Eric D. Hoffer

Emory University

Author Statistics

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Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Christine Dunham

Emory University

Tatsuya Maehigashi

Emory University

Samuel Hong

The University of Texas MD Anderson Cancer Center

Scott M. Stagg

Florida State University

Atousa Mehrani

Salk Institute for Biological Studies

S. Sunita

Indian Veterinary Research Institute

Ha An Nguyen

Emory University

Seok Hoon Hong

Illinois Institute of Technology

Kenneth C. Keiler

The University of Texas at Austin

S. Subaramanian

Cooperative Institutions

Emory University

Microbiotix (United States)

National Institutes of Health

National Institute of Allergy and Infectious Diseases

Pennsylvania State University

Rutgers, The State University of New Jersey

Medicines for Malaria Venture

Kennesaw State University

Howard Hughes Medical Institute

Florida State University

Author Statistics

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Research Field

Sus scrofa heart L-lactate dehydrogenase ternary complex with NADH and oxamate

Eric D. Hoffer Brooke A. Andrews Christine Dunham R. Brian Dyer

Ternary complex

Malate dehydrogenase

10.2210/pdb6cep/pdb

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Citations (0)

Thermus thermophilus 70S complex containing 16S G299A ram mutation and empty A site.

Eric D. Hoffer Tatsuya Maehigashi C.E. Fagan Christine Dunham

Thermus thermophilus

10.2210/pdb6buw/pdbx

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Citations (0)

Modified ASL proline bound to Thermus thermophilus 70S (near-cognate)

Eric D. Hoffer Tatsuya Maehigashi S. Subaramanian Seok Hoon Hong Christine Dunham

Thermus thermophilus

Cognate

10.2210/pdb6nsh/pdb

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Citations (0)

Structure of trans-translation inhibitor bound to E. coli 70S ribosome with P site tRNA

Eric D. Hoffer Atousa Mehrani Kenneth C. Keiler Scott M. Stagg Christine Dunham

T arm

A-site

10.2210/pdb6om6/pdb

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Citations (0)

Role of RNA Modifications in tRNA Structural Stability and Accurate Protein Synthesis

Biophysical Journal (2020)

Christine Dunham Eric D. Hoffer Ha An Nguyen Sunita Subramanian Samuel Hong

Translational frameshift

Genetic Code

T arm

EF-Tu

10.1016/j.bpj.2019.11.1109

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Citations (0)

Structural insights into mRNA reading frame regulation by tRNA modification and slippery codon–anticodon pairing

eLife (2020)

Eric D. Hoffer Samuel Hong S. Sunita Tatsuya Maehigashi Ruben L. Gonzalez

Modifications in the tRNA anticodon loop, adjacent to the three-nucleotide anticodon, influence translation fidelity by stabilizing the tRNA to allow for accurate reading of the mRNA genetic code. One example is the N1-methylguanosine modification at guanine nucleotide 37 (m 1 G37) located in the anticodon loop andimmediately adjacent to the anticodon nucleotides 34, 35, 36. The absence of m 1 G37 in tRNA Pro causes +1 frameshifting on polynucleotide, slippery codons. Here, we report structures of the bacterial ribosome containing tRNA Pro bound to either cognate or slippery codons to determine how the m 1 G37 modification prevents mRNA frameshifting. The structures reveal that certain codon–anticodon contexts and the lack of m 1 G37 destabilize interactions of tRNA Pro with the P site of the ribosome, causing large conformational changes typically only seen during EF-G-mediated translocation of the mRNA-tRNA pairs. These studies provide molecular insights into how m 1 G37 stabilizes the interactions of tRNA Pro with the ribosome in the context of a slippery mRNA codon.

Translational frameshift

Genetic Code

T arm

Stop codon

10.7554/elife.51898

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Citations (36)

Unmodified tRNA(Pro) bound to Thermus thermophilus 70S (near cognate)

Eric D. Hoffer S. Subaramanian Seok Hoon Hong Tatsuya Maehigashi Christine Dunham

Thermus thermophilus

Cognate

10.2210/pdb6nuo/pdb