Massimo Guardigli

University of Bologna

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Aldo Roda

University of Bologna

140

Mara Mirasoli

University of Bologna

Elisa Michelini

University of Bologna

Martina Zangheri

University of Bologna

Donato Calabria

University of Bologna

Patrizia Simoni

University of Bologna

N. Sabbatini

University of Bologna

Monica Musiani

University of Bologna

Raymond Ziessel

Newcastle University

Luisa Stella Dolci

University of Bologna

Cooperative Institutions

University of Bologna

160

Policlinico S.Orsola-Malpighi

Centre National de la Recherche Scientifique

Sapienza University of Rome

Istituti di Ricovero e Cura a Carattere Scientifico

University of Parma

University of Ferrara

Istituto Nazionale Biostrutture e Biosistemi

Institut de Chimie

University of Pisa

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ULTRASENSITIVE CHEMILUMINESCENT IMMUNOCHEMICAL LOCALISATION OF PROTEIN COMPONENTS IN PAINTING CROSS-SECTIONS

Luisa Stella Dolci Giorgia Sciutto Manuela Rizzoli Massimo Guardigli Rocco Mazzeo

10.1142/9789812839589_0079

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Lanthanide complexes of encapsulating ligands: Luminescent devices at the molecular level

Pure and Applied Chemistry (1995)

N. Sabbatini Massimo Guardigli Ilse Manet R. Ungaro Alessandro Casnati

Abstract

10.1351/pac199567010135

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Citations (124)

Paper-Based Immunosensors with Bio-Chemiluminescence Detection

Sensors (2021)

Maria Maddalena Calabretta Martina Zangheri Donato Calabria Antonia Lopreside Laura Montali

Since the introduction of paper-based analytical devices as potential diagnostic platforms a few decades ago, huge efforts have been made in this field to develop systems suitable for meeting the requirements for the point-of-care (POC) approach. Considerable progress has been achieved in the adaptation of existing analysis methods to a paper-based format, especially considering the chemiluminescent (CL)-immunoassays-based techniques. The implementation of biospecific assays with CL detection and paper-based technology represents an ideal solution for the development of portable analytical devices for on-site applications, since the peculiarities of these features create a unique combination for fitting the POC purposes. Despite this, the scientific production is not paralleled by the diffusion of such devices into everyday life. This review aims to highlight the open issues that are responsible for this discrepancy and to find the aspects that require a focused and targeted research to make these methods really applicable in routine analysis.

10.3390/s21134309

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Citations (35)

BESIDES (BiomolEcular SIgnature DEtection System): A Lab-On-Chip-Based Analytical Platform for Life Biomarkers Detection in Astrobiology Investigations

Mara Mirasoli Laura Anfossi A. Nascetti Liyana Popova Michele Balsamo

Signature (topology)

Lab-on-a-Chip

10.52202/078357-0065

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Chemiluminescence-based biosensor for monitoring astronauts’ health status during space missions: Results from the International Space Station

Biosensors and Bioelectronics (2018)

Martina Zangheri Mara Mirasoli Massimo Guardigli Fabio Di Nardo Laura Anfossi

International Space Station

10.1016/j.bios.2018.09.059

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Citations (50)

Energy Transfer in Rigid Ru(II)/Os(II) Dinuclear Complexes with Biscyclometalating Bridging Ligands Containing a Variable Number of Phenylene Units

Inorganic Chemistry (1996)

Francesco Barigelletti Lucia Flamigni Massimo Guardigli Alberto Juris Marc Beley

We have prepared rodlike cyclometalated Ru(II)/Os(II) dinuclear complexes, (ttp)Ru(dpb-(ph)(n)()-dpb)Os(ttp)(2+), where the biscyclometalating bridging ligands contain dipyridylbenzene fragments, dbp, separated by a variable number, n, of phenylene spacers, and the terminal ligand is a terpyridine derivative [dpbH is di-2-pyridyl-1,3-benzene, ttp is 4'-p-tolyl-2,2':6',2"-terpyridine, and n = 0-2]. The rigid bridging ligands keep the metal centers at a distance r(MM) = 11, 15.5, and 20 Å, depending on n. Photoinduced energy transfer has been investigated by luminescence spectroscopy in nitrile solvents at room temperature and at 77 K (i.e., in frozen medium). According to a classical description of the process, the energy transfer occurs in a nearly activationless regime, is governed by electronic factors, and can be described in terms of the Dexter-type mechanism. The obtained energy transfer rates roughly span 3 orders of magnitude and indicate (i) that the temperature (i.e., the state of the solvent) has a small influence on the process and (ii) that the interposed phenylene spacers are weak attenuators of intercenter electronic coupling, H [H = H(0) exp(-betar(MM)), with beta approximately 0.33 Å(-)(1)].

Terpyridine

Bridging ligand

Biphenylene

10.1021/ic9503085

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Citations (153)

Chemiluminescence Quantitative Immunohistochemical Determination of MRP2 in Liver Biopsies

Journal of Histochemistry & Cytochemistry (2005)

Massimo Guardigli Marianna Marangi Silvia Casanova Walther F. Grigioni E. Roda

Evaluation of protein expression in tissues and cells by electrophoretic and blotting techniques or by the quantification of the mRNA coding for the target protein is a common procedure in biochemistry research and clinical diagnoses. In this article, an alternative approach, based on an immunohistochemical procedure with chemiluminescent imaging detection, is described. The assay exploited the peculiar characteristics of the chemiluminescent detection of enzyme labels (high sensitivity and specificity, low background, easy quantification of the signal) for performing the direct, simple, and rapid quantitative evaluation of protein expression in tissues. When applied to the study of the levels of MRP2, a member of the human multidrug resistance-associated protein family, in samples obtained from formalin-fixed, paraffin-embedded liver biopsies, it allowed the reliable evaluation of the protein content of the tissue. Moreover, the analysis of clinical samples from patients with primary biliary cirrhosis under therapy with ursodeoxycholic acid gave results in line with those, previously reported in the literature, obtained with conventional protein expression analysis techniques.

Ursodeoxycholic acid

Primary Biliary Cirrhosis

Quantitative Analysis

10.1369/jhc.5a6621.2005

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Citations (17)

Multienzyme chemiluminescent foldable biosensor for on-site detection of acetylcholinesterase inhibitors

Biosensors and Bioelectronics (2020)

Laura Montali Maria Maddalena Calabretta Antonia Lopreside Marcello D’Elia Massimo Guardigli

Choline oxidase

Luminol

Horseradish peroxidase