Xianghan Chen

Sichuan University

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Ming Wang

Anhui Agricultural University

Lanqun Mao

Beijing Normal University

Heng Xu

University of Jinan

Weiqi Cai

Qingdao University of Science and Technology

Ji Liu

Beijing National Laboratory for Molecular Sciences

Ke Lei

Qingdao University

He Ren

Qingdao University

Huakang Liu

Sichuan University

Jinhan Sheng

Chinese Academy of Sciences

Chaoqun Yan

Sichuan University

Cooperative Institutions

Chinese Academy of Sciences

Sichuan University

Affiliated Hospital of Qingdao University

Beijing National Laboratory for Molecular Sciences

Qingdao University

University of Chinese Academy of Sciences

Central South University

Zhejiang University

Capital Medical University

Chinese Academy of Medical Sciences & Peking Union Medical College

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Integration of Palladium Nanoparticles with Surface Engineered Metal–Organic Frameworks for Cell-Selective Bioorthogonal Catalysis and Protein Activity Regulation

ACS Applied Materials & Interfaces (2022)

Xianghan Chen Weiqi Cai Ji Liu Lanqun Mao Ming Wang

Bioorthogonal catalysis provides a powerful tool to perform non-natural chemical reactions in living systems to dissect complex intracellular processes. Its potency to precisely regulate cellular function, however, is limited by the lack of bioorthogonal catalysts with cell selectivity. Herein, we report that palladium nanoparticles deposited on metal-organic frameworks, Pd@UiO-66, are highly efficient for intracellular bioorthogonal catalysis. In addition, introducing a cancer cell-targeting aptamer, AS1411, onto Pd@UiO-66 enables a threefold enhancement of catalysis efficiency in cancer cells. Moreover, AS1411@Pd@UiO-66 is effective in activating chemically caged 4-hydroxytamoxifen to regulate the activity of a protein destabilizing domain, ER50, and therefore protein function selectively in cancer cells. We show that the control over the activity of a bacterial effector, OspF, using AS1411@Pd@UiO-66 inactivates mitogen-activated protein kinase (MAPK) signaling of cancer cells to selectively prohibit tumor cell growth. We believe that the strategy developed herein for cell-selective bioorthogonal catalysis can expand the chemical biology toolbox for spatiotemporal control of protein function for advanced therapeutic applications.

Bioorthogonal Chemistry

Aptamer

10.1021/acsami.1c23213

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Citations (17)

Recovery of phosphate and removal of Cr(VI) from water by calcium-modified panda manure biochar: Synergistic effect of adsorption and reduction

Journal of the Taiwan Institute of Chemical Engineers (2025)

Chaoqun Yan Dinghua Peng Xianghan Chen Yumei Zhang Huakang Liu

Phosphate fertilizer

10.1016/j.jtice.2025.106015

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Citations (0)

Tetraphenylethylene‐Featured Fluorescent Supramolecular Nanoparticles for Intracellular Trafficking of Protein Delivery and Neuroprotection

Angewandte Chemie International Edition (2021)

Ji Liu Jinhan Sheng Leihou Shao Qizhen Zheng Wenting Li

Abstract The delivery of protein into mammalian cells enables the dissection and manipulation of biological processes; however, this potency is challenged by the lack of an efficient protein delivery tool and a means to monitor its intracellular trafficking. Herein, we report that the hierarchical self‐assembly of tetraphenylethylene (TPE)‐featured metal–organic cages (MOCs) and β‐cyclodextrin‐conjugated polyethylenimine can generate fluorescent supramolecular nanoparticles (FSNPs) to deliver protein into neural cells, a cell line that is hard to transfect using conventional strategy. Further, the aggregation‐induced emission (AIE) of TPE enabled the fluorescent monitoring of cytosolic protein release. It is found that FSNPs can deliver and release protein into cytosol for subcellular targeting as fast as 18 h post‐delivery. Moreover, the delivery of molecular chaperone DJ‐1 using FSNPs activates MAPK/ERK signaling of neural cells to protect cells from oxidative stress.

Tetraphenylethylene

Polyethylenimine

10.1002/anie.202111213

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Citations (25)

Enzyme-Instructed Activation of Pro-protein Therapeutics In Vivo

Journal of the American Chemical Society (2019)

Jin Chang Weiqi Cai Chunjing Liang Qiao Tang Xianghan Chen

The selective and temporal control of protein activity in living cells provides a powerful tool to manipulate cellular function and to develop pro-protein therapeutics (PPT) for targeted therapy. In this work, we reported a facile but general chemical approach to design PPT by modulating protein activity in response to endogenous enzyme of disease cells, and its potential for targeted cancer therapy. We demonstrated that the chemical modification of a protein with quinone propionic acid (QPN), a ligand that could be reduced by tumor-cell-specific NAD(P)H dehydrogenase [quinone] 1 (NQO1), was reversible in the presence of NQO1. Importantly, the QPN-modified cytochrome c (Cyt c-QPN) and ribonuclease A (RNase A-QPN) showed NQO1-regulated protein activity in a highly selective manner. Furthermore, the intracellular delivery of RNase A-QPN using a novel type of lipid-based nanoparticles, and subsequent protein activation by cellular NQO1, selectively inhibit cancer cell growth in vitro and effectively suppress tumor growth in vivo. We believe that our approach increases the number of potentially useful chemical tools for reversibly controlling the structure and function of protein using a disease-cell-specific enzyme, opening opportunities in the study of dynamic biological processes and developing precise protein therapeutics.

