Kazuto Ogura

Tokyo Metropolitan Institute of Gerontology

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Takashi Ikeda

Shizuoka Cancer Center

Fumihiko Kimura

National Defense Medical College

Ken Sato

Teikyo University

Kazuo Motoyoshi

National Defense Medical College

Hiroki Torikai

The University of Texas MD Anderson Cancer Center

Shinichi Kobayashi

National Defense Medical College

Tomoaki Akagi

Nagasaki University Hospital

Ayako Kobayashi

St. Marianna University School of Medicine

Hisao Imai

Saitama Medical University

Kohmei Kubo

Aomori Prefectural Central Hospital

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National Defense Medical College

Tokyo Metropolitan Institute of Gerontology

Tokyo Metropolitan Geriatric Hospital

Hirosaki University

Aomori Prefectural Central Hospital

National Defense Medical College Hospital

The University of Tokyo

Kyoto Prefectural University of Medicine

Kyoto University

Tohoku University

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Abnormal neutrophil scattergram obtained using Pentra MS CRP in the patients with myelodysplastic syndrome showing dysgranulopoiesis

International Journal of Laboratory Hematology (2015)

Tohru Inaba K. Ishizuka Soichi Yuasa N. Mishima Kazuto Ogura

Summary Introduction Pentra MS CRP is an automated hematology analyzer capable of cytochemistry using Chlorazol black E, a lipid‐staining agent, for white blood cell (WBC) differentials. Pentra MS CRP displays a WBC scattergram according to the cell volume obtained using flow impedance and light absorbance reflecting the Chlorazol black E (CBE)‐positive lipid content. Method Neutrophil scattergrams obtained using Pentra MS CRP were compared between 5 patients with myelodysplastic syndrome (MDS) and normal controls. Sudan black B (SBB)‐staining patterns of peripheral blood neutrophils were subdivided into four types (types I, II, III, and VI) based on their staining intensity and scored by counting 200 cells. Such SBB scores were also compared between the two groups. Results Neutrophil scattergrams deviated downward in the MDS group, suggesting the decreased CBE positivity that seemed reflect the reduction of the lipid content in dysplastic neutrophils. SBB scores determined in this study were also decreased in the MDS group when compared with those in normal controls. Conclusion Pentra MS CRP might rapidly generate useful information on dysplastic neutrophils in patients with MDS based on its cytochemistry for WBC differentials during routine laboratory hematology.

Hematology analyzer

Sudan Black B

Cytochemistry

Hematology

White blood cell

10.1111/ijlh.12423

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