demonstrated that a time-dependent treatment with progesterone (P(4)) decreased the expression of PR protein isoforms in both tissues, whereas decreases in p27, cyclin D(2), and proliferating cell nuclear antigen protein levels were observed only in the uterus. To define the potential PR-mediated effects on apoptosis, we demonstrated that the PR antagonist treatment increased the levels of PR protein isoforms, induced mitochondrial-associated apoptosis, and decreased in epidermal growth factor (EGF) and EGF receptor protein expression in both tissues. Interestingly, immunohistochemistry indicated that the induction of apoptosis by PR antagonists was predominant in the epithelium, whereas increase in PR protein expression was observed in stromal cells of both tissues. Taken together, these observations suggest that 1) the tissue-specific and hormonal regulation of PR isoform expression in mouse fallopian tube and uterus, where they are potentially involved in regulation of mitochondrial-mediated apoptosis depending on the cellular compartment; and 2) a possible interaction between functional PR protein and growth factor signaling may have a coordinated role for regulating apoptotic process in both tissues in vivo.

Progesterone receptor

10.1152/ajpendo.00582.2005

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Citations (50)

Frontiers in Biomaterials Science (2024)

Pavitra Sokke Rudraiah Rafael Camacho Julia Fernández-Rodrı́guez Dror Fixler Jan Grimm

Correlated Multimodal Imaging (CMI) gathers information about the same specimen with two or more modalities that–combined–create a composite and complementary view of the sample (including insights into structure, function, dynamics and molecular composition). CMI allows one to reach beyond what is possible with a single modality and describe biomedical processes within their overall spatio-temporal context and gain a mechanistic understanding of cells, tissues, and organisms in health and disease by untangling their molecular mechanisms within their native environment. The field of CMI has grown substantially over the last decade and previously unanswerable biological questions have been solved by applying novel CMI workflows. To disseminate these workflows and comprehensively share the scattered knowledge present within the CMI community, an initiative was started to bring together imaging, image analysis, and biomedical scientists and work towards an open community that promotes and disseminates the field of CMI. This community project was funded for the last 4 years by an EU COST Action called COMULIS (COrrelated MUltimodal imaging in the LIfe Sciences). In this review we share some of the showcases and lessons learnt from the action. We also briefly look ahead at how we anticipate building on this initial initiative.

Modalities

Modality (human–computer interaction)

10.3389/fbiom.2024.1338115

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Estrogen-Induced Up-Regulation of Androgen Receptor (AR) Expression and Enhancement of AR Nuclear Translocation in Mouse Fallopian Tubes in vivo

Ruijin Shao Karin Ljungström Birgitta Weijdegård Emil Egecioglu Julia Fernández-Rodrı́guez

Female mice lacking AR display alterations in ovarian and uterine function. However, the biology of AR in the fallopian tube is not fully understood. To gain an insight into potential roles of AR in this tissue, we demonstrated that eCG treatment increased AR expression in a time-dependent manner and subsequent treatment with hCG decreased AR expression in mouse fallopian tubes. This expression pattern was positively associated with 17beta-estradiol and testosterone levels in vivo. Immunohistochemical analysis of fallopian tube epithelial cells revealed that nuclear localization of AR increased in parallel with decreased AR in the cytoplasm following eCG treatment. Moreover, we found that treatment with flutamide upregulated AR expression in immature mice in association with a decrease in serum testosterone levels, whereas the same treatment resulted in downregulation of AR expression in gonadotropin-stimulated mice with concomitant decreases in serum 17beta-estradiol concentrations, suggesting that androgen differs from estrogen in the regulation of AR expression. Furthermore, we demonstrated that DES increased both AR protein expression and nuclear location over a 48-h time course. DHT had rapid effects, with induction of AR expression and translocation at 6 h after injection, but unlike DES it had prolonged efficacy. In addition, we provided direct in vivo evidence that nuclear protein interaction between AR and p21(Cip1), a previously reported AR-regulated gene, was enhanced by gonadotropin stimulation. To our knowledge, this study provides the first demonstration to illustrate that estrogen as a principal regulator may contribute to regulate and activate AR in the fallopian tubes in vivo.

Flutamide

Gonadotropin

Fallopian tube

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Estrogen-induced upregulation of AR expression and enhancement of AR nuclear translocation in mouse fallopian tubes in vivo

AJP Endocrinology and Metabolism (2006)

Ruijin Shao Karin Ljungström Birgitta Weijdegård Emil Egecioglu Julia Fernández-Rodrı́guez

Fallopian tube

10.1152/ajpendo.00350.2006

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Citations (31)

3D TEM and SEM-array tomography of Hailey-Hailey Disease human skin biopsies

BIO Web of Conferences (2024)

Massimo Micaroni P.D. Jens Berndtsson P.D. Rafael Camacho Erin Cocks Giulio Gualdi

10.1051/bioconf/202412919004

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Occult Breast Lesion Localization plus Sentinel Node Biopsy (SNOLL): Experience with 959 Patients at the European Institute of Oncology

Annals of Surgical Oncology (2007)

Simonetta Monti Viviana Galimberti Giuseppe Trifirò Concetta De Cicco Nicolas Peradze

Occult

Surgical oncology

Lumpectomy

10.1245/s10434-007-9452-2

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Citations (104)

SNARE proteins mediate fusion between cytosolic lipid droplets and are implicated in insulin sensitivity

Nature Cell Biology (2007)

Pontus Boström Linda Andersson Mikael Rutberg Jeanna Perman Ulf Lidberg

Lipid droplet

10.1038/ncb1648

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Citations (336)

Detoxified Haemophilus ducreyi cytolethal distending toxin and induction of toxin specific antibodies in the genital tract

Vaccine (2010)

Annika Lundqvist Julia Fernández-Rodrı́guez Karin Ahlman Teresa Lagergård

Haemophilus ducreyi

Cytolethal distending toxin

10.1016/j.vaccine.2010.06.032

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Citations (7)

Strategies for Multimodal Image Data Transformation to a Common Format for Cloud Integration and Visualization

BIO Web of Conferences (2024)

Rafael Camacho Jens Berndtsson Massimo Micaroni Kaiqiang Zhang Julia Fernández-Rodrı́guez

10.1051/bioconf/202412913019

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Citations (0)

Community-developed checklists for publishing images and image analyses

Nature Methods (2023)

Christopher Schmied Michael S. Nelson Sergiy Avilov Gert‐Jan Bakker Cristina Bertocchi

Images document scientific discoveries and are prevalent in modern biomedical research. Microscopy imaging in particular is currently undergoing rapid technological advancements. However, for scientists wishing to publish obtained images and image-analysis results, there are currently no unified guidelines for best practices. Consequently, microscopy images and image data in publications may be unclear or difficult to interpret. Here, we present community-developed checklists for preparing light microscopy images and describing image analyses for publications. These checklists offer authors, readers and publishers key recommendations for image formatting and annotation, color selection, data availability and reporting image-analysis workflows. The goal of our guidelines is to increase the clarity and reproducibility of image figures and thereby to heighten the quality and explanatory power of microscopy data.

Disk formatting

Publication

CLARITY

10.1038/s41592-023-01987-9

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Citations (36)