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Tomáš Čajka

Czech Academy of Sciences, Institute of Physiology

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Oliver Fiehn

University of California, Davis

Jana Hajšlová

University of Chemistry and Technology, Prague

Ondřej Kuda

Czech Academy of Sciences, Institute of Physiology

Martin Rossmeisl

Czech Academy of Sciences

Megan R. Showalter

University of California, Davis

Ján Kopecký

Czech Academy of Sciences

Kateřina Riddellová

ALS (Czechia)

Stanley L. Hazen

Cleveland Clinic

W.H. Wilson Tang

Cleveland Clinic

Tomáš Mráček

Czech Academy of Sciences

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Czech Academy of Sciences

124

Charles University

Czech Academy of Sciences, Institute of Physiology

University of California, Davis

University of Chemistry and Technology, Prague

Institute of Clinical and Experimental Medicine

Cleveland Clinic Lerner College of Medicine

Czech Academy of Sciences, Institute of Molecular Genetics

Cleveland Clinic

University of California System

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Gas Chromatography in Food Analysis

CRC Press eBooks (2008)

Jana Hajšlová Tomáš Čajka

10.1201/9781420045673.ch7

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Citations (17)

The artificial sweetener erythritol and cardiovascular event risk

Nature Medicine (2023)

Marco Witkowski Ina Nemet Hassan S. Alamri Jennifer Wilcox Nilaksh Gupta

Erythritol

Artificial Sweetener

Cardiovascular event

10.1038/s41591-023-02223-9

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Citations (110)

Tips and tricks for LC–MS-based metabolomics and lipidomics analysis

TrAC Trends in Analytical Chemistry (2024)

Stanislava Rakusanova Tomáš Čajka

Metabolomics and lipidomics are rapidly growing fields, leading to novel discoveries and advancing the understanding of biological processes at the molecular level. However, designing a proper workflow and choosing from countless options can be challenging, especially for beginners in the field. To address this challenge, we provide a comprehensive overview of metabolomics and lipidomics tools and a step-by-step guide that includes "tips and tricks" based on current metabolomics and lipidomics analysis approaches. We include power analysis, sample collection and preparation, separation and detection of metabolites using primarily liquid chromatography–mass spectrometry (LC–MS), processing of raw instrumental files, quality control, statistical analysis, and data sharing. This guide offers practical insights applicable to diverse research areas, covering all the essential steps in metabolomic and lipidomic profiling.

Profiling (computer programming)

10.1016/j.trac.2024.117940

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Citations (3)

Advances in Mass Spectrometry for Food Authenticity Testing

Elsevier eBooks (2016)

Tomáš Čajka Megan R. Showalter Kateřina Riddellová Oliver Fiehn

10.1016/b978-0-08-100220-9.00007-2

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Citations (16)

Production of 2HG from wild-type or R172K mutant IDH2 transfected cells

Megan R. Showalter Jason Hatakeyama Tomáš Čajka Kacey VanderVorst Kermit L. Carraway