Dipali Singh

Rajendra Institute of Medical Sciences

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Mark G. Poolman

Oxford Brookes University

Valeria Villanova

University of Palermo

Giovanni Finazzi

Institut de Recherches en Technologies et Sciences pour le Vivant

Adeline Le Monnier

Oliver Ebenhöh

Heinrich Heine University Düsseldorf

Angela Falciatore

Centre National de la Recherche Scientifique

John Wain

University of East Anglia

Noemı́ Tejera

Norwich Research Park

Julien Pagliardini

Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés

ÉM

Éric Maréchal

Centre National de la Recherche Scientifique

Cooperative Institutions

Centre National de la Recherche Scientifique

Forschungszentrum Jülich

Quadram Institute

Norwich Research Park

Université Grenoble Alpes

Laboratoire Physiologie Cellulaire & Végétale

CEA Grenoble

Commissariat à l'Énergie Atomique et aux Énergies Alternatives

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

Heinrich Heine University Düsseldorf

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

New Techniques and Programs to Control Tuberculosis

Dipali Singh

Tuberculosis is preety big global health problem. Tuberculosis is the second most infectious disease not only in india, rather in whole world. Agent factor is ‘microbactirium tuberculosis’-Acid fast bacilli.It’s mainly spreads in endemic areas. Global T.B programme have target to end T.B till 2035.Many other programs also running in different countries like in india ‘National tuberculosis elimination program’ (new name of RNTCP-Revised national tuberculosis control program) have target to control T.B till 2025 .These programs have strategies like-Active case ditection,house to house surveys, softwares where all T.B patients are registered it design for management and compliance survices, 99 DOTs, in 2018-2019 Bedaquilline and Delamanid are added for treatment (except some compilations related with heart,eye, kidney,liver, pancreas), chemoprophylaxis of TB - DOC is Isoniazid, air born infection control kits also available, in some countries incentive system available like who will give the information of TB patient by any resources that person is awarded by some money by government. So, the aim of these programs to eliminate TB patient early for better treatment and least spread because TB detection and treatment is time taken and if the diagnosis test is lack or elimination is less it will spread more and more in community.

Chemoprophylaxis

Tuberculosis control

Source

Cite

Citations (0)

A novel algorithm to calculate elementary modes: Analysis of Campylobacter jejuni metabolism

Biosystems (2023)

Yanica Said Dipali Singh Cristiana Sebu Mark G. Poolman

We describe a novel algorithm, 'LPEM', that given a steady-state flux vector from a (possibly genome-scale) metabolic model, decomposes that vector into a set of weighted elementary modes such that the sum of these elementary modes is equal to the original flux vector. We apply the algorithm to a genome scale metabolic model of the human pathogen Campylobacter jejuni. This organism is unusual in that it has an absolute growth requirement for oxygen, despite being able to operate the electron transport chain anaerobically. We conclude that (1) Microaerophilly in C. jejuni can be explained by the dependence of pyridoxine 5

10.1016/j.biosystems.2023.105047

Cite

Citations (0)

Boosting Biomass Quantity and Quality by Improved Mixotrophic Culture of the Diatom Phaeodactylum tricornutum

Frontiers in Plant Science (2021)

Valeria Villanova Dipali Singh Julien Pagliardini David A. Fell Adeline Le Monnier

Diatoms are photoautotrophic unicellular algae and are among the most abundant, adaptable, and diverse marine phytoplankton. They are extremely interesting not only for their ecological role but also as potential feedstocks for sustainable biofuels and high-value commodities such as omega fatty acids, because of their capacity to accumulate lipids. However, the cultivation of microalgae on an industrial scale requires higher cell densities and lipid accumulation than those found in nature to make the process economically viable. One of the known ways to induce lipid accumulation in Phaeodactylum tricornutum is nitrogen deprivation, which comes at the expense of growth inhibition and lower cell density. Thus, alternative ways need to be explored to enhance the lipid production as well as biomass density to make them sustainable at industrial scale. In this study, we have used experimental and metabolic modeling approaches to optimize the media composition, in terms of elemental composition, organic and inorganic carbon sources, and light intensity, that boost both biomass quality and quantity of P. tricornutum . Eventually, the optimized conditions were scaled-up to 2 L photobioreactors, where a better system control (temperature, pH, light, aeration/mixing) allowed a further improvement of the biomass capacity of P. tricornutum to 12 g/L.

