Furio Gramatica

Don Carlo Gnocchi Foundation

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Marzia Bedoni

Don Carlo Gnocchi Foundation

Carlo Morasso

Istituti Clinici Scientifici Maugeri

Renzo Vanna

National Research Council

Dóra Méhn

Joint Research Centre

M. Casella

Center for Biomolecular Nanotechnologies

Silvia Picciolini

Don Carlo Gnocchi Foundation

Davide Prosperi

University of Milano-Bicocca

Fabio Ciceri

Vita-Salute San Raffaele University

Enzo Di Fabrizio

Polytechnic University of Turin

Roberta Carabalona

Don Carlo Gnocchi Foundation

Cooperative Institutions

Don Carlo Gnocchi Foundation

University of Milan

Istituti di Ricovero e Cura a Carattere Scientifico

University of Milano-Bicocca

University of Pavia

Politecnico di Milano

Università Cattolica del Sacro Cuore

Mylan (Switzerland)

International Flame Research Foundation

Scuola Superiore Sant'Anna

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

A microneedle-based transdermal drug delivery device for the treatment of chronic disease

M. Casella Marzia Bedoni Enzo Di Fabrizio Elena Donetti E. Maculan

Source

Cite

Citations (0)

Immobilised gold nanostars in a paper-based test system for surface-enhanced Raman spectroscopy

Vibrational Spectroscopy (2013)

Dóra Méhn Carlo Morasso Renzo Vanna Marzia Bedoni Davide Prosperi

Malachite green

Surface-Enhanced Raman Spectroscopy

10.1016/j.vibspec.2013.05.010

Cite

Citations (33)

Simultaneous detection of multiple biomarkers by means of SERS on polymer nanopillar gold arrays

Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE (2016)

Carlo Morasso Silvia Picciolini Dóra Méhn Paola Pellacani Ana Frangolho

The detection of biomarkers by means of Surface Enhanced Raman Spectroscopy (SERS) is foreseen to became a very important tool in the clinical practice because of its excellent sensitivity and potential for the simultaneous detection of multiple biomarkers. In the present paper we describe how it was possible to build a sensor for the detection of genetic biomarkers involved in acute myeloid leukemia. The assay is based on the use of a specifically designed SERS substrate made of a 2D crystal structure of polymeric pillars embedded in a gold layer. This substrate is characterized by good enhancing properties coupled with an excellent homogeneity. The SERS substrate was conjugated with DNA probes complementary to a target sequence and used in a sandwich assay with gold nanoparticles labeled with a second DNA probe and a Raman reporter. The so developed assay allowed the detection of a leukemia biomarker (WT1 gene) and an housekeeping gene with low picomolar sensitivity. At last, we optimized the assay in order to tackle one of the main limitations of SERS based assay: the loss of signal that is observed when the Raman spectra are collected in liquid. Combining a preferential functionalization on the polymeric pillars with a different height of the polymer pillars from the gold layer the assay demonstrated its effectiveness even when measured in buffer.

Surface-Enhanced Raman Spectroscopy

Nanopillar

10.1117/12.2208339

Cite

Citations (0)

Micropatterned non-invasive dry electrodes for Brain-Computer Interface

Furio Gramatica Roberta Carabalona M. Casella Cinzia Cepek Enzo Di Fabrizio

Partially or completely paralyzed patients can benefit from advanced neuro-prostheses in which a continuous recording of electroencephalogram (EEG) is required, operating some processing and classification to control a computer (BCI, brain-computer interfaces). Patients are so allowed to control external devices or to communicate simple messages through the computer, just concentrating their attention on codified movements or on a letter or icon on a digital keyboard. Conventional electrodes usually require skin preparation and application of electrolytic gel for high quality low amplitude biopotentials recordings and are not suitable for being easily used by patient or caregivers at home in BCI or equivalent systems. In this report we describe the fabrication and characterization of dry (gel not required), non-invasive, user-friendly biopotential electrodes. The electrodes consist of a bidimensional array of micro-needles designed to pierce the first dielectric skin layer (stratum corneum) and establishing a direct contact with the living and electrical conducting cells in the epidermis (no blood vessels and nerve terminations). The easy and immediate application of the spiked electrodes makes them also attractive for every surface long-term biosignal measurements, even at patient's home (EEG, electrocardiogram, etc).

Biosignal

Interface (matter)

10.1109/issmdbs.2006.360099

Cite

Citations (8)

Train with Me: A Study Comparing a Socially Assistive Robot and a Virtual Agent for a Rehabilitation Task

Lecture notes in computer science (2019)

Valentina Vasco Cesco Willemse Pauline Chevalier Davide De Tommaso Valerio Gower

Rehabilitation robotics

Telerehabilitation

10.1007/978-3-030-35888-4_42

Cite

Citations (17)

Proton-Ion Medical Machine Study (PIMMS), 2

P J Bryant L Weisser Luigi P. Badano G Borri M Crescenti

Source

Cite

Citations (4)

One-step synthesis of star-like gold nanoparticles for surface enhanced Raman spectroscopy

Materials Chemistry and Physics (2013)

Carlo Morasso Dóra Méhn Renzo Vanna Marzia Bedoni Elena Forvi

Surface-Enhanced Raman Spectroscopy

Suspension

10.1016/j.matchemphys.2013.11.024

Cite

Citations (25)

Preliminary technological assessment of microneedles-based dry electrodes for biopotential monitoring in clinical examinations

Sensors and Actuators A Physical (2012)

Elena Forvi Marzia Bedoni Roberta Carabalona Monica Soncini Paolo Mazzoleni

SIGNAL (programming language)

Clinical Practice

10.1016/j.sna.2012.04.019

Cite

Citations (83)

Interland4All – Using AR to Promote a Safe and Responsible Use of the Internet by Frail Children

Lecture notes in computer science (2024)

Mattia Randazzo Chiara Giovannini Valentina Di Giusto Anna Cavallini Mirta Michilli

10.1007/978-3-031-71713-0_18

Cite

Citations (0)

Raman spectroscopy for the assessment of acute myeloid leukemia: a proof of concept study

Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE (2014)

Renzo Vanna Cristina Tresoldi Paola Ronchi Aufried Lenferink Carlo Morasso

Acute myeloid leukemia (AML) is a proliferative neoplasm, that if not properly treated can rapidly cause a fatal outcome. The diagnosis of AML is challenging and the first diagnostic step is the count of the percentage of blasts (immature cells) in bone marrow and blood sample, and their morphological characterization. This evaluation is still performed manually with a bright field light microscope. Here we report results of a study applying Raman spectroscopy for analysis of samples from two patients affected by two AML subtypes characterized by a different maturation stage in the neutrophilic lineage. Ten representative cells per sample were selected and analyzed with high-resolution confocal Raman microscopy by scanning 64x64 (4096) points in a confocal layer through the volume of the whole cell. The average spectrum of each cell was then used to obtain a highly reproducible mean fingerprint of the two different AML subtypes. We demonstrate that Raman spectroscopy efficiently distinguishes these different AML subtypes. The molecular interpretation of the substantial differences between the subtypes is related to granulocytic enzymes (e.g. myeloperoxidase and cytochrome b₅₅₈), in agreement with different stages of maturation of the two considered AML subtypes . These results are promising for the development of a new, objective, automated and label-free Raman based methods for the diagnosis and first assessment of AML.

10.1117/12.2039186

Cite

Citations (3)