The purpose of this article is to illustrate the spectrum of central airway and vascular complications in lung transplantation using MDCT, with an emphasis on the usefulness of advanced postprocessing techniques.MDCT is an invaluable tool in the diagnosis, evaluation, and posttreatment assessment of central airway and vascular complications in lung transplant recipients. Advanced postprocessing techniques provide complementary information that is visually accessible and anatomically meaningful for the clinician.
Abstract Objective The aims of this feasibility study of an adapted lifestyle intervention for adults with lung cancer were to (1) determine rates of enrollment, attrition, and completion of 5 nurse‐patient contacts; (2) examine demographic characteristics of those more likely to enroll into the program; (3) determine acceptability of the intervention; and (4) identify patient preferences for the format of supplemental educational intervention materials. Methods This study used a single‐arm, pretest and posttest design. Feasibility was defined as ≥20% enrollment and a completion rate of 70% for 5 nurse‐patient contact sessions. Acceptability was defined as 80% of patients recommending the program to others. Data was collected through electronic data bases and phone interviews. Descriptive statistics, Fisher's exact test and Wilcoxon rank sum test were used for analyses. Results Of 147 eligible patients, 42 (28.6%) enrolled and of these, 32 (76.2%) started the intervention and 27 (N = 27/32; 84.4%; 95% CI, 67.2%‐94.7%) completed the intervention. Patients who were younger were more likely to enroll in the study ( P = .04) whereas there were no significant differences by gender ( P = .35). Twenty‐three of the 24 (95.8%) participants' contacted posttest recommended the intervention for others. Nearly equal numbers of participants chose the website (n = 16, 50%) vs print (n = 14, 44%). Conclusion The intervention was feasible and acceptable in patients with lung cancer. Recruitment rates were higher and completion rates were similar as compared to previous home‐based lifestyle interventions for patients with other types of cancer. Strategies to enhance recruitment of older adults are important for future research.
489 Bone marrow (BM) chimerism is a promising technique in the induction of donor-specific transplantation tolerance. The clinical application of BM chimerism, however, continues to be hampered by the morbidity and mortality associated with bone marrow transplantation, including Graft vs. Host Disease(GVHD) and engraftment failure. The Facilitating Cell (FC) is a unique marrow-derived cell population that promotes allogeneic stem cell (SC) engraftment without inducing GVHD. Recipients demonstrate donor-specific transplantation tolerance to solid organ and cellular grafts. The FC comprises 0.4% of the BM population, and has been phenotypically characterized and isolated via flow cytometric cell sorting as CD8+/CD3+/αβTCRdim/- andγδTCRdim/-. Although previously thought to require conventional TCR heterodimers for expression, functional CD3 complexes have been firmly established to occur on immature thymocytes in the flow cytometric absence of αβ- or γδTCR heterodimers. In all described cases, such CD3 expression is associated with TCRβ and a TCRα surrogate or chaperone protein. Therefore, the primary aim of this study was to determine if similar TCRβ-associated molecules were present on the FC cell surface. The FC population was isolated via multiparameter sterile live cell sorting from the bone marrow of B10.BR mice, using BM-derived T-cells(CD8+/CD3+/TCRbright) as controls. Surface protein biotinylation was performed, followed by immunoprecipitation with an anti-βTCR monoclonal antibody (H57-597). SDS-PAGE and Western blotting of the immunoprecipitate allowed visualization of the individual proteins associated with TCRβ. Such analysis demonstrated a 33kD protein on the FC(FCp33) that is not present on bone marrow-derived T-Cells[Figure]. This protein is similar in size to the Pre-T-Cell receptor alpha (pTα) expressed as part of the TCRβ-pTα heterodimer involved in regulation of T-Cell maturation in TCRα deficient mice. These findings suggest that a similar TCRβ-FCp33 complex may be important in regulating the ability of the FC to promote SC engraftment and survival, while establishing donor-specific transplantation tolerance.
