The quantum yield of photosynthetic O(2) exchange was measured in eight species of leaf succulents representative of both malic enzyme type and phosphoenolpyruvate carboxykinase type CAM plants. Measurements were made at 25 degrees C and CO(2) saturation using a leaf disc O(2) electrode system, either during or after deacidification. The mean quantum yield was 0.095 +/- 0.012 (sd) moles O(2) per mole quanta, which compared with 0.094 +/- 0.006 (sd) moles O(2) per mole quanta for spinach leaf discs measured under the same conditions. There were no consistent differences in quantum yield between decarboxylation types or during different phases of CAM metabolism. On the basis of current notions of compartmentation of CAM biochemistry, our observations are interpreted to indicate that CO(2) refixation is energetically independent of gluconeogenesis during deacidification.

10.1104/pp.81.1.297

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Citations (30)

CO<sub>2</sub> fixation in leaves of a CAM plant without lower epidermis and the effect of CO<sub>2</sub> on their deacidification

Plant and Cell Physiology (1977)

Kojiro Nishida

Journal Article CO2 fixation in leaves of a CAM plant without lower epidermis and the effect of CO2 on their deacidification Get access Kojiro Nishida Kojiro Nishida Botanical Institute, Faculty of Science, Kanazawa UniversityKanazawa, Japan Search for other works by this author on: Oxford Academic Google Scholar Plant and Cell Physiology, Volume 18, Issue 4, August 1977, Pages 927–930, https://doi.org/10.1093/oxfordjournals.pcp.a075508 Published: 01 August 1977 Article history Received: 03 February 1977 Published: 01 August 1977

Epidermis (zoology)

10.1093/oxfordjournals.pcp.a075508

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Citations (10)

Photoswelling and light-inactivation of isolated chloroplasts II. Functional and structural changes in isolated chloroplasts under the influence of lysolecithin

Plant and Cell Physiology (1975)

Satoshi Hoshina Kojiro Nishida

10.1093/oxfordjournals.pcp.a075163

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Citations (13)

Carbonic Anhydrase in the Marine Alga Ulva pertusa

Physiologia Plantarum (1968)

Masahiko Ikemori Kojiro Nishida

Abstract The preparation of stable samples containing carbonic anhydrase from Ulva pertusa was achieved by using artificial sea water and 10 m M cysteine solution adjusted to pH 8.2 instead of 10 m M cysteine solution alone. When the sample was stored at 0°C in darkness the enzyme activity was retained for about 24 h. In contrast to the results obtained with land plants in previous works a remarkable inhibitory effect of low concentrations of sulfanilamide and Diamox (2‐acetylamino‐1,3,4‐thiadiazole‐5‐sulfonamide) on the activity of the enzyme was observed.

Sulfanilamide

Sulfonamide

Darkness

10.1111/j.1399-3054.1968.tb07252.x

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Citations (17)

LIGHT-INDUCED HIGH-AMPLITUDE SWELLING AND SHRINKING IN ISOLATED SPINACH CHLOROPLASTS

Plant and Cell Physiology (1966)

Kojiro Nishida Naoto Tamai Kazuo Ryoyama

It was found that light accelerates the swelling of spinach chloroplasts suspended in a solution containing NaCl and Tris-HCl, and that, in about 60 min, the swollen chloroplasts begin to shrink rapidly. The volume of chloroplasts reached, on swelling, 270 per cent of the original volume on the average and then shrank to 17 per cent. The rate of swelling and the maximum volume were affected markedly by the tonicity of NaCl. NH4Cl did not affect the volume changes, while phenazine methosulfate accelerated the swelling remarkably. The relationship between photoshrinkage and this light-induced high-amplitude swelling was discussed.

10.1093/oxfordjournals.pcp.a079193

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Citations (5)

Acid-base induced calcium uptake by spinach chloroplasts

Plant and Cell Physiology (1969)

Kojiro Nishida Y. Hayashi

Journal Article Acid-base induced calcium uptake by spinach chloroplasts Get access KOJIRO NISHIDA, KOJIRO NISHIDA Botanical Institute, Faculty of Science, Kanazawa UniversityKanazawa, Japan Search for other works by this author on: Oxford Academic Google Scholar YOSHIYUKI HAYASHI YOSHIYUKI HAYASHI Botanical Institute, Faculty of Science, Kanazawa UniversityKanazawa, Japan Search for other works by this author on: Oxford Academic Google Scholar Plant and Cell Physiology, Volume 10, Issue 4, December 1969, Pages 751–757, https://doi.org/10.1093/oxfordjournals.pcp.a074461 Published: 01 December 1969 Article history Received: 12 March 1969 Published: 01 December 1969

10.1093/oxfordjournals.pcp.a074461

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Citations (0)

On the balloon-formation of isolated chloroplasts and grana

Experientia (1965)

Kojiro Nishida T. Hayashi

Nitella

10.1007/bf02138484

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Citations (2)

Relationships between CO₂ concentration within the leaf and photosynthesis during deacidification in a CAM plant, Kalanchoë pinnatum

Physiologia Plantarum (1982)

Kojiro Nishida

The CO 2 concentration within the leaf of Kalanchoë pinnatum (Persoon) was measured during deacidification in the light. When the acidified leaf was treated with DCMU, the CO 2 concentration within the leaf was increased about 3‐fold as compared to that of the non‐treated leaf, concomitant with a decline in deacidification. Low light intensity during deacidification also increased CO 2 concentration within the leaf. From these experiments, it was concluded that in order for deacidification to proceed, CO 2 released from malate must be fixed continuously by photosynthesis. Otherwise, an equilibrium for malate decomposition is soon established and results in suppression of deacidification. Thus, the CO 2 concentration within the leaf seems to be one of the regulatory factors of deacidification.

DCMU

10.1111/j.1399-3054.1982.tb00707.x

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Citations (9)

Effects of Mineral Salts on Short-term Incorporation of Carbon Dioxide inChlorella

Journal of Experimental Botany (1959)

Osmund Holm‐Hansen Kojiro Nishida V. Moses M. Calvin

The effects of various mineral salts have been studied, both singly and in combination, on the uptake and distribution of photosynthetically incorporated carbon-14 in Chlorella. When magnesium sulphate, potassium nitrate, and potassium phosphate were combined in the same concentrations as are found in the normal nutrient solution, the uptake of carbon-14 was increased and more radioactivity was incorporated into the amino-acids, with a decrease in the percentage of radioactivity in the sugar phosphates when compared to the controls in distilled water. The most striking effect of the addition of just one salt was obtained with salts containing nitrogen, particularly ammonium chloride, which in one experiment increased the radioactivity found in the amino-acids from 9.9 per cent. (expressed as percentage of the total soluble radioactivity found in this group of compounds) in the controls to 57 per cent., while the radioactivity in the sugar phosphates fell from 64 to 7 per cent., correspondingly. The total uptake of carbon dioxide by the cells supplied with ammonium chloride was three times as great as that in the controls in distilled water. The relevance of these findings to the methodology involved in experiments dealing with the path of carbon in photosynthesis is discussed, with particular emphasis upon the concept of a 'steady state' and how it is thought this may best be achieved.

Distilled water

Carbon fibers

Total inorganic carbon

10.1093/jxb/10.1.109

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Citations (43)