Elena A. Prokopyeva

Federal Research Center of Fundamental and Translational Medicine

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i-10 index

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Kirill Sharshov

Federal Research Center of Fundamental and Translational Medicine

АМ

А. М. Шестопалов

Federal Research Center of Fundamental and Translational Medicine

Ivan Sobolev

Federal Research Center of Fundamental and Translational Medicine

Olga Kurskaya

Federal Research Center of Fundamental and Translational Medicine

АY

А. Yu. Alekseev

Dagestan State University

Andrei A. Yarushkin

Federal Research Center of Fundamental and Translational Medicine

Vladimir O. Pustylnyak

Novosibirsk State University

Tatyana Murashkina

Federal Research Center of Fundamental and Translational Medicine

Yuliya A. Pustylnyak

Novosibirsk State University

Anastasiya Derko

Federal Research Center of Fundamental and Translational Medicine

Cooperative Institutions

Federal Research Center of Fundamental and Translational Medicine

Siberian Branch of the Russian Academy of Sciences

Russian Academy of Sciences

Novosibirsk State University

Research Institute of Influenza

All-Russian Research Institute for Animal Health

Ministry of Health of the Russian Federation

Siberian Research Institute of Agriculture and Peat

Institute of Chemical Biology and Fundamental Medicine

Academy of Medical Sciences

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Pathology of A(H5N8) (Clade 2.3.4.4) Virus in Experimentally Infected Chickens and Mice

Interdisciplinary Perspectives on Infectious Diseases (2019)

Elena A. Prokopyeva Vsevolod Zinserling You-Chan Bae Yong‐Kuk Kwon Olga Kurskaya

The emergence of novel highly pathogenic avian influenza viruses (HPAIVs) in migratory birds raises serious concerns as these viruses have the potential to spread during fall migration. We report the identification of novel HPAIV A(H5N8) clade 2.3.4.4 virus that was isolated from sick domestic duck at commercial farm during the second wave of spread that began in October and affected poultry (ducks; chiсkens) in several European regions of Russia and Western Siberia in 2016. The strain was highly lethal in experimental infection of chickens and mice with IVPI = 2.34 and MLD50 = 1.3log10⁡ EID50, accordingly. Inoculation of chickens with the HPAIV A/H5N8 demonstrated neuroinvasiveness, multiorgan failure, and death of chickens on the 3rd day post inoculation. Virus replicated in all collected organ samples in high viral titers with the highest titer in the brain (6.75±0.1 log10TCID50/ml). Effective virus replication was found in the following cells: neurons and glial cells of a brain; alveolar cells and macrophages of lungs; epithelial cells of a small intestine; hepatocytes and Kupffer cells of a liver; macrophages and endothelial cells of a spleen; and the tubular epithelial cells of kidneys. These findings advance our understanding of histopathological effect of A(H5N8) HPAIV infection.

10.1155/2019/4124865

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Citations (10)

Constitutive androstane receptor activation evokes the expression of glycolytic genes

Biochemical and Biophysical Research Communications (2016)

Andrei A. Yarushkin Yuliya A. Kazantseva Elena A. Prokopyeva Diana N. Markova Yuliya A. Pustylnyak

Constitutive androstane receptor

PKM2

10.1016/j.bbrc.2016.08.075

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Citations (9)

Adaptation of influenza A(H1N1)pdm09 virus in experimental mouse models

Infection Genetics and Evolution (2016)

Elena A. Prokopyeva Ivan Sobolev M.V. Prokopyev А. М. Шестопалов

Lethality

10.1016/j.meegid.2016.01.022

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Citations (25)

Characterization and Phylodynamics of Reassortant H12Nx Viruses in Northern Eurasia

Microorganisms (2019)

