Mingxing Chu

Chinese Academy of Agricultural Sciences

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Document Type

Co-Authors

Ran Di

Chinese Academy of Agricultural Sciences

121

Xiaoyun He

Chinese Academy of Agricultural Sciences

102

Xiangyu Wang

Chinese Academy of Agricultural Sciences

Qiuyue Liu

Chongqing University

Xiaosheng Zhang

University of Electronic Science and Technology of China

Wenping Hu

Chinese Academy of Agricultural Sciences

Xiaofei Guo

Chinese Academy of Agricultural Sciences

Jinlong Zhang

Guizhou University

G. L. Cao

Liaocheng University

Tao Feng

Beijing Academy of Agricultural and Forestry Sciences

Cooperative Institutions

Chinese Academy of Agricultural Sciences

259

Ministry of Agriculture and Rural Affairs

138

Chinese Academy of Sciences

120

Australia and New Zealand Banking Group

Institute of Animal Sciences

Anhui Agricultural University

Chinese Academy of Medical Sciences & Peking Union Medical College

Shanghai Jiao Tong University

Nanjing Medical University

China Agricultural University

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[Study on BMP15 and GDF9 as candidate genes for prolificacy of Small Tail Han sheep].

PubMed (2005)

Mingxing Chu Lin-Hua Sang Jinyu Wang Fang Li YE Su-cheng

Growth differentiation factor-9

Candidate gene

Source

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Citations (29)

Effects of RFRP-3 on reproductive function and energy balance in mammals

Hereditas (Beijing) (2012)

Wei Xiang Ping Lai Baoyun Zhang Ping-Qing Wang Mingxing Chu

The hypothalamo-pituitary-gonadal (HPG) axis integrates internal and external cues via a balance of stimulatory and inhibitory neurochemical systems to regulate reproductive function in mammals. However, RFRP-3 is a unique inhibitor of HPG axis at the hypothalamuic level in mammals to date. A large number of previous studies have confirmed that RFamide-related peptide (RFRP-3) suppresses gonadotropin-releasing hormone (GnRH) system and luteinizing hormone (LH) secretion, thereby affecting the reproduction. However, whether the inhibition of LH secretion by RFRP-3 occurs at the pituitary level or the hypothalamus level is not clear. It is interesting that RFRP-3 is also related to signal pathway of melatonin modulating mammal seasonal reproduction, but little is known about the effects of melatonin on the RFRP-3 neuron up to now. In addition, RFRP-3 also plays an important role in the regulation of energy balance and behavior. The regulatory mechanism of RFRP-3 in HPG axis and role of RFRP-3 in modulating mammalian energy balance, as well as behavior, are systematically elaborated and the remaining unsolved problems are also discussed in this paper.

Neurochemical

10.3724/sp.j.1005.2012.00969

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Citations (1)

Association of a Single Codon Deletion in Bone Morphogenetic Protein 15 Gene with Prolificacy in Small Tail Han Sheep

Asian-Australasian Journal of Animal Sciences (2004)

Wei Guo Mingxing Chu Xiaojie Deng J. D. Feng Ning Li

Small Tail Han Sheep has significant characteristics of high prolificacy and non-seasonal ovulatory activity and is an excellent local sheep breed in P. R. China.Recently a novel member of the transforming growth factor β (TGFβ) superfamily termed bone morphogenetic protein 15 (BMP15) was shown to be specifically expressed in oocytes and to be essential for female fertility.Therefore, BMP15 is a candidate gene for reproductive performance of Small Tail Han Sheep.The whole genomic nucleotide sequence of BMP15 gene in Small Tail Han Sheep was searched for polymorphisms by PCR-SSCP and direct sequencing, and only one polymorphism was found.The polymorphism was a result of a 3 base pair deletion, which eliminated a single Leu codon (CTT).The allelic frequencies for A (without deletion) and B (with a codon deletion) are 0.73 and 0.27 respectively.The effects of BMP15 genotype on litter size were evaluated using the least squares model.This indicated that there was a significant association between litter size of Small Tail Han Sheep and a deletion in BMP15 gene (p=0.02<0.05).Small Tail Han Sheep ewes with AA and AB genotype produce on average 0.5 and 0.3 more lambs per litter than those ewes with BB genotype.

Association (psychology)

10.5713/ajas.2004.1491

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Citations (5)

Integrated Hypothalamic Transcriptome Profiling Reveals the Reproductive Roles of mRNAs and miRNAs in Sheep

Frontiers in Genetics (2020)

