Storage of human blood for six to eight weeks in ACD solution resulted in definite hemolysis which paralleled an increase in osmotic fragility determined by a new method. Mechanical injury as a result of delivering blood by airdrop markedly aggravated this hemolysis. Whole blood stored for six weeks in ACD solution with adenine added had less overt hemolysis, less osmotic fragility and better posttransfusion survival than blood from the same subject stored only in ACD solution. Comparison of changes in the osmotic fragility of stored red blood cells showed good correlation with in vivo survival measurements and suggested that this method of testing might be useful in programs to evaluate the effect of additives on the viability of banked blood.
Contemporary kidney preservation methods involve storing at 4 degree C up to 24 h prior to transplantation. By decreasing the storage temperature to below 0 degree C, we hypothesized that the safe storage time could be significantly lengthened.The efficacy of a proprietary CryoStasis (CrS) storage solution for the subzero preservation of kidneys was tested, with or without addition of a hyperactive insect antifreeze protein (TmAFP).Rat kidneys were stored in either University of Wisconsin (UW) solution (4 degree C, 24 h), CrS (-2 degree C, 48 h), or CrS with 61.5 µM TmAFP (-4.4 degree C, 72 h). Following storage, viability was assessed with MTT reduction assays and live vs. dead cell (FDA/PI) staining. Markers of ischemic damage were analyzed using fluormetric substrates for caspase-3 and calpain activity.Kidneys stored in CrS for 48 h and CrS with TmAFP for 72 h displayed similar levels of enzymatic activity compared to 24 h UW controls.This methodology shows promise to prolong the safe storage time of kidneys and offers the potential of increased organ availability for renal transplants.
Journal Article Increasing the Effectiveness and Utilization of Human Blood by Differential Storage Get access Turman E. Allen, MSC, USA, Turman E. Allen, MSC, USA MAJ. Blood Bank Section and Pathology Service, Walter Reed General Hospital, Walter Reed Army Medical Center, Washington, D. C. 20012 Search for other works by this author on: Oxford Academic Google Scholar John P. Kalas, MC, USA, John P. Kalas, MC, USA MAJ. Blood Bank Section and Pathology Service, Walter Reed General Hospital, Walter Reed Army Medical Center, Washington, D. C. 20012 Search for other works by this author on: Oxford Academic Google Scholar Joseph H. Keffer, MC, USAF Joseph H. Keffer, MC, USAF CAPT. Blood Bank Section and Pathology Service, Walter Reed General Hospital, Walter Reed Army Medical Center, Washington, D. C. 20012 Search for other works by this author on: Oxford Academic Google Scholar American Journal of Clinical Pathology, Volume 47, Issue 3_ts, March 1967, Pages 413–415, https://doi.org/10.1093/ajcp/47.3_ts.413 Published: 01 March 1967 Article history Received: 11 May 1966 Published: 01 March 1967
