Shixiang Wu

Yangtze University

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Hualin Yang

Yangtze University

Yu Zhou

Yangtze University

Xingping Zhang

Gannan Medical University

Guohao Zhang

Second Hospital of Shanxi Medical University

Yanan Zhao

Tohoku University

Wang Chen

Beijing Chaoyang Emergency Medical Center

Yao Xiao

Liaoning Normal University

Peiwu Li

Oil Crops Research Institute

Dapeng Peng

Huazhong Agricultural University

Wang Chen

Ministry of Agriculture and Rural Affairs

Cooperative Institutions

Yangtze University

Huazhong Agricultural University

State Key Laboratory of Agricultural Microbiology

Ministry of Agriculture and Rural Affairs

Chinese Academy of Agricultural Sciences

Chinese Academy of Sciences

Tianjin Medical University

Southwest University of Science and Technology

China Agricultural University

Qingdao University of Science and Technology

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Cerium Ions Triggered Dual-Readout Immunoassay for Fluorescent and Colorimetric Detection of Ochratoxin a

SSRN Electronic Journal (2022)

Wang Chen Xingping Zhang Qian Zhang Guohao Zhang Shixiang Wu

Ochratoxin A (OTA) is one of the most prevalent and toxic mycotoxins. Ultrasensitive and convenient detection of OTA is urgent demanded for public health. In this work, a dual-readout immunoassay was established for the detection of OTA based on Ce 4+ oxidizing 3,3′,5,5′-tetramethylbenzidine (TMB) and Ce 3+ inducing aggregation induced emission (AIE) of Au nanoclusters (AuNCs). Under alkaline phosphatase (ALP), the ascorbic acid 2-phosphate (AA2P) can form ascorbic acid (AA) by dephosphorylation. The AA can reduce Ce 4+ to generate Ce 3+ , which induced the AIE of AuNCs to enhance the fluorescence intensity of AuNCs. Meanwhile, unreacted Ce 4+ oxidized TMB to form blue oxTMB. Thus, a dual-readout immunoassay was developed based on AIE of AuNCs and TMB as substrate. The limits of detection (LODs) were as low as 0.62 ng/mL for fluorescent assay and 0.81 ng/mL for colorimetric assay. The recoveries of OTA from corn were 94.4%-107.7% for the fluorescent mode and 93.7%-106.9% for the colorimetric mode. The results verified that the cerium ions triggered dual-readout immunoassay was reliable to sensitive detect OTA in corn samples.

10.2139/ssrn.4121316

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Dual-readout immunoassay based on IFE and p-phenylenediamine for detection of zearalenone

Journal of Food Composition and Analysis (2024)

Shixiang Wu Yanan Zhao Yao Xiao Liyuan Ma Qi Zhang

o-Phenylenediamine

10.1016/j.jfca.2024.106311

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Citations (1)

Ratiometric fluorescence immunoassay based on silver nanoclusters and calcein-Ce3+ for detecting ochratoxin A

Talanta (2023)

Yanan Zhao Wang Chen Huajuan Fang Junxiang Zhang Shixiang Wu

Calcein

Nanoclusters

Bovine serum albumin

Ochratoxins

10.1016/j.talanta.2023.125470

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Citations (9)

Rapid immunoassays for the detection of quinoxalines and their metabolites residues in animal-derived foods: A review

Food Chemistry (2024)

Liangni Qin Jiaxu Xiao Hongfei Yang Jixiang Liang Long Li

Hapten

10.1016/j.foodchem.2024.138539

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Citations (4)

MnO2 nanosheets-triggered oxVB1 fluorescence immunoassay for detection zearalenone

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy (2022)

Guohao Zhang Xingping Zhang Qian Zhang Wang Chen Shixiang Wu

10.1016/j.saa.2022.121954

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Citations (12)

Alkaline phosphatase triggered ratiometric fluorescence immunoassay for detection of zearalenone

Food Control (2022)

Shixiang Wu Xingping Zhang Wang Chen Guohao Zhang Qian Zhang

10.1016/j.foodcont.2022.109541

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Citations (15)

Cerium ions triggered dual-readout immunoassay based on aggregation induced emission effect and 3,3′,5,5′-tetramethylbenzidine for fluorescent and colorimetric detection of ochratoxin A

Analytica Chimica Acta (2022)

Wang Chen Xingping Zhang Qian Zhang Guohao Zhang Shixiang Wu

10.1016/j.aca.2022.340445

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Citations (26)

Ratiometric Fluorescence Immunoassay Based on Silver Nanoclusters and Calcein-Ce <sup>3 </sup> for Detecting Ochratoxin a

Yanan Zhao Wang Chen Huajuan Fang Junxiang Zhang Shixiang Wu

Ochratoxin A (OTA), a dangerous mycotoxin, is found in many crops. It is essential to create sensitive OTA detection techniques to ensure food safety. Based on the principle of p-nitrophenol (PNP) quenched the fluorescence of bovine serum albumin silver nanocluster (BSA-AgNCs) through an internal filtering effect, and phosphate activated fluorescence of calcein-Ce3+ system, a ratiometric fluorescence immunoassay for OTA detection was developed. In this strategy, the value of F518/F640 was used as a signal for response of OTA concentration. The detection range of this strategy was 0.625–25 ng/mL, the limit of detection (LOD) was 0.04 ng/mL. This new immunoassay offered a brand-new platform for detecting OTA.

Nanoclusters

Calcein

10.2139/ssrn.4564602

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Dual-Readout Immunoassay Based on IFE and <i>P</i>-Phenylenediamine for Detection of Zearalenone

Shixiang Wu Yanan Zhao Yao Xiao Liyuan Ma Qi Zhang

In this study, we present a straightforward dual-readout immunoassay for sensitive and specific detection of zearalenone (ZEN) using the inner filter effect (IFE) and p-phenylenediamine (PPD). 2-aminoterephthalic acid (PTA-NH2) is an organic compound that exhibits strong fluorescence at 420 nm. In the presence of horseradish peroxidase (HRP) and hydrogen peroxide (H2O2), PPD was oxidized to 2,5-diamino-N, N'-bis(p-aminophenyl)-1,4-benzoquinone di-imine (PPDox). The fluorescence of PTA-NH2 was quenched due to the IFE effect, resulting from the effective overlap between the UV-vis absorption spectra of PPDox and the fluorescence spectra of PTA-NH2. Furthermore, PPDox was completely oxidized to p-benzoquinone (PBQ) by sulfuric acid, exhibiting a strong absorption peak at 415 nm and a yellow color. Consequently, a dual-readout immunoassay based on IFE and PPD was developed for the detection of ZEN. The detection limits for the fluorescent and colorimetric modes were 0.012 ng/mL and 0.048 ng/mL, respectively. The approach can be used to efficiently and accurately detect ZEN in real samples due to the high sensitivity, specificity, and consistency of the dual-readout modes.

10.2139/ssrn.4641155

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Development of time-resolved fluorescence immunochromatography and ic-ELISA based on monoclonal antibodies specifically recognizing fumonisin B1

Food Bioscience (2024)

Xiaoming Pan Yalin Yang Huaming Li Jiaxu Xiao Linwei Zhang

Fumonisin

10.1016/j.fbio.2024.104131

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Citations (3)