Vittorio Saggiomo

Wageningen University & Research

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Aldrik H. Velders

Wageningen University & Research

Anton Bunschoten

Wageningen University & Research

Ulrich Lüning

Kiel University

Sijbren Otto

University of Groningen

L. Reck

Kiel University

Jeremy K. M. Sanders

University of Cambridge

Lars Kool

Physique et Mécanique des Milieux Hétérogènes

Fijs W. B. van Leeuwen

Leiden University Medical Center

Jennifer M. Klein

Louisiana State University Health Sciences Center New Orleans

Laura M. I. Schijven

Wageningen University & Research

Cooperative Institutions

Wageningen University & Research

University of Groningen

Leiden University Medical Center

University of Cambridge

Leiden University

University of Castilla-La Mancha

Erasmus MC

Centre National de la Recherche Scientifique

University of Twente

Kiel University

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ESPressoscope: A small and powerful approach for in situ microscopy

PLoS ONE (2024)

Ethan Li Vittorio Saggiomo Wei Ouyang Manu Prakash Benedict Diederich

Microscopy is essential for detecting, identifying, analyzing, and measuring small objects. Access to modern microscopy equipment is crucial for scientific research, especially in the biomedical and analytical sciences. However, the high cost of equipment, limited availability of parts, and challenges associated with transporting equipment often limit the accessibility and operational capabilities of these tools, particularly in field sites and other remote or resource-limited settings. Thus, there is a need for affordable and accessible alternatives to traditional microscopy systems. We address this challenge by investigating the feasibility of using a simple microcontroller board not only as a portable and field-ready digital microscope, but furthermore as a versatile platform which can easily be adapted to a variety of imaging applications. By adding a few external components, we demonstrate that a low-cost ESP32 camera board can be used to build an autonomous in situ platform for digital time-lapse imaging of cells. Our prototype of this approach, which we call ESPressoscope, can be adapted to applications ranging from monitoring incubator cell cultures in the lab to observing ecological phenomena in the sea, and it can be adapted for other techniques such as microfluidics or spectrophotometry. Our prototype of the ESPressoscope concept achieves a low power consumption and small size, which makes it ideal for field research in environments and applications where microscopy was previously infeasible. Its Wi-Fi connectivity enables integration with external image processing and storage systems, including on cloud platforms when internet access is available. Finally, we present several web browser-based tools to help users operate and manage our prototype’s software. Our findings demonstrate the potential for low-cost, portable microscopy solutions to enable new and more accessible experiments for biological and analytical applications.

Digital Microfluidics

10.1371/journal.pone.0306654

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Step-by-step : A microfluidic (PDMS) staircase device for size sorting microparticles down to 25 µm using a 3D-printed mold

Aldrik H. Velders Lisette van Lieshout Erik A. T. Brienen Benedict Diederich Vittorio Saggiomo

Microparticles are ubiquitous and span from living matter to microplastics to inorganic materials. Their detection and identification must be more accessible and time efficient. Microfluidic devices can filter microparticles from liquids, but fabricating microfluidics with lateral resolutions of a few tens of microns is complex, lengthy, and outside the reach of most scientists researching microparticles. In this article, we show how to use height features in a channel instead of relying on lateral elements for separating particles. The height features can be as small as 25 µm, along the Z axis, using consumer-grade 3D printers. We show the potential of such microfluidic devices for size-sorting parasite eggs such as Schistosoma haematobium, microplastics, and zooplankton.

Digital Microfluidics

10.26434/chemrxiv-2023-fnhkr-v2

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Citations (2)

CCDC 847405: Experimental Crystal Structure Determination

The Cambridge Structural Database (2012)

Jennifer M. Klein Jack K. Clegg Vittorio Saggiomo L. Reck Ulrich Lüning

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

10.5517/ccxfsnr

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Gold and Silver Dichroic Nanocomposite in the Quest for 3D Printing the Lycurgus Cup

Lars Kool Floris Dekker Anton Bunschoten Glen-Jacob Smales Brian R. Pauw

<div>The Lycurgus cup is worldwide admired in particular because of its fascinating dichroic property. The cup presents a green colour when the observer and the light source are on the same side (reflection), and a deep red colour when the observer and the light source are at opposite sides (transmission). This peculiar effect, which has perplexed scientists for centuries, was discovered to be due to the presence of nanoparticles in the glass. In particular, to two different metallic nanoparticles: silver nanoparticles (AgNP) and gold nanoparticles (AuNP). Here we show how dichroic silver nanoparticles and gold nanoparticles can be embedded in a 3D printable material in order to reproduce the same dichroic effect of the original Lycurgus cup. <br></div>