Phaeodactylum tricornutum

Photobioreactor

Mixotroph

Autotroph

10.3389/fpls.2021.642199

Cite

Citations (22)

Supplementary material from "Investigating mixotrophic metabolism in the model diatom Phaeodactylum tricornutum "

Valeria Villanova Antonio Emidio Fortunato Dipali Singh Davide Dal Bo Melissa Conte

Phaeodactylum tricornutum

Mixotroph

10.6084/m9.figshare.c.3799462.v2

Cite

Citations (0)

Substrate Utilisation and Energy Metabolism in Non-Growing Campylobacter jejuni M1cam

Microorganisms (2022)

Emily Stoakes George M. Savva Ruby Coates Noemı́ Tejera Mark G. Poolman

, the major cause of bacterial foodborne illness, is also a fastidious organism that requires strict growth requirements in the laboratory. Our aim was to study substrate utilisation and energy metabolism in non-growing

Acetate kinase

Fastidious organism

Metabolic pathway

10.3390/microorganisms10071355

Cite

Citations (6)

Response of Nitrogen Fertilization on Gladiolus

Journal of Ornamental Horticulture (2001)

Shalini Sharma Dipali Singh

Gladiolus

Source

Cite

Citations (9)

From bag-of-genes to bag-of-genomes: metabolic modelling of communities in the era of metagenome-assembled genomes

Computational and Structural Biotechnology Journal (2020)

Clémence Frioux Dipali Singh Tamás Korcsmáros Falk Hildebrand

Metagenomic sequencing of complete microbial communities has greatly enhanced our understanding of the taxonomic composition of microbiotas. This has led to breakthrough developments in bioinformatic disciplines such as assembly, gene clustering, metagenomic binning of species genomes and the discovery of an incredible, so far undiscovered, taxonomic diversity. However, functional annotations and estimating metabolic processes from single species – or communities – is still challenging. Earlier approaches relied mostly on inferring the presence of key enzymes for metabolic pathways in the whole metagenome, ignoring the genomic context of such enzymes, resulting in the 'bag-of-genes' approach to estimate functional capacities of microbiotas. Here, we review recent developments in metagenomic bioinformatics, with a special focus on emerging technologies to simulate and estimate metabolic information, that can be derived from metagenomic assembled genomes. Genome-scale metabolic models can be used to model the emergent properties of microbial consortia and whole communities, and the progress in this area is reviewed. While this subfield of metagenomics is still in its infancy, it is becoming evident that there is a dire need for further bioinformatic tools to address the complex combinatorial problems in modelling the metabolism of large communities as a 'bag-of-genomes'.

10.1016/j.csbj.2020.06.028

Cite

Citations (78)

An Overview of Computational Approaches in Structure Based Drug Design

Nepal Journal of Biotechnology (1970)

Dipali Singh Anushree Tripathi Gautam Kumar

Drug design is a costly and difficult process. Drug must fulfill several criteria of being active, nontoxic and bioavailable. The conventional way of synthesizing drugs is a monotonous process. But computer aided drug design is a proficient way to overcome the tedious process of conventional method. Drugs can be designed computationally by structure or target based drug designing (SBDD). This review summarizes the methods of structure based drug design, usage of related softwares and a case study that explores to find a suitable drug (lead) molecule for the mutated state of H-Ras protein in order to prevent complex formation with Raf protein.Keywords: computer aided drug design; structure based drug design; Ras-proteinDOI: http://dx.doi.org/10.3126/njb.v2i1.5680Nepal Journal of Biotechnology Jan.2012, Vol.2(1): 53-61

10.3126/njb.v2i1.5680

Cite

Citations (5)

Food threats in organic produce through biological contaminants

International Journal of Plant Pathology and Microbiology (2022)

Kriti Thakur Dipali Singh Shilpa Kaushal

Food borne pathogens acts a main risk factor in terms of public health in the developed and developing countries caused by their adequateness all around the world. The most important foodborne pathogens are Escherichia coli and other coliform bacteria. Some other most important cause of contamination for these microorganisms are described as: places with unfavourable hygiene, contaminated waste water, meat, cereal, fruits and vegetable products. Overall coliform bacteria and E.coli count is known as the sign of unfavourable sterile conditions and fecal contamination in foods. Foodborne diseases are still regarded as a major global issue. A common approach by all the countries and other international organizations is a precondition for identifying and controlling foodborne problems that cause a threat to human health and international market. By observing their problematic biology, epidemiology and analyses it is observed that most of the foodborne diseases are curable. It is important for public health that purchasers and food producers should act in accordance with the concept of internationally accepted safety methods.

Food contaminant

10.22271/27893065.2022.v2.i1a.12

Cite

Citations (0)

MdaB and NfrA, Two Novel Reductases Important in the Survival and Persistence of the Major Enteropathogen Campylobacter jejuni

Journal of Bacteriology (2021)

Fauzy Nasher Aidan J. Taylor Abdi Elmi Burhan Lehri Umer Zeeshan Ijaz

Changes in cellular redox potential result in alteration in the oxidation state of intracellular metabolites and enzymes; consequently, cells make adjustments that favor growth and survival. The work we present here answers some of the many questions that have remained elusive over the years of investigation into the enigmatic microaerophile bacterium Campylobacter jejuni .

Persistence (discontinuity)

10.1128/jb.00421-21

Cite

Citations (5)