Heloísa Sobreiro Selistre-de-Araújo

Universidade Federal de São Carlos

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Co-Authors

Rita de Cássia Marqueti

Universidade de Brasília

Márcia R. Cominetti

Universidade Federal de São Carlos

Bianca Cruz Pachane

Universidade Federal de São Carlos

Wanessa Fernanda Altei

Hospital de Câncer de Barretos

Charlotte L. Ownby

Oklahoma State University

Dulce Helena Ferreira de Souza

Universidade Federal de São Carlos

Javier Ellena

Universidade Federal de São Carlos

Uliana Sbeguen Stotzer

Universidade Federal de São Carlos

Alzir A. Batista

Universidade Federal de São Carlos

Patty Karina dos Santos

Universidade Federal de São Carlos

Cooperative Institutions

Universidade Federal de São Carlos

367

Universidade de São Paulo

275

Universidade Estadual Paulista (Unesp)

Universidade Estadual de Campinas (UNICAMP)

Universidade de Ribeirão Preto

Universidade Federal do Rio de Janeiro

Universidade de Brasília

Universidade Federal de São Paulo

Universidade Federal de Uberlândia

Centro Universitário de Araraquara

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An ex vivo and in vitro investigation of extracellular vesicle interactions with B cells of Macaca nemestrina and humans

bioRxiv (Cold Spring Harbor Laboratory) (2025)

Bianca Cruz Pachane Blanca V. Rodriguez Erin N. Shirk Олеся Гололобова Bess Carlson

Extracellular vesicles may modify recipient cell behavior through multiple mechanisms, including interacting with the cell surface or internal membrane components and delivering luminal cargo to the cytoplasm. Here, we use a previously established ex vivo approach to investigate the cellular fate of EVs spiked into whole blood samples from nonhuman primate (NHP) and human donors and contrast these findings with results from in vitro assays. We report that EVs are internalized by NHP and human B cells while also associating to some degree with other PBMCs. EVs exhibit greater association with B cells in ex vivo whole blood compared to isolated B cells, suggesting that blood components may promote EV interactions or that cell isolation factors may inhibit this association. Cellular uptake of EVs involves clathrin-dependent endocytosis and may be aided by other pathways, including direct EV-cell membrane fusion. Overall, our data suggest that EV association, including uptake, by B cells occurs in at least two primate species. These findings highlight the potential to develop new strategies to either enhance or inhibit EV tropism toward B cells.

Ex vivo

Extracellular vesicles

10.1101/2025.02.12.637883

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Citations (0)

Construção de modelos tridimensionais de metaloproteinases do veneno da serpente Agkistrodon contortrix laticinctus através de técnicas de modelagem molecular

E Sonoda Heloísa Sobreiro Selistre-de-Araújo Ignez Caracelli Glaucius Oliva

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Oxidative stress biomarkers of exposure in the blood of cichlid species from a metal-contaminated river

Ecotoxicology and Environmental Safety (2007)

Clarissa Bonafé Gaspar Ruas Cleoni dos Santos Carvalho Heloísa Sobreiro Selistre-de-Araújo Evaldo Luiz Gaeta Espíndola Marisa Narciso Fernandes

Cichlid

Oreochromis mossambicus

10.1016/j.ecoenv.2007.08.018

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Citations (191)

Characterization of the penicillin G acylase from Bacillus megaterium ATCC 14945

Brazilian Archives of Biology and Technology (2005)

Vanessa Ribeiro de Souza Ana C. G. Silva Laura Marina Pinotti Heloísa Sobreiro Selistre-de-Araújo Raquel de Lima Camargo Giordano

The purpose of this work was to characterize the enzyme penicillin G acylase (PGA) produced by Bacillus megaterium. Purification of the enzyme by ultra/diafiltration did not allow the detection of the PGA band by SDS-PAGE electrophoresis due to the high content of remaining proteins. However, using the DNA of the microorganism, it was possible to replicate the genes of the two B. megaterium PGA reported in literature, showing that the enzyme consisted of two sub-units, having 245 and 537 amino acids each and an average molecular mass of 26950 and 59070 Da, respectively. The parameters studied were: 1) the influence of temperature in the 25-60(0)C range, 2) pH in the 5-10 range and 3) substrate concentration, this was tested to obtain results on the Penicillin G hydrolysis reaction rate, using the initial velocities approach. The maximum hydrolysis rate was obtained at 37ºC and pH 8.0. The Michaelis-Menten model fitted well, resulting in estimated Km and Vmax parameters values of 1.83 mM and 0.165*10-3 mmol/min/UI, respectively.

