LAST YEAR (1) we reported the isolation of a protein, ‘metakentrin’ (2), from the hog pituitary gland, which repairs, maintains, and stimulates the interstitial d cells of the testes and ovaries and causes the formation of corpora lutea from preformed graafian follicles. This protein was shown to be homogeneous by ultracentrifugation and electrophoretic analysis and also to possess a constant solubility in one solvent. The view that the protein is the hormone could not be taken as proved. For the sake of simplicity, however, this protein has been referred to as a ‘hormone’ without any qualifications because there are no experimental results known to be in conflict with this view. In this paper we shall describe a simplified procedure for the preparation of the hormone together with experiments consistently supporting the belief that the protein is the hormone rather than an inactive protein on which an extremely active hormone is adsorbed.
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStudies in the Chlorophyll Series. XIV. Potentiometric Titration in Acetic Acid Solution of the Basic Groups in Chlorophyll Derivatives1J. B. Conant, B. F. Chow, and Emma M. DietzCite this: J. Am. Chem. Soc. 1934, 56, 10, 2185–2189Publication Date (Print):October 1, 1934Publication History Published online1 May 2002Published inissue 1 October 1934https://pubs.acs.org/doi/10.1021/ja01325a060https://doi.org/10.1021/ja01325a060research-articleACS PublicationsRequest reuse permissionsArticle Views98Altmetric-Citations18LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The erythropoietic stimulating principle present in the filtrate of plasma obtained from anemic rabbits was partially purified by ethanol fractionation. This material was active in increasing Fe59 intake of normal rats and in producing a reticulocytosis and hematocrit elevation in repeatedly injected normal rats. Fe59 uptake was stimulated by as little as 0.08 mg of the fraction representing a concentration of 3,000 fold over that occurring in the plasma.
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