Qizouhong He

Beijing Forestry University

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Ruili Li

Institute of Agricultural Resources and Regional Planning

Jinxing Lin

Beijing Forestry University

Liang Lu

Qinhuangdao Second Hospital

Zengxing Qi

Beijing Forestry University

Wenchao Xu

Physikalisch-Technische Bundesanstalt

Jian‐Feng Mao

Chinese Academy of Sciences

Pingli Liu

Xuzhou Medical College

Xi Zhang

Huazhong Agricultural University

Huimin Xu

Hunan Children's Hospital

Yan-Ming Hong

Beijing Forestry University

Cooperative Institutions

Beijing Forestry University

Chinese Academy of Sciences

University of Chinese Academy of Sciences

Institute of Botany

State Key Laboratory of Tree Genetics and Breeding

China Agricultural University

Ministry of Agriculture and Rural Affairs

Zhejiang University

University of Bonn

Huazhong Agricultural University

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MTA, an RNA m6A Methyltransferase, Enhances Drought Tolerance by Regulating the Development of Trichomes and Roots in Poplar

International Journal of Molecular Sciences (2020)

Liang Lu Yan Zhang Qizouhong He Zengxing Qi Geng Zhang

N6-methyladenosine (m6A) is the most prevalent internal modification present in the mRNAs of all higher eukaryotes, where it is present within both coding and noncoding regions. In mammals, methylation requires the catalysis of a multicomponent m6A methyltransferase complex. Proposed biological functions for m6A modification include pre-mRNA splicing, RNA stability, cell fate regulation, and embryonic development. However, few studies have been conducted on m6A modification in trees. In particular, the regulation mechanism of RNA m6A in Populus development remains to be further elucidated. Here, we show that PtrMTA (Populus trichocarpa methyltransferase) was colocalized with PtrFIP37 in the nucleus. Importantly, the PtrMTA-overexpressing plants significantly increased the density of trichomes and exhibited a more developed root system than that of wild-type controls. Moreover, we found that PtrMTA-overexpressing plants had better tolerance to drought stress. We also found PtrMTA was a component of the m6A methyltransferase complex, which participated in the formation of m6A methylation in poplar. Taken together, these results demonstrate that PtrMTA is involved in drought resistance by affecting the development of trichomes and roots, which will provide new clues for the study of RNA m6A modification and expand our understanding of the epigenetic molecular mechanism in woody plants.

Trichome

Populus trichocarpa

N6-Methyladenosine

RNA methylation

10.3390/ijms21072462

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Citations (48)

High-resolution genome mapping and functional dissection of chlorogenic acid production in Lonicera maackii

PLANT PHYSIOLOGY (2023)

Ruili Li Jing Xu Zengxing Qi Shiwei Zhao Ran Zhao

Abstract Amur honeysuckle (Lonicera maackii) is a widely used medicinal plant of the Caprifoliaceae family that produces chlorogenic acid. Research on this plant mainly focuses on its ornamental value and medicinal compounds, but a reference genome sequence and molecular resources for accelerated breeding are currently lacking. Herein, nanopore sequencing and high-throughput chromosome conformation capture (Hi-C) allowed a chromosome-level genome assembly of L. maackii (2n = 18). A global view of the gene regulatory network involved in the biosynthesis of chlorogenic acid and the dynamics of fruit coloration in L. maackii was established through metabolite profiling and transcriptome analyses. Moreover, we identified the genes encoding hydroxycinnamoyl-CoA quinate transferase (LmHQT) and hydroxycinnamoyl-CoA shikimic/quinate transferase (LmHCT), which localized to the cytosol and nucleus. Heterologous overexpression of these genes in Nicotiana benthamiana leaves resulted in elevated chlorogenic acid contents. Importantly, HPLC analyses revealed that LmHCT and LmHQTs recombinant proteins modulate the accumulation of chlorogenic acid (CGA) using quinic acid and caffeoyl CoA as substrates, highlighting the importance of LmHQT and LmHCT in CGA biosynthesis. These results confirmed that LmHQTs and LmHCT catalyze the biosynthesis of CGA in vitro. The genomic data presented in this study will offer a valuable resource for the elucidation of CGA biosynthesis and facilitating selective molecular breeding.