Zhuangbiao Zhang Jishun Tang Ran Di Qiuyue Liu Xiangyu Wang

Early studies have provided a wealth of information on the functions of microRNAs, however, less is known regarding their functions in hypothalamus involved in sheep reproduction. To explore the potential roles of mRNAs and miRNAs in sheep (FecB++) hypothalamus, totally, 172 and 235 differentially expressed genes (DEGs) and 42 and 79 differentially expressed miRNAs (DEmiRNAs) were identified using RNA sequencing in polytocous sheep in the follicular phase versus monotocous sheep in the follicular phase (PF vs. MF), and polytocous sheep in the luteal phase versus monotocous sheep in the luteal phase (PL vs. ML), respectively. We also identified several key mRNAs (e.g., POMC, GNRH1, PRL, GH, TRH, and TTR) and mRNA-miRNAs pairs (e.g., TRH coagulated by oar-miR-379-5p, oar-miR-30b, oar-miR-152, oar-miR-495-3p, oar-miR-143, oar-miR-106b, oar-miR-218a, oar-miR-148a, and PRL regulated by oar-miR-432) through functional enrichment analysis, and the identified mRNAs and miRNAs may function through influencing GnRH activities or nerve cell survival associated with reproductive hormone release via direct or indirect ways. This study represents an integral analysis between mRNAs and miRNAs in sheep hypothalamus, and provide a valuable resource for elucidating sheep prolificacy.

Profiling (computer programming)

10.3389/fgene.2019.01296

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Citations (26)

Transcription factor PRDM15 binds to the promoter mutation locus of PPP2R5C promoted proliferation of goat granulosa cells

Research Square (Research Square) (2023)

Peng Wang Wentao Li Ziyi Liu Xiaoyun He Yufang Liu

Abstract Protein phosphatase 2 regulatory subunit B’gamma ( PPP2R5C ) is involved in a variety of physiological processes in mammals, such as growth and development, cellular metabolism, and immunity. Here, the PCR sequencing and KASP genotyping sequencing were used to detect the distribution of SNPs of PPP2R5C , and then a series of molecular biology technology were used to detect the regulation pathway between PPP2R5C and kidding number trait of goats. The sequencing results showed that there was a g.65977460A > G mutation locus in the promoter region of PPP2R5C, and there were 223 individuals with genotype GG, 91 with genotype GA, and 51 with genotype AA in the tested population of Yunshang black goats. Association analysis showed that the kidding number trait of second and third parity were significantly higher for the individuals with AA genotype than those with the AG and GG genotypes. Subsequently, the results of dual luciferase activity assay showed that the promoter activity of PPP2R5C -mutant-A was significantly higher than that of PPP2R5C -Wild-G ( P < 0.05). The transcription factor PRDM15 was predicted to bind to a mutation in PPP2R5C (g.65977460A > G). The results of ChIP-qPCR showed that the transcription factor PRDM15 could bind to the promoter region of PPP2R5C . Moreover, the expression of PPP2R5C , proliferation factors CDK4 and Cyclin-D2 in goat GCs was significantly increased after PRDM15 (PR/SET domain 15) overexpression ( P < 0.05). While the results of EdU and CCK-8 showed that the proliferation rate of goat GCs increased after PRDM15 overexpression and the proliferation efficiency of goat GCs was significantly reduced after PRDM15 inhibition ( P < 0.05).Finally, the expression of the PI3K-Akt pathway (phosphatidylinositol-3-kinase/protein kinase B) marker AKT2 (AKT serine/threonine kinase 2) was significantly increased in granulosa cells overexpressing PRDM15 , and the opposite was true after inhibition of PRDM15 . Together, our results showed that PPP2R5C g.65977460A > G was significantly associated with the kidding number in Yunshang black goats. The transcription factor PRDM15 was identified to bind to the promoter region of PPP2R5C g.65977460A > G mutation locus promoted the proliferation of goat granulosa cells (GCs) by the PPP2R5C /PI3K-Akt pathway. These results provide a new insight into the molecular mechanisms underlying the phenotypic changes in goats.

10.21203/rs.3.rs-2499551/v1

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Evolutionary relationship and population structure of domestic Bovidae animals based on MHC-linked and neutral autosomal microsatellite markers

Molecular Immunology (2020)

E Guangxin Lipeng Chen Dong‐Ke Zhou Bai‐Gao Yang Jiahua Zhang

Major histocompatibility complex (MHC) genes are critical for disease resistance or susceptibility responsible for host-pathogen interactions determined mainly by extensive polymorphisms in the MHC genes. Here, we examined the diversity and phylogenetic pattern of MHC haplotypes reconstructed using three MHC-linked microsatellite markers in 55 populations of five Bovidae species and compared them with those based on neutral autosomal microsatellite markers (NAMs). Three-hundred-and-forty MHC haplotypes were identified in 1453 Bovidae individuals, suggesting significantly higher polymorphism and heterozygosity compared with those based on NAMs. The ambitious boundaries in population differentiation (phylogenetic network, pairwise FST and STRUCTURE analyses) within and between species assessed using the MHC haplotypes were different from those revealed by NAMs associated closely with speciation, geographical distribution, domestication and management histories. In addition, the mean FST was significantly correlated negatively with the number of observed alleles (NA), observed (HO) and expected (HE) heterozygosity and polymorphism information content (PIC) (P < 0.05) in the MHC haplotype dataset while there was no correction of the mean FST estimates (P> 0.05) between the MHC haplotype and NAMs datasets. Analysis of molecular variance (AMOVA) revealed a lower percentage of total variance (PTV) between species/groups based on the MHC-linked microsatellites than NAMs. Therefore, it was inferred that individuals within populations accumulated as many MHC variants as possible to increase their heterozygosity and thus the survival rate of their affiliated populations and species, which eventually reduced population differentiation and thereby complicated their classification and phylogenetic relationship inference. In summary, host-pathogen coevolution and heterozygote advantage, rather than demographic history, act as key driving forces shaping the MHC diversity within the populations and determining the interspecific MHC diversity.

