Kengo Sasaki

Kobe University

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Akihiko Kondo

Kobe University

Daisuke Sasaki

The University of Tokyo

Masahiko Morita

RIKEN Center for Advanced Photonics

Chiaki Ogino

Kobe University

Daisuke Sasaki

Nagasaki University Hospital

Yota Tsuge

Kanazawa University

Yasuo Igarashi

Southwest University

Naoya Ohmura

Central Research Institute of Electric Power Industry

Hideo Kawaguchi

Hirosaki University

Tomohisa Hasunuma

Kobe University

Cooperative Institutions

Kobe University

Tianjin Hi-Tech Superconducting Electronic Technology (China)

The University of Tokyo

Osaka University

Kyoto University

Gorgias Press (United States)

Tohoku University

Nagoya University

Japan Science and Technology Agency

Hokkaido University

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<i>In vitro</i> Evaluation of Prebiotics Materials and Taurine in a Batch Human Colonic Microbiota Model System

JOURNAL OF THE BREWING SOCIETY OF JAPAN (2018)

Kengo Sasaki

近年，ヒト腸内細菌が宿主の健康に多大な影響を及ぼしていることが明らかとなってきた。同時に，食品中に存在して有益菌の増殖を促し，腸内環境を整える成分が注目されてきた。本稿においては食品の機能性評価を行い，有用菌が腸内環境に与える効果を簡便に評価することが可能であるin vitro培養系ヒト大腸菌叢モデルについて紹介いただいた。本モデルを利用して，機能性食品の開拓や有益菌の発見へと展開されることが期待される。

10.6013/jbrewsocjapan.113.71

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Differences in glucose yield of residues from among varieties of rice, wheat, and sorghum after dilute acid pretreatment

Figshare (2017)

Hiroshi Teramura Kengo Sasaki Hideo Kawaguchi Fumio Matsuda Jun Kikuchi

Bio-refinery processes require use of the most suitable lignocellulosic biomass for enzymatic saccharification and microbial fermentation. Glucose yield from biomass solid fractions obtained after dilute sulfuric acid (1%) pretreatment (at 180 °C) was investigated using 14, 8, and 16 varieties of rice, wheat, and sorghum, respectively. Biomass solid fractions of each crop showed similar cellulose content. However, glucose yield after enzymatic hydrolysis (cellulase loading at 6.6 filter paper unit/g-biomass) was different among the varieties of each crop, indicating genotypic differences for rice, wheat, and sorghum. Nuclear magnetic resonance method revealed that the high residual level of lignin aromatic regions decreased glucose yield from solid fraction of sorghum.

10.6084/m9.figshare.5114506

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P871 Successful detection of dysbiosis and altered short-chain-fatty acids levels in in vitro colonic microbiota culture system using faecal samples of ulcerative colitis

Journal of Crohn s and Colitis (2018)

Jun Inoue Kengo Sasaki Namiko Hoshi Daisuke Sasaki Akihiko Kondo

Ulcerative colitis (UC) involves chronic and recurring inflammation of the intestinal tract. It is widely recognised that the composition of gut microbiota and intestinal metabolites are altered in UC patients. There is increasing number of clinical studies which supports the idea that imbalance of gut microbiota (dysbiosis) plays a role in pathogenesis and/or progression of UC. We previously reported single–batch fermentation system (Takagi et al., PLoS One, 2016) which can approximately recapitulate the microbiota of fecal sample in vitro, and could detect the change of microbiota and metabolites when prebiotics were supplemented to the system. It prompted us to test if this system could actually detect the unbalanced fecal microbiota and metabolites of UC patients; which may suggest the potential usefulness of this system for preclinical evaluation of effect of probiotics and prebiotics before administration to patients. Fecal samples were obtained from 11 UC patients and 13 healthy human subjects (HS). Fecal samples were applied to the single–batch fermentation system and aliquots of fermentation cultures were sampled to analyse the composition of bacteria and short-chain-fatty acids (SCFAs) concentration. Bacterial 16S rRNA gene sequencing was performed with Illumina MiSeq. Concentrations of SCFAs were measured using a high-performance liquid chromatograph. Bacterial component and SCFAs concentration were compared between UC and HS. The bacterial diversity and richness in fecal samples were maintained in corresponding fermentation cultures of both UC and HS. Comparison of principal coordinate plots of the unweighted UniFrac distance showed that compositional difference of microbiota between HS and UC patients were successfully reproduced in the single–batch fermentation system as observed in faces. Relative abundance of family Lachnospiraceae, which includes butyrate producing species, was significantly decreased in the microbiota of UC patients compared with those of HS, which was the same tendency as observed in faces. Butyrate generation was significantly decreased in the colon culture models of UC patients compared with those of HS and strongly correlated with relative abundance of family Lachnospiraceae. In this study, our new culture system could successfully recapitulate the previously shown dysbiosis of reduced family Lachnospiraceae and reduced butyrate level in UC patients in vitro. It may have a potential to be used as an in vitro evaluation system to check the intestinal environment in health and disease. It could be a good model to test whether dysbiosis can be corrected into symbiosis by adding prebiotics and/or probiotics to modify the gut microbiota community in each UC patient.

