Tiansheng Shen

The Ohio State University

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Martin F. Schneider

Friedrich-Alexander-Universität Erlangen-Nürnberg

Gene P. Siegal

University of Alabama at Birmingham

Shi Wei

University of Alabama at Birmingham

Yewei Liu

Jackson Laboratory

Zaibo Li

The Ohio State University

Anil V. Parwani

The Ohio State University

William R. Randall

University of Maryland, Baltimore

Jacques Hanoune

Institut National de Recherche en Santé Publique

Yosuke Suzuki

Kanazawa University

Nicole Defer

Hospital Clínic de Barcelona

Cooperative Institutions

The Ohio State University

The University of Texas MD Anderson Cancer Center

University of Alabama at Birmingham

Wayne State University

MSD K.K. (Japan)

The Ohio State University Wexner Medical Center

Henry Ford Health System

Cleveland Clinic

Merck & Co., Inc., Rahway, NJ, USA (United States)

Memorial Sloan Kettering Cancer Center

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Adrenergic signalling between rat taste receptor cells

The Journal of Physiology (2002)

Scott Herness Fangli Zhao Namik Kaya Shao‐gang Lu Tiansheng Shen

In taste buds, synaptic transmission is traditionally thought to occur from taste receptor cells to the afferent nerve. This communication reports the novel observation that taste receptor cells respond to adrenergic stimulation. Noradrenaline application inhibited outward potassium currents in a dose-dependent manner. This inhibition was mimicked by the beta agonist isoproterenol and blocked by the beta antagonist propranolol. The alpha agonists clonidine and phenylephrine both inhibited the potassium currents and elevated intracellular calcium levels. Inwardly rectifying potassium currents were unaffected by adrenergic stimulation. Experiments using the RT-PCR technique demonstrate that lingual epithelium expresses multiple alpha (alpha1a, alpha1b, alpha1c, alpha1d, alpha2a, alpha2b, alpha2c) and beta (beta1, beta2) subtypes of adrenergic receptors, and immunocytochemistry localized noradrenaline to a subset of taste receptor cells. Collectively, these data imply strongly that adrenergic transmission within the taste bud may play a paracrine role in taste physiology.

Taste bud

Taste receptor

Phenylephrine

10.1113/jphysiol.2002.020438

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Citations (63)

TRPS1 Expression in Breast Carcinomas

The American Journal of Surgical Pathology (2021)

Bryce Parkinson Wei Chen Tiansheng Shen Anil V. Parwani Zaibo Li

TRPS1 has been recently demonstrated as a highly sensitive and specific marker for breast carcinomas. To further explore TRPS1's utility in breast carcinoma, we systematically evaluated TRPS1 expression on tissue microarrays from 160 estrogen receptor (ER)-positive/human epidermal growth factor receptor 2 (HER2)-positive, 94 ER-/HER2+, 117 triple-negative breast carcinomas, and 618 other primary carcinomas (cholangiocarcinoma, endometrial, colorectal, and hepatocellular carcinomas), and whole tissue sections from 64 HER2+, 76 triple-negative, and 67 metaplastic breast carcinomas. The results showed TRPS1 was highly expressed in breast carcinomas (100% of HER2+ and 97.4% of triple negative on whole tissue sections), but almost completely negative in other tested tumor types. TRPS1 was also highly expressed in metaplastic carcinoma (91%), significantly higher than GATA3 (55.2%). The different expression between TRPS1 and GATA3 was most prominent in chondroid/mesenchymal subtypes (100% vs. 36.4%), followed by spindle cell carcinoma (66.7% vs. 44.4%). In addition, TRPS1 was expressed in normal breast ductal epithelial cells with less staining than in carcinoma cells, and TRPS1 showed aberrant membranous staining in HER2+ breast carcinomas that suggests a potential cross-reactivity with HER2 protein.

Tissue microarray

Breast carcinoma

Metaplastic Carcinoma

GATA3

10.1097/pas.0000000000001824

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Citations (39)

α‐Adrenergic signalling activates protein kinase D and causes nuclear efflux of the transcriptional repressor HDAC5 in cultured adult mouse soleus skeletal muscle fibres

The Journal of Physiology (2009)

Yewei Liu Minerva Contreras Tiansheng Shen William R. Randall Martin F. Schneider

