Farnaz Kouhestani

Tehran University of Medical Sciences

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Saeed Reza Motamedian

Shahid Beheshti University of Medical Sciences

Sadra Mohaghegh

Shahid Beheshti University of Medical Sciences

Nima Ahmadi

Shahid Beheshti University of Medical Sciences

Parisa Motie

Isfahan University of Medical Sciences

Maryam Rezai Rad

Shahid Beheshti University of Medical Sciences

Fatemeh Alirezaei

Babol University of Medical Sciences

Milad Soleimani

Alborz University of Medical Sciences

Behzad Hooshmand

Shahid Beheshti University of Medical Sciences

Mehrnoosh Hasan Shahriari

Amirkabir University of Technology

Z Kadkhoda

Tehran University of Medical Sciences

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Shahid Beheshti University of Medical Sciences

Tehran University of Medical Sciences

Shahid Beheshti University

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University of Tehran

Baqiyatallah University of Medical Sciences

Isfahan University of Medical Sciences

Hamedan University of Medical Sciences

Shahed University

Tarbiat Modares University

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Comparison of 0.2% chlorhexidine mouthwash with and without anti-discoloration system in patients with chronic periodontitis: A randomized controlled clinical trial

Journal of Advanced Periodontology & Implant Dentistry (2019)

Siamak Yaghobee Farid Abedin Dorkoosh Farnaz Kouhestani Ghazaleh Mozafari Hoori Aslroosta

Background. Chemical plaque control, an adjunct to mechanical approaches, could improve the maintenance of patients with different types of periodontitis. Chlorhexidine, the gold standard in chemical plaque control, might have some side effects; the most determining one is tooth discoloration. Anti-discoloration systems (ADS) have been added to minimize brownish tooth discoloration. This study aimed to evaluate the staining potential and clinical efficacy of chlorhexidine with and without ADS in patients with chronic periodontitis. Methods. In this randomized controlled trial, 46 patients with chronic periodontitis were randomly allocated to two groups. Each patient used 10 mL of mouthwash A (CHX without ADS) or B (CHX with ADS, including sodium metabisulfite and ascorbic acid) twice a day for two weeks. After a two-week interval, they used the second mouthwash. At the beginning and the end of each two-week cycle, plaque index (PI), bleeding on probing (BoP), and staining index were recorded. Results. There was no significant difference between mouthwash A and B in the reduction of BoP and PI. The staining index was significantly lower after rinsing with mouthwash B compared to mouthwash A. Conclusion. CHX mouthwash containing ADS has similar efficacy in microbial plaque control and reduction of BOP as CHX without ADS, with the advantage of lower stain formation on tooth surfaces in patients with chronic periodontitis.

Bleeding on probing

Tooth discoloration

Stain

10.15171/japid.2019.011

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Efficacy of Application of Periodontal Ligament Stem Cells in Bone Regeneration: A Systematic Review of Animal Studies

Dental Hypotheses (2022)

Pardis Aghandeh Farnaz Kouhestani Fatemeh Isamorad Solmaz Akbari Behrad Tanbakuchi

Introduction: We aimed to systematically review the animal studies that have investigated the efficacy of periodontal ligament stem cells (PDLSCs) in bone regeneration. Methods: Web of science, Scopus, PubMed, Embase, Cochrane Library, and ProQuest were searched to September 2022 for animal studies investigating bone regeneration using PDLSCs. Results: Twenty studies were included. Calvaria and alveolar defects were treated with stem cells. The cells were mainly carried with hydrogel, hydroxyapatite, and collagen scaffolds. PDLSCs promoted osteogenesis compared with cell-free scaffolds, except in one study where biphasic calcium phosphate block scaffolds alone or with Bone morphogenic protein 2 had superior outcomes in calvaria defects. Controversial results were reported when comparing the osteogenic potential of PDLSCs and bone marrow stem cells. Two studies reported higher potential in BMSCs, and two others reported higher bone formation and more bone quality in PDLSCs. Conclusion: With the limitations of this study, PDLSCs might have promising potential to accelerate bone regeneration in artificial defects; however, due to high heterogeneity in the outcomes of the present studies, before moving forward to human experiments, further preclinical in vivo studies are needed.

