Minimum global macrofungal species diversity is expected around 49.500 worldwide [1]. Little of macrofungi species have been recently studied for bioactive metabolites [2]. These include polysaccharides, proteins, lipids together with a large diversity of secondary metabolites, i.e., polyphenols, terpenoids, alkaloids. Fungal metabolites represented potential therapeutic agents against many diseases as Alzheimer, diabetes, malaria, infectious diseases and cancer [3-6].We selected macrofungal species (Basidiomycota, Ascomycota) growingin the Mediterranean area to be screened for their biological properties of great interest. Macrofungi have been investigated for their chemical composition using various solvent polarities with extraction yield up than 30%. HPLC and TLC profiling have been performed. In parallel some of the crude extracts have been evaluated for their anti-microbial activity by disc diffusion method and for anti-proliferative activities in HCT116 colon adenocarcinoma cell line. Bioguided purifications of the bioactive extracts are in progress to identify promising therapeutic compounds.

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Hydrologie et bactéries antibiorésistantes. Récit et résultats d’une démarche pluridisciplinaire sur une rivière hyper-polluée du district d’Abidjan

Patricia Licznar‐Fajardo Jean Louis Perrin

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A new methodology to assess antimicrobial resistance of bacteria in coastal waters; pilot study in a Mediterranean hydrosystem

Comptes Rendus Géoscience (2017)

Ayad Almakki Kévin Esteves Audrey S. Vanhove Thomas Mosser Fabien Aujoulat

The global resistome of coastal waters has been less studied than that of other waters, including marine ones. Here we develop an original method for characterizing the antimicrobial resistance of bacterial communities in coastal waters. The method combines the determination of a new parameter, the community Inhibitory Concentration (c-IC) of antibiotics (ATBs), and the description of the taxonomic richness of the resistant bacteria. We test the method in a Mediterranean hydrosystem, in the Montpellier region, France. Three types of waters are analyzed: near coastal river waters (Lez), lagoon brackish waters (Mauguio), and lake freshwaters (Salagou). Bacterial communities are grown in vitro in various conditions of temperature, salinity, and ATB concentrations. From these experiments, we determine the concentrations of ATB that decrease the bacterial community abundance by 50% (c-IC50) and by 90% (c-IC90). In parallel, we determine the taxonomic repertory of the resistant growing bacteria communities (repertory of Operational Taxonomic Units [OTU]). Temperature and salinity influence the abundance of the cultivable bacteria in presence of ATBs and hence the c-ICs. Very low ATB concentrations can decrease the bacterial abundance significantly. Beside a few ubiquitous genera (Bacillus, Pseudomonas, Shewanella, Vibrio), most resistant OTUs are specific of a type of water. In brackish water, resistant OTUs are more diverse and their community structure less vulnerable to ATBs than those in freshwater. We anticipate that c-IC measurement combined with taxonomic description can be applied to any littoral region to characterize the resistant bacterial communities in the coastal waters. This would help us to evaluate the vulnerability of aquatic ecosystems to antimicrobial pressure.

10.1016/j.crte.2017.09.009

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Antibiorésistance dans les eaux naturelles de la zone urbaine de Montpellier

Fabien Aujoulat Estelle Jumas‐Bilak Patricia Licznar‐Fajardo

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Can a regulatory control network be used for a territorial monitoring of antimicrobial resistance in water intended for human consumption?

The Science of The Total Environment (2024)

Patricia Licznar‐Fajardo Lara Guillerez Isabelle Zorgniotti Agnès Masnou Marion Mimbielle

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10.1016/j.scitotenv.2024.176828

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