10.1021/jacs.9b08669

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Citations (60)

Expression landscape of cancer-FOXP3 and its prognostic value in pancreatic adenocarcinoma

Cancer Letters (2024)

Ruining Gong Jia Wang Yihai Xing Jigang Wang Xianghan Chen

10.1016/j.canlet.2024.216838

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Citations (1)

Dynamic and adjustable particle swarm optimization

Chen-Yi Liao Wei‐Ping Lee Xianghan Chen Michael F. Chiang

Particle Swarm Optimization (PSO) is a stochastic, population-based evolutionary search technique. It has difficulties in controlling the balance between exploration and exploitation. In order to improve the performance of PSO and maintain the diversities of particles, we propose a novel algorithm called Dynamic and Adjustable Particle Swarm Optimization (DAPSO). The distance from each particle to the global best position is calculated in order to adjust the velocity suitably of each particle. Four benchmark functions such as Sphere, Rosenbrock, Rastrigrin, Griewank are used for the comparison of DAPSO with the Standard PSO. The experiments prove that DAPSO has better performance than the Standard PSO.

Benchmark (surveying)

Position (finance)

Imperialist competitive algorithm

Particle (ecology)

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Citations (8)

An activity-based two-photon fluorescent probe for real-time and reversible imaging of oxidative stress in the rat brain

Chemical Communications (2020)

Chunjing Liang Xianghan Chen Qiao Tang Wenliang Ji Ying Jiang

We report herein a modular approach to design the Michael addition between glutathione and coumarin derivatives for fluorescence imaging of the reversible and dynamic change of oxidative stress in living cells and rat brain.

Fluorescence-lifetime imaging microscopy

Two-photon excitation microscopy

10.1039/d0cc01747g

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Citations (18)

Effect of phosphate-mineralized bacteria on multi-metals migration behavior in vanadium tailing slags: Coexistence of immobilization and mobilization

Journal of Hazardous Materials (2024)

Dinghua Peng Yumei Zhang Xianghan Chen Ying Zhang Huayan Huang

Mobilization

10.1016/j.jhazmat.2024.135880

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Citations (3)

Orthogonal Chemical Activation of Enzyme-Inducible CRISPR/Cas9 for Cell-Selective Genome Editing

Journal of the American Chemical Society (2022)

Weiqi Cai Ji Liu Xianghan Chen Lanqun Mao Ming Wang

The precision and therapeutic potential of CRISPR/Cas9 genome editing are greatly challenged by the less control over Cas9-mediated DNA cleavage. Herein, we introduce a conditional and cell-selective genome editing system controlled by disease-associated enzymes, termed enzyme-inducible CRISPR (eiCRISPR). eiCRISPR comprises Cas9 protein, a self-blocked inactive single-guide RNA (bsgRNA), and a chemically caged deoxyribozyme (DNAzyme) that activates bsgRNA and eiCRISPR in a controllable manner. We design chemical modifications of DNAzyme to suppress its ability to cleave the blocking region of bsgRNA, while the decaging of DNAzyme triggered by the tumor cell-overexpressed enzyme, for instance, NAD(P)H:quinone oxidoreductase (NQO1), restores the activity of bsgRNA and switches on eiCRISPR selectively for genome editing in cancer cells. Moreover, using a biodegradable lipid nanoparticle to deliver eiCRISPR in a tumor-bearing xenograft, we show that the in vivo activation of eiCRISPR enables the editing of human papillomavirus 18 E6 for potential cancer therapy. The strategy of postsynthetic and site-specific modification of DNAzyme is compatible with endogenous chemistries for regulating eiCRISPR for cell-selective genome editing and targeted gene therapy.

Deoxyribozyme

10.1021/jacs.2c10545

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Citations (41)

Nitrogen-doped carbon nanotubes as an excellent substrate for electroless deposition of Pd nanoparticles with a high efficiency toward the hydrogen evolution reaction

Electrochemistry Communications (2018)

Siliao Zhou Xianghan Chen Ping Yu Feng Gao Lanqun Mao

Overpotential

10.1016/j.elecom.2018.04.015

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Citations (22)