The protein kinase PKD1 has recently been linked to slow fibre‐type gene expression in fast skeletal muscle through phosphorylation of class II histone deacetylase (HDAC) molecules, resulting in nuclear efflux of HDAC and consequent activation of the transcription factor MEF2. However, possible upstream activators of PKD, and the time course and signalling pathway of downstream effectors have not been determined in skeletal muscle. Using fluorescent fusion proteins HDAC5–green fluorescent protein (GFP) and PKD1–mPlum expressed in fibres isolated from predominantly slow soleus muscle and maintained for 4 days in culture, we now show that α‐adrenergic receptor activation by phenylephrine causes a transient, PKD‐dependent HDAC5–GFP nuclear efflux. Concurrent to this response, PKD1–mPlum transiently redistributes from cytoplasm to plasma membrane and nuclei, and back, during 2 h exposure to phenylephrine. The recovery may reflect α‐receptor desensitization. In contrast, the phorbol ester PMA (phorbol‐12‐myristate‐13‐acetate, a pharmacological mimic of the downstream mediator diacylglycerol in α‐adrenergic signalling), caused continuous PKD‐dependent HDAC5–GFP nuclear efflux and maintained PKD1–mPlum redistribution. In the absence of expressed HDAC, PMA increased histone H3 acetylation and increased MEF2 reporter activity in a PKD‐dependent manner, consistent with PKD phosphorylation of endogenous HDAC(s) and reduced nuclear HDAC activity due to HDAC nuclear efflux. HDAC5–GFP did not respond to PMA in fibres from predominantly fast flexor digitorum brevis (FDB) muscle, but did in FDB fibres expressing exogenous PKD1. Our results demonstrate that a PKD‐mediated signalling pathway for HDAC nuclear efflux is activated in slow skeletal muscle through adrenergic input, which is typically active in parallel with motor neurone input during muscular activity.

Histone deacetylase 5

10.1113/jphysiol.2008.164566

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Citations (18)

The CD4/CD8 ratio of tumor-infiltrating lymphocytes at the tumor-host interface has prognostic value in triple-negative breast cancer

Human Pathology (2017)

Kai Wang Tiansheng Shen Gene P. Siegal Shi W

Triple-negative breast cancer

Tumor-infiltrating lymphocytes

Breast tumor

10.1016/j.humpath.2017.09.012

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Citations (97)

An Indoor positioning method based on Improved Elman neural network using sparrow search

2022 7th International Conference on Communication, Image and Signal Processing (CCISP) (2022)

Tiansheng Shen Wenying Chen Binbin Zhu Yan Wang Yongliang Zhou

N owadays, indoor positioning algorithms have attracted comprehensive attention and research. Due to multipath effect in the complex indoor environment, it is difficult to position with high precision. In this paper a BLE(Bluetooth Low Energy) indoor positioning algorithm based on ISSA(Improved Sparrow Search Algorithm) with Cat chaotic mapping is proposed. Firstly the abnormal data are removed by BDOR(Bilateral Direction Outlier Removal) algorithm before establishing the database of the collected BLE data. Then the rest of data are filtered by improved Kalman filter. The ISSA-Elman models are studied to predict the horizontal and vertical coordinates of the point to be tested. Results of experiments reveal that the proposed algorithm performs precisely in indoor positioning. The minimal position error is nearly 2cm.

Position (finance)

Indoor positioning system

10.1109/ccisp55629.2022.9974419

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Citations (0)

Characterization of estrogen receptor–negative/progesterone receptor–positive breast cancer

Human Pathology (2015)

Tiansheng Shen Margaret Brandwein‐Gensler Omar Hameed Gene P. Siegal Shi Wei

Progesterone receptor

10.1016/j.humpath.2015.07.019

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Citations (41)

Activity- and Calcineurin-independent Nuclear Shuttling of NFATc1, but Not NFATc3, in Adult Skeletal Muscle Fibers

Molecular Biology of the Cell (2006)

Tiansheng Shen Yewei Liu Zoltán Cseresnyés Arie Hawkins William R. Randall

The transcription factor NFATc1 may be involved in slow skeletal muscle gene expression. NFATc1 translocates from cytoplasm to nuclei during slow fiber type electrical stimulation of skeletal muscle fibers because of activation of the Ca(2+)-dependent phosphatase calcineurin, resulting in nuclear factor of activated T-cells (NFAT) dephosphorylation and consequent exposure of its nuclear localization signal. Here, we find that unstimulated adult skeletal muscle fibers exhibit a previously unanticipated nucleocytoplasmic shuttling of NFATc1 without appreciable nuclear accumulation. In resting fibers, the nuclear export inhibitor leptomycin B caused nuclear accumulation of NFATc1 (but not of isoform NFATc3) and formation of NFATc1 intranuclear bodies independent of calcineurin. The rate of nuclear uptake of NFATc1 was 4.6 times lower in resting fibers exposed to leptomycin B than during electrical stimulation. Inhibitors of glycogen synthase kinase and protein kinase A or of casein kinase 1 slowed the decay of nuclear NFATc1 after electrical stimulation, but they did not cause NFATc1 nuclear uptake in unstimulated fibers. We propose that two nuclear translocation pathways, one pathway mediated by calcineurin activation and NFAT dephosphorylation and the other pathway independent of calcineurin and possibly independent of NFAT dephosphorylation, determine the distribution of NFATc1 between cytoplasm and nuclei in adult skeletal muscle.

NFAT

Dephosphorylation

Nuclear export signal

10.1091/mbc.e05-08-0780

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Citations (43)

Adenylyl cyclases: structure, regulation and function in an enzyme superfamily

Molecular and Cellular Endocrinology (1997)

Jacques Hanoune Yves Pouille Eleni T. Tzavara Tiansheng Shen Larissa Lipskaya

Homeostasis

Sympathetic nervous system

10.1016/s0303-7207(97)04013-6