Dysbiosis

10.1093/ecco-jcc/jjx180.998

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Bioelectrochemical regulation accelerates facultatively syntrophic proteolysis

Journal of Bioscience and Bioengineering (2012)

Daisuke Sasaki Kengo Sasaki Masahiko Morita Shin-ichi Hirano Norio Matsumoto

Methanogen

Proteolysis

10.1016/j.jbiosc.2012.02.013

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Sucrose purification and repeated ethanol production from sugars remaining in sweet sorghum juice subjected to a membrane separation process

Applied Microbiology and Biotechnology (2017)

Kengo Sasaki Yota Tsuge Hideo Kawaguchi Masahiro Yasukawa Daisuke Sasaki

Sweet sorghum

Ultrafiltration (renal)

Ethanol Fermentation

10.1007/s00253-017-8316-3

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2P-2031 Analyses of thermophilic bioelectrochemical methanogenic reactor including a supporting material

日本生物工学会大会講演要旨集 (2010)

Daisuke Sasaki Kengo Sasaki Masahiko Morita Shin-ihci Hirano Norio Matsumoto

Source

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Comparison of metabolomic profiles of microbial communities between stable and deteriorated methanogenic processes

Bioresource Technology (2014)

Daisuke Sasaki Kengo Sasaki Yota Tsuge Masahiko Morita Akihiko Kondo

Tricarboxylic acid

10.1016/j.biortech.2014.08.054

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Efficient production of methane from artificial garbage waste by a cylindrical bioelectrochemical reactor containing carbon fiber textiles

AMB Express (2013)

Daisuke Sasaki Kengo Sasaki Atsushi Watanabe Masahiko Morita Yasuo Igarashi

A cylindrical bioelectrochemical reactor (BER) containing carbon fiber textiles (CFT; BER + CFT) has characteristics of bioelectrochemical and packed-bed systems. In this study, utility of a cylindrical BER + CFT for degradation of a garbage slurry and recovery of biogas was investigated by applying 10% dog food slurry. The working electrode potential was electrochemically regulated at -0.8 V (vs. Ag/AgCl). Stable methane production of 9.37 L-CH4 · L-1 · day-1 and dichromate chemical oxygen demand (CODcr) removal of 62.5% were observed, even at a high organic loading rate (OLR) of 89.3 g-CODcr · L-1 · day-1. Given energy as methane (372.6 kJ · L-1 · day-1) was much higher than input electric energy to the working electrode (0.6 kJ · L-1 · day-1) at this OLR. Methanogens were highly retained in CFT by direct attachment to the cathodic working electrodes (52.3%; ratio of methanogens to prokaryotes), compared with the suspended fraction (31.2%), probably contributing to the acceleration of organic material degradation and removal of organic acids. These results provide insight into the application of cylindrical BER + CFT in efficient methane production from garbage waste including a high percentage of solid fraction.

Carbon fibers

Degradation

Biodegradable waste

Biogas

10.1186/2191-0855-3-17

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Table of Contents

IEEE Transactions on Applied Superconductivity (2020)

M Mangiarotti L.-C. Duda G Fiscarelli Mike Kirby D Bajko

Table (database)

10.1109/tasc.2020.2994844

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2P282 Chimeric proton-pumping rhodopsins containing the third cytoplasmic loop of bovine rhodopsin(The 48th Annual Meeting of the Biophysical Society of Japan)

Seibutsu Butsuri (2010)

Kengo Sasaki Takahiro Yamashita Aya Nakatsuma Keiichi Inoue Yoshinori Shichida

EmeAFMteS61vV7VtnF7)y"ImodeEffrecoanM IA1520Role ef aromatic residue for inter-molecuNar interaction between bacteriorhodepsin trimer stodied b.v high-speed AFM H"yHto 1'amashita.MikihiT'o ShibaLa.Taknyuki LTchihashi, 'lbshio Ando, (DeFartment of PSt)'sics, Kana:.thva(Jnii,ersity)

10.2142/biophys.50.s132_4

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