Neha Kumari

Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya

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Palvi Thakur

Thomas College

Sanjay Chadha

Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya

Sachchida Nand Mishra

Sam Higginbottom Institute of Agriculture

Anjli Dhiman

Institute of Himalayan Bioresource Technology

Namo Narayan Mishra

Sam Higginbottom Institute of Agriculture

KK Yadav

Maharana Pratap University of Agriculture and Technology

Shashikant Sharma

Lovely Professional University

Dharam Pal

Indian Agricultural Research Institute

Manju Modgil

Dr. Yashwant Singh Parmar University of Horticulture and Forestry

Pratibha Sharma

Devi Ahilya Vishwavidyalaya

Cooperative Institutions

Sam Higginbottom Institute of Agriculture

Maharana Pratap University of Agriculture and Technology

Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya

Swami Keshwanand Rajasthan Agricultural University

Institute of Himalayan Bioresource Technology

Bridge University

Dr. Yashwant Singh Parmar University of Horticulture and Forestry

Agricultural Institute

Lovely Professional University

People’s University

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Development of high frequency in vitro shoot regeneration system from leaves of apple cultivar ‘Oregon Spur’ and optimization of antibiotics concentration

Indian Journal of Experimental Biology (2023)

Neha Kumari Manju Modgil

Optimization of efficient shoot regeneration protocol and selection of transformed plants at optimum level of antibioticsare pre-requisite in genetic engineering. Here, we explored standardization of in vitro shoot regeneration protocol from leafexplants of apple cv. Oregon Spur for the first time using different concentrations of plant growth regulators. Further, wedetermined the effect of two antibiotics (kanamycin and cefotaxime) on shoot regeneration efficiency. About 95% of leavesinduced adventitious shoots on MS medium supplemented with 4 mg/L BA and 0.2 mg/L NAA. The frequency of shootregeneration increased to 100% when leaf explants were cultured on 2 mg/L TDZ along with 0.5 mg/L NAA. The number ofshoots regenerated per leaf was enhanced but abnormal and vitrified shoots were obtained in case of TDZ supplementedmedium. Dark incubation of initial one week decreased the shoot regeneration frequencies at all the combinations of plantgrowth regulators. Leaf explants were found highly sensitive to kanamycin and inhibited shoot induction above 5 mg/L.This concentration was found suitable for selection of transgenic shoots. Cefotaxime at 100-300 mg/L promotedorganogenesis, whereas 500 mg/L was found optimal to eliminate Agrobacterial overgrowth after co-cultivation with leafexplants. Our results have demonstrated that the above protocol can successfully be applied for transgenic development in‘Oregon Spur’ cultivar of apple in future trials.

Kanamycin

10.56042/ijeb.v61i06.1945

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CRISPR/Cas system for the traits enhancement in potato (Solanum tuberosum L.): present status and future prospectives

Journal of Plant Biochemistry and Biotechnology (2024)

Neha Kumari Abhishek Kumar Shashikant Sharma Palvi Thakur Sanjay Chadha

Solanum tuberosum

Plant biochemistry

10.1007/s13562-024-00878-0

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Effect of weed management practices on growth of cabbage (Brassica oleracea L. var. Capitata.)

Ecology Environment and Conservation (2024)

Dharam Pal SS Lakhawat Brij Gopal Chhipa KK Yadav Hemant Swami

This study evaluated the effect of weed management practices on cabbage. A field experiment was conducted during early spring 2023 at the horticulture farm, Rajasthan College of Agriculture, MPUAT, Udaipur. The result showed that the growth characteristics was significantly affected by Pendimethalin @ 700 gha-1+1 Hand weeding at 45DAT. The highest plant height, number of unwhirled leaves, minimum days taken to head initiation and head harvest was observed in Pendimethalin @ 700 gha-1+1 Hand weeding at 45DAT. All of the above parameters were lowest where weedicide was applied i.e., control.