10.1016/j.molimm.2020.05.005

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Citations (5)

Progesterone receptor-mediated molecular mechanisms on mam-malian female reproduction

Hereditas (Beijing) (2012)

Yuehua Kang Baoyun Zhang Ping-Qing Wang Mingxing Chu Ping Chin Lai

The steroid hormone, progesterone, plays a critical role in regulation of mammalian female reproductive activities.Besides the non-genomic activity of progesterone on target cells, its main physiological effect is caused through genomic action by the ligand-dependent nuclear progesterone receptor.The genomic and non-genomic effects of progesterone collectively mediate various female reproductive functions, including ovulation, embryo implantation, maintenance of pregnancy, initiation of parturition, and development of mammary gland.Although a large number of candidate genes regulated by progesterone have been identified by gene chip technology, the traditional progesterone response elements located in the promoter region of downstream target genes havenot been detected.Accordingly, it was suggested thatthe mechanism of nuclear progesterone receptors regulating transcription may be different from other nuclear steroid receptors.In this review, we summarized the mechanisms of progesterone receptors mediating the physiological effects in various female re-1224 HEREDITAS (Beijing) 2012 第 34 卷 productive activities.

Progesterone receptor

Steroid hormone

Steroid hormone receptor

10.3724/sp.j.1005.2012.01223

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Citations (1)

A performance assessment methodology for low-level waste facilities

M Kozak Mingxing Chu P.A. Mattingly

A performance assessment methodology has been developed for use by the US Nuclear Regulatory Commission in evaluating license applications for low-level waste disposal facilities. This report provides a summary of background reports on the development of the methodology and an overview of the models and codes selected for the methodology. The overview includes discussions of the philosophy and structure of the methodology and a sequential procedure for applying the methodology. Discussions are provided of models and associated assumptions that are appropriate for each phase of the methodology, the goals of each phase, data required to implement the models, significant sources of uncertainty associated with each phase, and the computer codes used to implement the appropriate models. In addition, a sample demonstration of the methodology is presented for a simple conceptual model. 64 refs., 12 figs., 15 tabs.

10.2172/6724198

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Citations (18)

Genome‐wide association study of body weight and conformation traits in neonatal sheep

Animal Genetics (2020)

Lin Tao Xiaoyun He Linxiang Pan J. W. Wang Shangquan Gan

Sheep, an important source of meat, dairy products and wool, play an essential part in the global agricultural economy. Body weight and body conformation are key traits in the sheep industry; however, their underlying genetic mechanisms are poorly understood. In this study, a GWAS was implemented to identify promising genes possibly linked to birth weight (BW) and body conformation traits in neonatal sheep, using a high-throughput chip (630 K). After quality control, 277 individuals and 518 203 variants were analyzed using gemma software in a mixed linear model. A total of 48 genome-wide suggestive SNPs were obtained, of which four were associated with BW, four with withers height (WH), 11 with body length (BL) and 29 with chest girth (CG). In total, 39 genes associated with BW and body conformation traits were identified by aligning to the sheep genome (Ovis aries_v4.0), and most of them were involved in the cell cycle and body development. Promising candidate genes found included the following: FOS like 2 or AP-1 transcription factor subunit (FOSL2) for BW; potassium voltage-gated channel subfamily D member 2 (KCND2) for WH; transmembrane protein 117 (TMEM117), transforming growth factor beta induced (TGFBI), and leukocyte cell-derived chemotaxin 2 (LECT2) for BL; and trafficking kinesin protein 1 (TRAK1) and LOC101102529 for CG. These results provide cues for similar studies aiming at uncovering the genetic mechanisms underlying body development, and marker-assisted selection programs focusing on BW and body conformation traits in sheep.

Genome-wide Association Study

Withers

Candidate gene

10.1111/age.12904

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Citations (29)

Additional file 17 of Pineal gland transcriptomic profiling reveals the differential regulation of lncRNA and mRNA related to prolificacy in STH sheep with two FecB genotypes

Figshare (2021)

Chunyan Li Xiaoyun He Zijun Zhang Chunhuan Ren Mingxing Chu

Additional file 17: Supplementary material 7A. Total set of the top 20 KEGG enrichment pathways for differentially expressed lncRNA targets in MM_FP vs MM_LP. Supplementary material 7B. Total set of the top 20 KEGG enrichment pathways for differentially expressed lncRNA targets in MM_FP vs ww_FP. Supplementary material 7C. Total set of the top 20 KEGG enrichment pathways for differentially expressed lncRNA targets in MM_LP vs ww_LP. Supplementary material 7D. Total set of the top 20 KEGG enrichment pathways for differentially expressed lncRNA targets in ww_FP vs ww_LP.

10.6084/m9.figshare.14059290

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