Calvaria

Periodontal fiber

10.4103/denthyp.denthyp_136_22

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Failure Rate of Pediatric Dental Treatment under General Anesthesia

Dentistry Journal (2018)

Effat Khodadadi Mehrnaz Mohammadpour Saeed Reza Motamedian Farnaz Kouhestani

Aim: To assess the failure rates of various pediatric dental treatments performed under general anesthesia (GA) after six months to five years of follow-up. Design: This multicenter retrospective cohort study was performed on patients treated by five pedodontists in two private hospitals located in northern Iran during 2010⁻2013 and comprised 155 patients. The patients were recalled and clinically examined. During the clinical examination of the primary teeth, oral hygiene, dmft index, and failure of previous treatments was evaluated. The data were analyzed using the Chi square and regression analyses with a significance level of 0.05. Results: 114 patients (74 males and 40 females, mean age: 37.17 ± 10.75 months) with 1155 primary teeth treated under GA participated in the follow-up. The overall failure rate was 6.59%. The failure rates of pulpectomy, pulopotomy, fissure sealant, stainless steel crown (SSC), amalgam, and composite fillings were 2.90%, 3.03%, 4.83%, 5.26%, 5.33%, and 9.63%, respectively. Among the confounding factors, only gender had a significant effect on the anterior composite failure rate (p = 0.029) and age had a significant effect on the failure rate of fissure sealant therapy (p = 0.015) and SSC (p = 0.018). Conclusion: The overall rate of treatment failure in pediatric patients, treated under GA, was 6.59%.

Pulpectomy

Amalgam (chemistry)

10.3390/dj6030025

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Effect of mechanical forces on the behavior of osteoblasts: a systematic review of in vitro studies

Dental and Medical Problems (2023)

Parisa Motie Sadra Mohaghegh Farnaz Kouhestani Saeed Reza Motamedian

Mechanical loading can play a critical role in bone modeling/remodeling through osteoblasts, with several factors being involved in this process.The present study aims to systematically review the effect of mechanical stimulation on human osteoblast cell lineage combined with other variables.The PubMed and Scopus databases were electronically searched for studies analyzing the effect of compression and tension on human osteoblasts at different differentiation stages. Studies that used carcinogenic osteoblasts were excluded. In addition, studies that did not analyze the osteogenic differentiation or proliferation of cells were excluded. The risk of bias of the studies was evaluated using the modified CONSORT (Consolidated Standards of Reporting Trials) checklist. a total of 20 studies were included. The cells were subjected to tension and compression in 5 and 15 studies, respectively. The application of uniaxial and cyclic strain increased the proliferation of osteoblasts. The same increased pattern could be observed for the osteogenesis of the cells. The impact of the tensile force on the expression of the osteoclastic markers differed based on the loading characteristics. On the other side, the impact of compression on the proliferation of osteoblasts varied according to the magnitude and duration of the force. Besides, different patterns of alternations were observed among the osteogenic markers in response to compression. Meanwhile, compression increased the expression of the osteoclastic markers. It has been shown that the response of the markers related to bone formation or resorption can be altered based on the differentiation stage of the cells, the cell culture system, and the magnitude and duration of the force.

10.17219/dmp/151639

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Comparison of Periodontal Ligament Stem Cells with Mesenchymal Stem Cells from other Sources: A Scoping Systematic Review of In-vitro and In-vivo Studies

Current Stem Cell Research & Therapy (2022)

Z Kadkhoda Parisa Motie Maryam Rezaei Rad Sadra Mohaghegh Farnaz Kouhestani

Objective: The application of stem cells in regenerative medicine depends on their biological properties. This scoping review aimed to compare the features of periodontal ligament stem cells (PDLSSCs) with stem cells derived from other sources. Design: An electronic search in PubMed/Medline, Embase, Scopus, Google Scholar and Science Direct was conducted to identify in vitro and in vivo studies limited to English language. Results: Overall, 65 articles were included. Most comparisons were made between bone marrow stem cells (BMSCs) and PDLSCs. BMSCs were found to have lower proliferation and higher osteogenesis potential in vitro and in vivo than PDLSCs; on the contrary, dental follicle stem cells and umbilical cord mesenchymal stem cells (UCMSCs) had a higher proliferative ability and lower osteogenesis than PDLSCs. Moreover, UCMSCs exhibited a higher apoptotic rate, hTERT expression, and relative telomerase length. The immunomodulatory function of adipose-derived stem cells and BMSCs was comparable to PDLSCs. Gingival mesenchymal stem cells showed less sensitivity to long-term culture. Both pure and mixed gingival cells had lower osteogenic ability compared to PDLSCs. Comparison of dental pulp stem cells (DPSCs) with PDLSCs regarding proliferation rate, osteo/adipogenesis, and immunomodulatory properties was contradictory; however, in vivo bone formation of DPSCs seemed to be lower than PDLSCs. Conclusion: In light of the performed comparative studies, PDLSCs showed comparable results to stem cells derived from other sources; however, further in vivo studies are needed to determine the actual pros and cons of stem cells in comparison to each other.