Dichroic glass

Silver nanoparticle

Dichroic filter

10.26434/chemrxiv.9784406

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T‐Cup: A Cheap, Rapid, and Simple Home Device for Isothermal Nucleic Acid Amplification

Global Challenges (2021)

Aldrik H. Velders Michel Ossendrijver Bart J. F. Keijser Vittorio Saggiomo

Abstract During the SARS‐CoV2 pandemic, it has become clear that centralized testing suffers from multiple bottlenecks. Logistics, number of machines, and people available to run the diagnostic tests are limited. A solution to those bottlenecks would be a fully decentralized system, where people can test themselves at home and only report back the outcome of the test in a centralized database. Here a noninstrumental device capable of achieving isothermal conditions useful for detecting the SARS‐CoV2 RNA using loop mediated amplification (LAMP) tests is presented. This device, compared to others reported in literature or present on the market, is cheap, easy to produce and use, and has little impact on the environment. Using a simple aluminum coffee capsule, a phase change material, and a 3D printed holder, this device, when placed in boiling water, is able to maintain a temperature of 65 °C for 25 min, required for running the LAMP reaction. In principle, this device can be applied to any LAMP reaction, and hence employed for many different applications, and can be deployed in large quantities in short amount of time.

Isothermal process

10.1002/gch2.202100078

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Gold nanoparticles embedded in a polymer as a 3D-printable dichroic nanocomposite material

Beilstein Journal of Nanotechnology (2019)

Lars Kool Anton Bunschoten Aldrik H. Velders Vittorio Saggiomo

Background: Nanotechnology, even if unknowingly, has been used for millennia. The occurrence of shiny colors in pottery and glass made hundreds and thousand of years ago is due to the presence of nanoparticles in the fabrication of such ornaments. In the last decade, 3D printing has revolutionized fabrication and manufacturing processes, making it easier to produce, in a simple and fast way, 3D objects. Results: In this paper we show how to fabricate a 3D-printable nanocomposite composed of dichroic gold nanoparticles and a 3D-printable polymer. The minute amount of gold nanoparticles used for obtaining the dichroic effect does not influence the mechanical properties of the polymer nor its printability. Thus, the nanocomposite can be easily 3D-printed using a standard 3D printer and shows a purple color in transmission and a brownish color in reflection. Conclusion: This methodology can be used not only by artists, but also for studying the optical properties of nanoparticles or, for example, for the 3D fabrication of optical filters.

Dichroic glass

Ornaments

Dichroic filter

10.3762/bjnano.10.43

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Citations (26)

Open hardware is at the heart of device research

Device (2023)

Vittorio Saggiomo

10.1016/j.device.2023.100012

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CCDC 1021075: Experimental Crystal Structure Determination

The Cambridge Structural Database (2015)

Suzanne M. Jansze Vittorio Saggiomo Antonius T. M. Marcelis Martin Lutz Aldrik H. Velders

Crystal (programming language)

10.5517/cc138hxs

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HistoEnder: a 3D printer-based histological slide autostainer that retains 3D printer functions

Marco Ponzetti Ganga Chinna Rao Devarapu Nadia Rucci Armando Carlone Vittorio Saggiomo

Automated microscope slide stainers are usually very expensive and unless the laboratory performs heavy histological work it is difficult to justify buying a 2000-10000€ machine. As a result, histology and pathology labs around the world lose thousands of working hours for following procedures that could be easily automated. Herein, we propose a simple modification of an open-source 3D printer, the Creality Ender-3, into an automated microscope slide autostainer, the HistoEnder. The HistoEnder is cheap (less than 200€), modular, and easy to set up, with only two 3D-printed parts needed. Additionally, the 3D printer retains its full functionality, and it can be reverted back into 3D printing in less than 1 minute. The g-code associated with the procedure is extremely simple, and can be written by anyone. The HistoEnder can also be used in chemistry and material science laboratories for automating surface modifications and dip coating.

3d printer

Rapid Prototyping

3d printed

10.31224/osf.io/sjz4e

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Easy, On-Site Applicable DNA Extraction in Combination with Lamp Analysis for the Early Detection of DNA Derived from Processed Animal Proteins in Poultry Feed

Marleen M. Voorhuijzen Vittorio Saggiomo Valerie van der Vorst Toine F. H. Bovee Aldrik H. Velders

10.2139/ssrn.4982801

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