Bacillus megaterium

Molecular mass

Michaelis–Menten kinetics

10.1590/s1516-89132005000400013

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Citations (27)

Tumour but not stromal expression of β3 integrin is essential, and is required early, for spontaneous dissemination of bone‐metastatic breast cancer

The Journal of Pathology (2014)

Rachel Zoe Carter Kelli Cristina Micocci Anthony Natoli Richard P. Redvers Sophie Paquet‐Fifield

Although many preclinical studies have implicated β3 integrin receptors (αvβ3 and αIIbβ3) in cancer progression, β3 inhibitors have shown only modest efficacy in patients with advanced solid tumours. The limited efficacy of β3 inhibitors in patients could arise from our incomplete understanding of the precise function of β3 integrin and, consequently, inappropriate clinical application. Data from animal studies are conflicting and indicate heterogeneity with respect to the relative contributions of β3-expressing tumour and stromal cell populations in different cancers. Here we aimed to clarify the function and relative contributions to metastasis of tumour versus stromal β3 integrin in clinically relevant models of spontaneous breast cancer metastasis, with particular emphasis on bone metastasis. We show that stable down-regulation of tumour β3 integrin dramatically impairs spontaneous (but not experimental) metastasis to bone and lung without affecting primary tumour growth in the mammary gland. Unexpectedly, and in contrast to subcutaneous tumours, orthotopic tumour vascularity, growth and spontaneous metastasis were not altered in mice null for β3 integrin. Tumour β3 integrin promoted migration, protease expression and trans-endothelial migration in vitro and increased vascular dissemination in vivo, but was not necessary for bone colonization in experimental metastasis assays. We conclude that tumour, rather than stromal, β3 expression is essential and is required early for efficient spontaneous breast cancer metastasis to bone and soft tissues. Accordingly, differential gene expression analysis in cohorts of breast cancer patients showed a strong association between high β3 expression, early metastasis and shorter disease-free survival in patients with oestrogen receptor-negative tumours. We propose that β3 inhibitors may be more efficacious if used in a neoadjuvant setting, rather than after metastases are established.

10.1002/path.4490

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Citations (41)

A novel αvβ3-blocking disintegrin containing the RGD motive, DisBa-01, inhibits bFGF-induced angiogenesis and melanoma metastasis

Clinical & Experimental Metastasis (2007)

Oscar Henrique Pereira Ramos Alexandre Kauskot Márcia R. Cominetti Iga Bechyne Carmen Lucia Salla Pontes

Alpha-v beta-3

Matrigel

10.1007/s10585-007-9101-y

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Citations (94)

Snake Venom Metalloproteinases (SVMPs): A structure-function update

Toxicon X (2020)

Olamide Tosin Olaoba Patty Karina dos Santos Heloísa Sobreiro Selistre-de-Araújo Dulce Helena Ferreira de Souza

Snake venom metalloproteinases (SVMPs) represent a diverse group of multi-domain proteins with several biological activities such as the ability to induce hemorrhage, proteolytic degradation of fibrinogen and fibrin, induction of apoptosis and inhibition of platelet aggregation. Due to these activities, SVMPs are responsible for many of the well-known pathological phenotypes in snake envenomations caused particularly by species from the Viperidae family and the Crotalinae subfamily. These proteins have been classified based on their size and domain structure into P-I, P-II and P-III classes. Comparatively, members of the P-I SVMPs possess the simplest structures, formed by the catalytic metalloproteinase domain only; the P-II SVMPs are moderately more complex, having the canonical disintegrin domain in addition to the metalloproteinase domain; members of the P-III class are more structurally varied, comprising the metalloproteinase, disintegrin-like, and cysteine-rich domains. Proteolytic cleavage, repeated domain loss and presence of other ancillary domains are responsible for structural diversities in the P-III class. However, studies continue to unveil the relationship between the structure and function of these proteins. In this review, we recovered evidences from literature on the structural peculiarities and functional classification of Snake Venom Metalloproteinases. In addition, we reflect on diversities that exist among each class while taking into account specific and up-to-date class-based activities.