Regenerative Medicine

Periodontal fiber

10.2174/1574888x17666220429123319

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Epstein-Barr virus reactivation and DNA damage response

Farnaz Kouhestani

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Effect of metformin on the behavior of dental pulp stem cells cultured on freeze-dried bone allografts

Dental and Medical Problems (2021)

Farnaz Kouhestani Maryam Rezai Rad Sadra Mohaghegh Saeed Reza Motamedian

Background.Considering the complications associated with autogenous bone grafting, the use of freezedried bone allograft (FDBA) granules may be considered as an alternative treatment plan.Objectives.The aim of this study was to evaluate the effect of metformin on both the proliferation and osteogenic capability of dental pulp stem cells (DPSCs) cultured on FDBA granules. Material and methods.First, a pilot study was conducted only on DPSCs to confirm cellular viability and the osteoinducing effect of 100 μmol/L metformin.Next, the cells were loaded on FDBA granules and treated with and without metformin.Finally, the following analyses were performed: scanning electron microscopy (SEM) (cell attachment); the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (proliferation); and alkaline phosphatase (ALP) activity analysis (osteogenic differentiation).Results.The SEM images revealed that metformin enhanced the adhesion of DPSCs on FDBA granules.In addition, metformin was shown to increase cell proliferation/viability from day 1 to day 7. Compared to the control, a significant difference was observed after 7 days of treatment.Metformin enhanced the osteogenic capability of FDBA in both standard and osteoinducing conditions.An increase in ALP activity was significant after 7 days of treatment.The positive effect of metformin on differentiation was significant in osteoinducing conditions.Conclusions.Metformin can be applied as an additional osteoinductive factor in bone regeneration treatment.Moreover, scaffolds with controlled release of metformin can be considered a proper osteoinductive bone substitute that may lessen the complications related to applying allograft scaffolds alone.

MTT assay

Viability assay

10.17219/dmp/131988

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Application of Stem Cells in Consolidation Phase of Distraction Osteogenesis: A Systematic Review and Meta-Analysis

Journal of Maxillofacial and Oral Surgery (2025)

Nima Ahmadi Fatemeh Alirezaei Sadra Mohaghegh Farnaz Kouhestani Saeed Reza Motamedian

10.1007/s12663-025-02434-2

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Detection of Hepatitis C Virus RNA in blood and saliva of transfusion-dependent thalassemia patients diagnosed with Hepatitis C

Contemporary Clinical Dentistry (2018)

Farnaz Kouhestani Behzad Hooshmand Seyed Moayed Alavian Maryam Firouzmandi Saeed Reza Motamedian

The aim of the current study was to detect hepatitis C virus (HCV) RNA in blood and saliva of a population of patients with thalassemia who have HCV antibody in their serum.In this cross-sectional study, blood and saliva samples were collected and were analyzed with quantitative reverse transcription polymerase chain reaction (RT-PCR) for the detection of HCV RNA. In addition, liver-related blood tests were performed, and patients' medical history was recorded. Data were analyzed by independent samples t-test and Chi-square with a significant level of 0.05.Overall, 62 adult patients (29 males and 33 females) were included. Most (87%) of the patients had major thalassemia and genotype 1a was the most common (42%) type. HCV RNA was detected in 71 and 16% of blood and saliva samples, respectively. HCV RNA was detected more in female patients (31%) (P = 0.003) and in intermediate thalassemia (50%) (P < 0.005). The mean age of the patients with positive saliva was almost 10 years older (P < 0.001), and the mean number of blood transfusion was fewer in positive saliva group (P = 0.037). The sensitivity, specificity, and positive and negative predictive values of saliva PCR was calculated to be 18%, 88%, 80%, and 69%, respectively.Saliva contained HCV RNA in 16% of the assessed population. The probability of detection of HCV RNA in saliva increased in older patients, less number of blood transfusions, females and intermediate thalassemia. Saliva RT-PCR demonstrated low sensitivity and high specificity with high positive predictive value in the assessed population.

Hepatitis C

10.4103/ccd.ccd_297_17

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Application of chemical factors for acceleration of consolidation phase of the distraction osteogenesis: a scoping review

Oral and Maxillofacial Surgery (2022)

Sadra Mohaghegh Fatemeh Alirezaei Nima Ahmadi Farnaz Kouhestani Saeed Reza Motamedian

10.1007/s10006-022-01097-4

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Citations (4)