OBJECTIVES--The aim was, firstly, to study the validity of the sentinel network data by analysing the correlation between the weekly influenza cases detected by the network and the number of cases notified to the compulsory disease notification system and, secondly, to describe the epidemiology of the period 1991-92 in terms of the sentinel network data. DESIGN--The study design was descriptive. SUBJECTS--The population resident in the city of Madrid. MAIN RESULTS--There was a high correlation between the cases notified to the compulsory disease notification system and those listed by the sentinel network (r = 0.91, p < 0.001, r2 = 0.82). The epidemic activity during the period 1991-92 was centred approximately on week 48 in 1991 and week 6 in 1992 (24 November 1991-8 February 1992). Altogether 913 cases of influenza were notified through the sentinel network. The greatest number of cases occurred in people aged between 20 and 39 years. CONCLUSION--For the epidemiological surveillance of influenza it is necessary to develop systems that can quickly detect epidemic periods and provide information about populations at risk, in addition to systems that isolate and identify seasonal epidemic viruses. Both types of data will help the development of adequate public health policies. Sentinel networks provide these data and offer additional advantages such as lower cost.

10.1136/jech.49.suppl_1.14

Cite

Citations (14)

Application of thiophilic chromatography to deplete serum immunoglobulins in sample preparation for bidimensional electrophoresis

Analytica Chimica Acta (2009)

Francisco J. Salgado Sara E. Vazquez Alba Iglesias Amparo Pérez‐Díaz Antonio Mera

Human serum albumin

Proteome

Bovine serum albumin

Sample Preparation

Serum Albumin

10.1016/j.aca.2009.10.065

Cite

Citations (8)

Macrophage Caldesmon Is an Actin Bundling Protein

Biochemistry (2001)

Pilar Arias Michèle Pacaud

A rapid purification procedure was developed for the isolation of caldesmon (CaD) from rabbit alveolar macrophage. The purified protein migrated with an apparent M(r) of 74,000 +/- 4000 on SDS-PAGE and cross-reacted with anti-gizzard CaD antibodies. A higher M(r) isoform was isolated from chicken gizzard. Their actin-binding parameters and effects on actomyosin-ATPase activity were investigated under identical experimental conditions. Electron microscope studies revealed that macrophage CaD was able to cross-link actin filaments into both networks and bundles. Compact F-actin bundles were predominantly or exclusively seen at cross-linker to actin molar ratios in the 1:20 to 1:10 range. Apparent K(a) at extrapolated saturation of the CaD-binding sites on F-actin was 1.2 x 10(6) M(-1) for macrophage CaD and 1.6 x 10(6) M(-1) for chicken gizzard CaD. CaD from either source was able to stimulate the actin-activated ATPase activity of macrophage myosin. Unexpectedly, chicken gizzard CaD also increased the ATPase activity of gizzard myosin. The degree of stimulation was approximately doubled in the presence of a large excess of Ca(2+)-calmodulin but was unaffected by the presence of macrophage tropomyosin. However, macrophage CaD did not behave as a Ca(2+)- and calmodulin-regulated actin-binding protein. These results, together with published data on other well-characterized actin bundling proteins, suggest that nonmuscle CaD could be essentially involved in the formation and organization of actin bundles at adhesion sites and cell surface projections. However, they afforded no evidence that the macrophage isoform might play a specific role in the Ca(2+)-dependent regulation of actin and myosin II interactions.

Caldesmon

Tropomyosin

Gizzard

Actin-binding protein

10.1021/bi010098+

Cite

Citations (7)

Experience at the Department of Pediatrics of a private facility in the Metropolitan Area of Buenos Aires during the COVID-19 pandemic

Archivos Argentinos de Pediatria (2021)

Daniel J. Capra Gabriela Ucha Enrique Antoni Augusto Malfetano Nelson Wolfsteiner

Introduction.Towards the end of 2019, a novel coronavirus that causes COVID-19 was identified and became a pandemic.In Argentina, approximately 37 % of the total population lives in the Metropolitan Area of Buenos Aires (AMBA), where most cases have been diagnosed.The objective of this study was to describe the clinical and epidemiological characteristics of COVID-19 patients and the impact on the operations of the Department of Pediatrics of a private facility located in the AMBA.Methods.Retrospective, observational study conducted at a facility in the west of AMBA between March 12 th and August 31 st , 2020.All patients younger than 16 years diagnosed with COVID-19 were included.Demographic, epidemiological, and clinical characteristics; indication for hospitalization/outpatient followup; number of outpatient visits; hospitalization due to seasonal viral infections; staff on leave; changes in bed availability and health care providers' activities were recorded.Results.There were 5454 pediatric outpatient visits, COVID-19 was suspected in 753/5454 (13.8 %) and 152/753 (20.2 %) were confirmed cases.Their median age was 82 months (interquartile range: 20.5-147 months); 50 % were males.Fever was the most common symptom.In total, 22/152 (14.5 %) patients were hospitalized.Outpatients visits decreased by 87 %; there were no hospitalizations due to seasonal viral infections; and 52.9 % (91/172) of staff took a leave.Conclusions.Most cases were mild, and fever was the main symptom.The department operations were considerably affected in terms of human resources.It is worth noting the need for a logistic organization at the Department of Pediatrics to face such contingency.

Pandemic

2019-20 coronavirus outbreak

10.5546/aap.2021.eng.310

Cite

Citations (3)

Malic dehydrogenase isoenzymatic pattern in lung-nematode parasite species

Parasitology Research (1996)

Cristina Cutillas Pilar Arias Marta Špakulová

Malate dehydrogenase

Malic acid

10.1007/s004360050076

Cite

Citations (5)