Saeideh Sadat Shobeiri

Sabzevar University of Medical Sciences

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Mojtaba Sankian

Mashhad University of Medical Sciences

Malihe Moghadam

Mashhad University of Medical Sciences

Saeid Abediankenari

Mazandaran University of Medical Sciences

Safoora Pordel

Mashhad University of Medical Sciences

Navideh Haghnavaz

Mashhad University of Medical Sciences

MohammadAli Rezaee

Kurdistan University of Medical Sciences

Bahareh Ansari

Mashhad University of Medical Sciences

Zahra Rahmani

Shahid Beheshti University of Medical Sciences

Mohammad Ali Rezaee

Kurdistan University of Medical Sciences

Mohammadreza Dashti

Mashhad University of Medical Sciences

Cooperative Institutions

Mashhad University of Medical Sciences

Mazandaran University of Medical Sciences

Shiraz University of Medical Sciences

Sabzevar University of Medical Sciences

Tehran University of Medical Sciences

Babol University of Medical Sciences

Shahid Beheshti University of Medical Sciences

Imam Khomeini Hospital

Shiraz University

University of Tehran

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Anti-inflammatory effects of the Portulaca oleracea hydroalcholic extract on human peripheral blood mononuclear cells

Medical Journal of the Islamic Republic of Iran (2018)

Esmaeil Allahmoradi Saeid Taghiloo Versa Omrani‐Nava Saeideh Sadat Shobeiri Mohsen Tehrani

Background:Portulaca oleracea, known as Purslane, is an annual growing herb with wide distribution around the world and traditionally used to manage several diseases. Different therapeutic properties as an anti-fever agent as well as anti-inflammatory and analgesic effects have been attributed to P. oleracea. The aim of this study was to investigate the effects of P. oleracea aerial extract on production of pro- and anti-inflammatory cytokines by human peripheral blood mononuclear cells (PBMCs). Methods: Aerial parts of P. oleracea (stems and leaves) were collected and extracted by percolation using methanol. The optimal and non-cytotoxic dose of hydro-alcoholic extract for cell culture analysis was determined by MTT assay. To assess the antiinflammatory effects of P. oleracea, PBMCs obtained from 12 normal volunteers were cultured in RPMI complete medium and cotreated with E. coli lipopolysaccharide (LPS) and P. oleracea hydro-alcoholic extract. Following 18-hour incubation, culture supernatants were harvested for measurement of secreted TNF-α, IL-6 and IL-10 by ELISA. Statistical analyses were performed using the SPSS v.20, and data analyzed by Kolmogorov-Smirnov, Mann-Whitney U, Kruskal-Wallis and post Hoc tests. P-values<0.05 were considered significant. Results: The optimal non-cytotoxic concentration of P. oleracea aerial extract was defined as 100 μg/ml based on MTT viability assay. P. oleracea hydro-alcoholic extract significantly decreased the concentration of both pro-inflammatory cytokines TNF-α and IL-6 in LPS-stimulated PBMCs (p<0.001 and p<0.001, respectively). However, the concentration of IL-10 as an anti-inflammatory cytokine, did not show any statistically significant change (p=0.390). Conclusion: Our findings highlighted the potential anti-inflammatory properties of P. oleracea in herbal medicine. Future analysis on different constituents of total extract may confirm its therapeutic effects as a promising anti-inflammatory compound.

Portulaca

MTT assay

10.14196/mjiri.32.80

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Citations (20)

In vitro identification of single-stranded DNA aptamers targeting human IL-23 using the protein-SELEX strategy

Journal of Pharmaceutical and Biomedical Analysis (2024)

Mohammad Ali Rezaee Saeideh Sadat Shobeiri Malihe Moghadam Kazem Mashayekhi Mojtaba Sankian

Aptamer

Identification

10.1016/j.jpba.2024.116245

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Citations (2)

Evaluation of NK cell level and HLA-G1 expression in peripheral blood in threatened-abortion

Tehran University Medical Journal TUMS Publications (2015)

Saeideh Sadat Shobeiri Saeid Abediankenari Zahra Rahmani Hossein Nataj Hadi Hossein Azadeh

Threatened abortion

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An epicutaneous therapeutic pollen-allergen extract delivery system in the allergic rhinitis mouse model: based on allergen loading on DC-specific aptamers conjugated nanogolds

Research Square (Research Square) (2023)

