A number of structurally different allergens trigger the release of mediators from basophils by cross-linking of IgE receptors. In this study, we analyzed the effects of cyclosporine A (CSA) and FK-506 on allergen-induced histamine release in human blood basophils obtained from birch- or grass-pollen-allergic donors (n = 12). Preincubation of basophils with CSA (0.003–3 μg/ml) or FK-506 (0.003–3 μg/ml) led to inhibition of histamine release induced by purified recombinant tree pollen allergens (r Bet v 1, r Bet v 2) and timothy grass pollen allergens (r Ph1 p 1, r Ph1 p 2, r Ph1 p 5). The effects of CSA and FK-506 were dose dependent, with IC50 values ranging between 0.03 and 0.3 μg/ml for both CSA and FK-506. Cyclosporine H, an inactive CSA analog, did not show any effect on allergen-induced histamine secretion. IgE dependency of the reaction was demonstrated in passive transfer experiments using highly enriched human basophils (> 95% pure) and specific IgE from a patient allergic to Bet v 2. In summary, our data show that CSA and FK-506 inhibit recombinant-allergen-induced histamine release from peripheral blood basophils in allergic donors.
Journal Article Recombinant Murine Granulocyte-Macrophage Colony-Stimulating Factor Augments Neutrophil Recovery and Enhances Resistance to Infections in Myelosuppressed Mice Get access P. Mayer, P. Mayer Sandoz Research Center, Vienna, Austria Reprints or correspondence: Dr. Peter Mayer, Sandoz Research Center, Brunner StraBe 59, A-1235 Vienna, Austria. Search for other works by this author on: Oxford Academic PubMed Google Scholar E. Sehutze, E. Sehutze Sandoz Research Center, Vienna, Austria Search for other works by this author on: Oxford Academic PubMed Google Scholar C. Lam, C. Lam Sandoz Research Center, Vienna, Austria Search for other works by this author on: Oxford Academic PubMed Google Scholar F. Kricek, F. Kricek Sandoz Research Center, Vienna, Austria Search for other works by this author on: Oxford Academic PubMed Google Scholar E. Liehl E. Liehl Sandoz Research Center, Vienna, Austria Search for other works by this author on: Oxford Academic PubMed Google Scholar The Journal of Infectious Diseases, Volume 163, Issue 3, March 1991, Pages 584–590, https://doi.org/10.1093/infdis/163.3.584 Published: 01 March 1991 Article history Received: 26 July 1990 Revision received: 05 October 1990 Published: 01 March 1991
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The novel stromal cell factor, IL-11, has been reported to have diverse effects on the lymphopoietic and myeloid/erythroid cells in vitro. These include expansion of T cell-dependent Ig-secreting B cells, proliferation and differentiation of megakaryocytic progenitors and of a variety of myeloid and erythroid precursor cells, and multiplication of pluripotential hematopoietic progenitors. In addition, IL-11 inhibits adipogenesis in vitro. In vivo administration of IL-11 elevated the number of circulating neutrophils and platelets and increased the number of megakaryocytes in the spleens of normal mice.