Capitata

10.53550/eec.2024.v30i04.036

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Estimation of Hormonal Seed Treatments on Enzyme Activities after Accelerated Ageing (Artificial Ageing) in Chickpea (Cicer arietinum L.)

Legume Research - An International Journal (2022)

Sachchida Nand Mishra Neha Kumari Namo Narayan Mishra Kalpana Mishra

Background: Accelerated ageing, which is known to diminish seed viability and vigour in different seed crops, has been used to predict seed storability. The goal of this study was to see how accelerated ageing affected enzymatic characters of chickpea seeds (Variety Pusa 362). Seeds were exposed to accelerated ageing conditions by being heated to 35oC and 40oC and kept at 90% and 100% relative humidity at different duration i.e., 2, 4 and 8 days. After accelerating ageing pre sowing seed treatment with salicylic acid and gibberellic acid has done. The findings revealed that faster ageing causes the chickpea seed to degenerate, which is linked to a decline in enzymatic characters but hormonal seed treatments enhances enzymatic (amylase, catalase, peroxidase, dehydrogenase and protease) performance after ageing. Our result showed that antioxidant activity of aged seeds increased after seed treatment with plant growth hormones. Methods: The present experiment was conducted at state seed testing laboratory, Department of Genetics and Plant Breeding, SHUATS, Prayagraj, Uttar Pradesh during the year 2018 to 2020. In the experiment treatment were comprises with 37 treatment combination. The seeds are exposed to high temperatures and relative humidity in a chamber to speed up the ageing process. After that aged seed were invigorated with hormones i.e., hormonal seed priming. Result: Our finding revealed that the aged seed treated with gibberellic acid and salicylic acid enhanced the activity of antioxidant enzymes which help in seed germination.

Gibberellic acid

Seed treatment

10.18805/lr-4796

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Development of an efficient in vitro regeneration system in broccoli (Brassica oleracea L. var. italica), a highly recommended vegetable crop

Plant Physiology Reports (2023)

Palvi Thakur Neha Kumari Sanjay Chadha

Cotyledon

Petiole (insect anatomy)

Vermiculite

Perlite

10.1007/s40502-023-00739-3

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Impact of Salicylic and Gibberellic Acid Seed Invigoration on Seed Health Status under (Artificial Ageing) Accelerated Ageing Test in Lentil (Lens culinaris Medik.)

Legume Research - An International Journal (2022)

Neha Kumari Sachchida Nand Mishra A. K. Chaurasia

Background: Research examination led during the Rabi season of 2018-19 and 2019-20 to discover the Effect of hormonal seed strengthening on seed health status under accelerated ageing test in lentil (Lens culinaris Medik.) at state seed testing laboratory. The Blotter test was conducted in CRD plan with four replications. From the examination it was demonstrated that the hormonal treatment with salicylic acid at RH (90%) and temperature (35°C) for 2 days ageing showed least infection, while contagious contamination higher with the increment in relative humidity, temperature and duration of artificial ageing. Methods: During the years 2018 to 2020, the current experiment was carried out at the state seed testing laboratory, Department of Genetics and Plant Breeding, SHUATS, Prayagraj, Uttar Pradesh. In the experiment, 37 different treatment permutations were used. To accelerate the ageing process, the seeds were placed in an ageing chamber with high temperatures and relative humidity. The aged seeds was then revitalized with hormones, a process known as hormonal seed fortification. Result: Our finding revealed that the aged seeds treated with gibberellic acid and salicylic acid (Growth Hormone) enhanced the quality of seed health status in Lentil.

Gibberellic acid

Test weight

10.18805/lr-4948

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Recent advances in development and utilization of double haploids (DHs) in economically important vegetable crops

Plant Cell Tissue and Organ Culture (PCTOC) (2023)

Palvi Thakur Neha Kumari Abhishek Kumar Pratibha Sharma Sanjay Chadha

Doubled haploidy

Molecular breeding

Plant Breeding

10.1007/s11240-023-02617-0

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Citations (4)