Viperidae

10.1016/j.toxcx.2020.100052

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Citations (91)

Biomechanical responses of different rat tendons to nandrolone decanoate and load exercise

Scandinavian Journal of Medicine and Science in Sports (2010)

Rita de Cássia Marqueti Jonato Prestes C. C. Wang Oscar Henrique Pereira Ramos Sergio Perez

Androgenic‐anabolic steroids (AAS) have been associated with an increased incidence of tendon rupture. The aim of this study was to compare the biomechanical properties of the rat calcaneal tendon (CT), superficial flexor tendon (SFT), and deep flexor tendon (DFT), and to determine the effect of jump training in association with AAS. Animals were separated into four groups: sedentary, trained, AAS‐treated sedentary rats (AAS), and AAS‐treated and trained animals. Mechanical testing showed that the CT differed from the DFT and SFT, which showed similar mechanical properties. Jump caused the CT to exhibit an extended toe region, an increased resistance to tensional load, and a decreased elastic modulus, characteristics of an elastic tendon capable of storing energy. AAS caused the tendons to be less compliant, and the effects were reinforced by simultaneous training. The DFT was the most affected by training, AAS, and the interaction of both, likely because of its involvement in the toe‐off step of jumping, which we suggest is related to the rapid transmission of force as opposed to energy storage. In conclusion, tendons are differently adapted to exercise, but responded equally to AAS, showing reduced flexibility, which is suggested to increase the risk of tendon rupture in AAS consumers.

Anabolic-Androgenic Steroids

Biomechanics

10.1111/j.1600-0838.2010.01162.x

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Citations (29)

Platelet a disintegrin and metallopeptidase 10 expression correlates with clock drawing test scores in Alzheimer's disease

International Journal of Geriatric Psychiatry (2013)

Patrícia Regina Manzine Elizabeth Joan Barham Francisco Assis Carvalho Vale Heloísa Sobreiro Selistre-de-Araújo Sofía Cristina Iost Pavarini

Objective Earlier studies have demonstrated that a disintegrin and metallopeptidase 10 (ADAM10) levels are reduced in Alzheimer's disease (AD) patients compared with healthy subjects. The objective of this study was to evaluate whether platelet ADAM10 levels correlates with the clock drawing test (CDT) scores, which is a simple and a reliable measure of visuospatial ability and executive function in AD patients. Methods Thirty elderly patients with probable AD and 25 healthy patients forming the control group, matched by age, gender, and educational level, were evaluated. Platelet proteins were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis, and ADAM10 was identified by western blotting. The Spearman's correlation coefficient between ADAM10 and CDT was obtained for each group. The areas under the curves were used to compare the receiver operating characteristic curves. Results The CDT scores and platelet ADAM10 expression were significantly different between patients with AD and controls and also along the disease's progression. In AD patients, there was a positive correlation between ADAM10 expression and CDT scores. Among non‐AD subjects, no correlation was found. The combination of ADAM10 and CDT was significantly better to confirm the AD diagnosis than the AUCs of ADAM10 and CDT separately. Conclusions The association of blood‐based biomarkers, such as ADAM10, and cognitive tests may be helpful for a more reliable AD diagnosis. Copyright © 2013 John Wiley & Sons, Ltd.

ADAM10

10.1002/gps.4020

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Citations (32)

Molecular Cloning and Sequence Analysis of cDNAs for Metalloproteinases from Broad-Banded Copperhead Agkistrodon contortrix laticinctus

Archives of Biochemistry and Biophysics (1995)

Heloísa Sobreiro Selistre-de-Araújo Charlotte L. Ownby

Cloning (programming)

10.1006/abbi.1995.1352

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Citations (38)