Kirill Sharshov Junki Mine Ivan Sobolev Olga Kurskaya Nikita Dubovitskiy

Wild waterfowl birds are known to be the main reservoir for a variety of avian influenza viruses of different subtypes. Some subtypes, such as H2Nx, H8Nx, H12Nx, and H14Nx, occur relatively rarely in nature. During 10-year long-term surveillance, we isolated five rare H12N5 and one H12N2 viruses in three different distinct geographic regions of Northern Eurasia and studied their characteristics. H12N2 from the Far East region was a double reassortant containing hemagglutinin (HA), non-structural (NS) and nucleoprotein (NP) segments of the American lineage and others from the classical Eurasian avian-like lineage. H12N5 viruses contain Eurasian lineage segments. We suggest a phylogeographical scheme for reassortment events associated with geographical groups of aquatic birds and their migration flyways. The H12N2 virus is of particular interest as this subtype has been found in common teal in the Russian Far East region, and it has a strong relation to North American avian influenza virus lineages, clearly showing that viral exchange of segments between the two continents does occur. Our results emphasize the importance of Avian Influenza Virus (AIV) surveillance in Northern Eurasia for the annual screening of virus characteristics, including the genetic constellation of rare virus subtypes, to understand the evolutionary ecology of AIV.

Reassortment

Viral phylodynamics

Lineage (genetic)

Nucleoprotein

10.3390/microorganisms7120643

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Citations (8)

Experimental Infection of Adapted Influenza B Virus in Mice Model

Elena A. Prokopyeva Olga Kurskaya Ivan Sobolev Mariia Solomatina Tatyana Murashkina

Over the years influenza B virus (IBV) contribute annual disease and can lead to serious respiratory disease among humans. More attention should be paid to the mammalian adaptive processes of B viruses and development of vaccines against current influenza. Because of preclinical trials of anti-influenza drugs are conducted mainly on mice, we developed adequate animal model using antigenically-relevant IBV strain for testing anti-influenza drugs and protective efficacy of flu vaccines. We serially passaged Victoria lineage (clade 1A) IBV 17 times in BALB/c mice. The adaptive amino acid substitutions were found in HA (T214I) and NA (D432N). By the electron microscopic examination, we showed spherical and elliptical shapes of IBV. Light microscopy showed that mouse-adapted B virus caused influenza pneumonia on day 6 post inoculation. We evaluated the illness pathogenicity, viral load and histopathological features of mouse-adapted IBV and estimated anti-influenza drugs and vaccine efficiency in vitro and in vivo. Assessment of investigational anti-influenza drug oseltamivir ethoxisuccinate and flu vaccine Ultrix® revealed effectivity against our mouse-adapted influenza B virus.

Oseltamivir

Strain (injury)

10.20944/preprints202002.0022.v1

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Citations (5)

Low incidence of human coronavirus among hospitalized children in Novosibirsk city, Russia during pre-pandemic period (2013–2020)

Journal of Microbiology Immunology and Infection (2021)

Olga Kurskaya Elena A. Prokopyeva Angelika V. Anoshina Natalya V. Leonova Olga A. Simkina

We investigated the incidence of 15 respiratory viruses among 2991 children with acute respiratory infections in Novosibirsk city, Russia, prior to the COVID-19 pandemic (2013-2020). Viral infections were detected in 72.5% cases. The incidence of human coronavirus was 2% (Alphacoronaviruses, 63%; Betacoronaviruses, 37%).

Pandemic

Coronavirus

2019-20 coronavirus outbreak

Betacoronavirus

10.1016/j.jmii.2021.07.013

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Citations (6)

Etiology of influenza-like illnesses in the population of Novosibirsk city in the 2018–2019 epidemic season

Russian Journal of Infection and Immunity (2021)

Olga Kurskaya Angelika V. Anoshina Natalya V. Leonova Olga A. Simkina Tatyana V. Komissarova