Safoora Pordel Navideh Haghnavaz MohammadAli Rezaee Saeideh Sadat Shobeiri Bahareh Ansari

Abstract Background : We investigated whether gold nanoparticles (GNPs) and dendritic cells (DC)-specific aptamer-modified GNPs (Apts-GNP) could be effective for epicutaneous immunotherapy (EPIT) in the case of pollen allergen extracts containing a variety of allergenic and nonallergenic components. Methods : BALB/c mice were sensitized to total protein extract of Platanus orientalis pollen and then epicutaneously treated in different groups either with free P. orientalis total pollen extract, naked GNPs, the total extract loaded GNPs, and the total extract loaded Apts-GNPs with and without skin-penetrating peptides (SPPs). We then examined the specific IgE level (sIgE), total IgE concentration (tIgE) in the serum sample, IL-4, IL-17a, IFN-γ, and IL-10 cytokine concentrations in re-stimulated splenocytes with the total extract and the mixture of its recombinant allergens, nasopharyngeal lavage fluid (NALF) analysis, and histopathological analysis of lung tissue. Results : The current study showed that the total extract-loaded GNPs, especially Pla. ext (50 μg)-GNPs, significantly decreased sIgE, tIgE, IL-17a, and IL-4 concentrations, immune cells and eosinophils infiltration in NALF, and increased IL-10 and IFN-γ concentrations compared with the PBS-treated group. In addition, histopathological analysis of lung tissue showed that it also led to a remarkable decrease in allergic rhinitis (AR)-associated inflammation and histopathological damage. The DC-targeted group showed greatest improvement in AR-related immune factors and had no histopathological damage compared with the same dose without aptamer. Conclusion : Consequently, it seems that loading total protein extract on the GNPs and the Apt-modified GNPs could be an effective approach to improve EPIT efficacy in pollen-induced AR.

10.21203/rs.3.rs-3456343/v1

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An epicutaneous therapeutic pollen-allergen extract delivery system in an allergic rhinitis mouse model: based on allergen loading on DC-specific aptamers conjugated nanogolds

Immunologic Research (2023)

Safoora Pordel Navideh Haghnavaz MohammadAli Rezaee Saeideh Sadat Shobeiri Bahareh Ansari

Gold nanoparticles (GNPs) have previously been suggested as appropriate carriers for allergen-specific immunotherapy (AIT). In this study, we assessed efficacy of GNPs and dendritic cells (DC)-specific aptamer-modified GNPs (Apts-GNP) for epicutaneous immunotherapy (EPIT) in the case of pollen allergen extracts containing a variety of allergenic and non-allergenic components. BALB/c mice were sensitized to the total protein extract of Platanus orientalis pollen and epicutaneously treated in different groups either with free P. orientalis total pollen extract, naked GNPs, total extract loaded GNPs, and total extract loaded Apts-GNPs with and without skin-penetrating peptides (SPPs). Then, the specific IgE level (sIgE), total IgE concentration (tIgE) in the serum sample, IL-4, IL-17a, IFN-γ, and IL-10 cytokine concentrations in re-stimulated splenocytes with the total extract and mixture of recombinant allergens, nasopharyngeal lavage fluid (NALF) analysis, and histopathological analysis of lung tissue were evaluated. This study indicated the total extract-loaded GNPs, especially Pla. ext (50 μg)-GNPs, significantly decreased sIgE, tIgE, IL-17a, and IL-4 concentrations, immune cells and eosinophils infiltration in NALF, and increased IL-10 and IFN-γ concentrations compared with the PBS-treated group. In addition, the histopathological analysis of lung tissue showed a significant decrease in allergic inflammation and histopathological damage. The DC-targeted group revealed the most significant improvement in allergic-related immune factors with no histopathological damage compared with the same dose without aptamer. Loading total protein extract on the GNPs and the Apt-modified GNPs could be an effective approach to improve EPIT efficacy in a pollen-induced allergic mouse model.

10.1007/s12026-023-09445-6

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Citations (4)

Anti-IL-17A ssDNA aptamer ameliorated psoriasis skin lesions in the imiquimod-induced psoriasis mouse model

International Immunopharmacology (2022)

Saeideh Sadat Shobeiri MohammadAli Rezaee Safoora Pordel Navideh Haghnnavaz Mohammadreza Dashti

Imiquimod

Aptamer

HaCaT

S100A8

10.1016/j.intimp.2022.108963

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