Chlorogenic Acid

O-methyltransferase

Shikimic acid

10.1093/plphys/kiad295

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The Tetracentron genome provides insight into the early evolution of eudicots and the formation of vessel elements

Genome biology (2020)

Pingli Liu Xi Zhang Jian‐Feng Mao Yan-Ming Hong Rengang Zhang

Abstract Background Tetracentron sinense is an endemic and endangered deciduous tree. It belongs to the Trochodendrales, one of four early diverging lineages of eudicots known for having vesselless secondary wood. Sequencing and resequencing of the T. sinense genome will help us understand eudicot evolution, the genetic basis of tracheary element development, and the genetic diversity of this relict species. Results Here, we report a chromosome-scale assembly of the T. sinense genome. We assemble the 1.07 Gb genome sequence into 24 chromosomes and annotate 32,690 protein-coding genes. Phylogenomic analyses verify that the Trochodendrales and core eudicots are sister lineages and showed that two whole-genome duplications occurred in the Trochodendrales approximately 82 and 59 million years ago. Synteny analyses suggest that the γ event, resulting in paleohexaploidy, may have only happened in core eudicots. Interestingly, we find that vessel elements are present in T. sinense , which has two orthologs of AtVND7 , the master regulator of vessel formation. T. sinense also has several key genes regulated by or regulating TsVND7.2 and their regulatory relationship resembles that in Arabidopsis thaliana. Resequencing and population genomics reveals high levels of genetic diversity of T. sinense and identifies four refugia in China. Conclusions The T. sinense genome provides a unique reference for inferring the early evolution of eudicots and the mechanisms underlying vessel element formation. Population genomics analysis of T. sinense reveals its genetic diversity and geographic structure with implications for conservation.

Eudicots

Synteny

Comparative Genomics

10.1186/s13059-020-02198-7

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Citations (30)

Dynamics and Endocytosis of Flot1 in Arabidopsis Require CPI1 Function

International Journal of Molecular Sciences (2020)

Yangyang Cao Qizouhong He Zengxing Qi Yan Zhang Liang Lu

Membrane microdomains are nano-scale domains (10-200 nm) enriched in sterols and sphingolipids. They have many important biological functions, including vesicle transport, endocytosis, and pathogen invasion. A previous study reported that the membrane microdomain-associated protein Flotillin1 (Flot1) was involved in plant development in Arabidopsis thaliana; however, whether sterols affect the plant immunity conveyed by Flot1 is unknown. Here, we showed that the root length in sterol-deficient cyclopropylsterolisomerase 1 (cpi1-1) mutants expressing Flot1 was significantly shorter than in control seedlings. The cotyledon epidermal cells in cpi1-1 mutants expressing Flot1 were smaller than in controls. Moreover, variable-angle total internal reflection fluorescence microscopy (VA-TIRFM) and single-particle tracking (SPT) analysis demonstrated that the long-distance Flot1-GFP movement was decreased significantly in cpi1-1 mutants compared with the control seedlings. Meanwhile, the value of the diffusion coefficient Ĝ was dramatically decreased in cpi1-1 mutants after flagelin22 (flg22) treatment compared with the control seedlings, indicating that sterols affect the lateral mobility of Flot1-GFP within the plasma membrane. Importantly, using confocal microscopy, we determined that the endocytosis of Flot1-GFP was decreased in cpi1-1 mutants, which was confirmed by fluorescence cross spectroscopy (FCS) analysis. Hence, these results demonstrate that sterol composition plays a critical role in the plant defense responses of Flot1.

Lipid microdomain

10.3390/ijms21051552

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Phase separation of the poplar microtubule-associated protein PagPCaP1a aids microtubule depolymerization in response to high salt

Science Advances (2025)

Na Lian Xinyuan Zhang Xinwei Wang Yu Zhang Xinyuan Wu

Woody plants must acclimate to environmental stresses, including soil salinity, for proper growth and development. Microtubule reorganization supports plant survival in saline-rich soils, but the underlying molecular mechanism in tree species remains unclear. In this study, we identified a salinity stress response mechanism in hybrid poplar seedlings. This mechanism involves regulation of microtubule dynamics by the microtubule-associated protein PLASMA MEMBRANE–ASSOCIATED CATION BINDING PROTEIN 1a (PagPCaP1a). Salinity stress induced PagPCaP1a expression and phase separation of PagPCaP1a protein to form PagPCaP1a condensates in a calcium-dependent manner. The formation of PagPCaP1a condensates was partially driven by the VEEEKK motif within the carboxyl terminus of the protein, which rapidly depolymerizes microtubules under salinity stress. Our study reveals that the liquid-liquid phase separation of PagPCaP1a represents an additional regulatory layer for microtubule depolymerization, and we propose an effective strategy to manipulate the phase separation of PagPCaP1a to improve plant stress tolerance.

Depolymerization

Microtubule polymerization

Microtubule nucleation

Microtubule-associated protein

10.1126/sciadv.ads3653

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A rapid and effective optical-clearing technique for deep tissue fluorescence imaging in trees

Trees (2020)

Liang Lu Yangyang Cao Qizouhong He Wenchao Xu Yan Zhang

Clearing

Optical sectioning

Fluorescence-lifetime imaging microscopy

10.1007/s00468-020-01957-0

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Citations (2)