Influenza and other acute respiratory viral infections lead to a substantial incidence of severe cases and hospitalizations and so remain a global health problem. Within the frame of the Global Influenza Hospital Surveillance Network (GIHSN), we assessed the contribution of influenza and other respiratory viruses to severe cases of influenzalike diseases in patients hospitalized to the Novosibirsk infectious hospitals in the years 2018–2019. We analyzed 484 nasopharyngeal swabs collected from patients admitted to the hospitals with acute respiratory infections (ARI) using real-time polymerase chain reaction commercial kits. We confirmed viral etiology of ARI in 69.8% cases. Influenza viruses were detected in 47.1% cases, wherein concomitant circulation of influenza A(H1N1)pdm09 and A(H3N2) viruses was observed in 20.7% and 26% of patients, respectively, whereas influenza B virus was detected only in one sample. All analyzed influenza A viruses were antigenically similar to vaccine strains. Genetically, the Novosibirsk strains were closely related to influenza A viruses distributed in Russia and worldwide. Influenza A(H1N1)pdm09 virus was detected in all patients aged 0 to 14 years and required intensive care. Other respiratory viruses were detected in 36.4% of children and 5.8% of adults, and 8.3% of children had viral coinfection, whereas no cases of coinfection were detected in adults. The most common viruses in children were metapneumovirus — 12.8%, rhinovirus — 9.3% and respiratory syncytial virus — 8.0%. In adults, metapneumovirus, adenovirus, parainfluenza virus and rhinovirus were detected with a detection rate no exceeding 2%. In this study, we found no differences in the detection rate of the influenza virus due to concomitant chronic diseases, pregnancy, or smoking habits. At the same time, the detection rate of other respiratory viruses in non-smokers vs. smokers was significantly lower than in smokers and former smokers (26.15%, 66.67% and 62.50%, respectively). In addition, the level of detection of respiratory viruses in children with vs. without chronic pathology was significantly higher (55.3% and 38.7%, respectively). Thus, our and similar studies are important for monitoring and control of the infection.

Rhinovirus

Etiology

10.15789/2220-7619-eoi-1439

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Citations (3)

Virological and Genetic Characterization of the Unusual Avian Influenza H14Nx Viruses in the Northern Asia

Viruses (2023)

Nikita Dubovitskiy Anastasiya Derko Ivan Sobolev Elena A. Prokopyeva Tatyana Murashkina

Wild aquatic birds are generally identified as a natural reservoir of avian influenza viruses (AIVs), where a high diversity of subtypes has been detected. Some AIV subtypes are considered to have relatively low prevalence in wild bird populations. Six-year AIV surveillance in Siberia revealed sporadic cases of the rarely identified H14-subtype AIV circulation. Complete genome sequencing of three H14 isolates were performed, and the analysis indicated interconnections between low pathogenic avian influenza (LPAI) viruses. We conducted hemagglutination inhibition and virus neutralization assays, estimated the susceptibility of isolates to neuraminidase inhibitors, and characterized receptor specificity. Our study revealed circulation of a new H14N9 subtype described for the first time. However, the low prevalence of the H14-subtype AIV population may be the reason for the underestimation of the diversity of H14-subtype AIVs. According to the available data, a region in which H14-subtype viruses were detected several times in 2007–2022 in the Eastern Hemisphere is Western Siberia, while the virus was also detected once in South Asia (Pakistan). Phylogenetic analysis of HA segment sequences revealed the circulation of two clades of H14-subtype viruses originated from initial 1980s Eurasian clade; the first was detected in Northern America and the second in Eurasia.

10.3390/v15030734

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Citations (1)

ACCELERATOR MASS-SPECTROMETRY FOR DETECTING ULTRA-LOW CONCENTRATIONS OF VIRAL PARTICLES, LABELLED BY RADIOCARBON

Траектория исследований – человек природа технологии (2022)

Elena A. Prokopyeva Е. В. Пархомчук

Accelerator mass spectrometry

10.56564/27825264_2022_1_112

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Citations (1)

VARIABILITY OF THE INFLUENZA A VIRUS

South of Russia ecology development (2016)

Ivan Sobolev Olga Kurskaya Kirill Sharshov Elena A. Prokopyeva А. Yu. Alekseev

Aim. To investigate the mechanisms of variability of the influenza virus. Discussion. Influenza is one of the most significant problems facing the health care system. Human influenza causes a number of social and economic problems: mortality in high-risk groups, increasing in the load on personnel agencies health and reduced manpower. Features of the influenza virus genome enable the pathogen to cause seasonal epidemics each year, despite the preventive measures (vaccination) and using of specific antiviral drugs. Conclusion. Variability of the influenza virus genome is provided by antigenic drift, antigenic shift and recombination. This mechanisms of the variability of influenza virus that allow to remain a highly contagious infectious agent able to infect a large number of host species and cause significant problems as a result of epidemics and pandemics.

Pandemic

Antigenic shift

Viral phylodynamics

H5N1 genetic structure

Antigenic variation

10.18470/1992-1098-2016-1-170-177

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Citations (3)