Objective To compare the conventional methods in vector surveillance for Dengue fever in order to search for the best method and evaluating index at frontier port.Methods 7 docks at Fuzhou seaport randomly three months from Aug.2010 to Oct.2010 for vector survey of Dengue fever were choszen.The traditional methods such as labor hour method and larva index and ovitrap and Counting of mosquito larvae in 100 ml of waterand haz ard evaluation of Dengue fever were choszen.Results The traditional methods have some disadvantages in vector surveillance for Dengue fever, especially the most important indexes in judgment and prevention and controlling of dengue fever such as Breteau index and housing index and container index are not suit for surveillance at frontier port,they may underestimate the intensity of vectors at frontier port.Conclusion We should enhance the study of mosquito magnet pro trap and ovitrap,in order to search after the sensitive and differential methods of vector surveillance for Dengue fever at frontier port.
Objective To survey the liver function level and the risk factors in heroin addicts in Wenzhou city. Methods A total of 684 heroin addicts in Drug Rehabilitation Center of Wenzhou city and 717 health persons in Medical Examination Center of Wenzhou city were surveyed contemporaneously. The levels of ALT,AST were measured and compared in both of heroin addicts and healthy cases. Results Comparing with healthy cases,ALT and AST levels,abnormal rate of liver function in heroin addicts were significantly higher during withdrawal period (P0.001). Likewise,ALT and AST levels,abnormal rate of liver function were higher in intravenous drug users than in inhaling drug users. Both of the drug-using time and the daily dose had positive correlation with ALT level in heroin addicts (P0.05). The relationship between blood glucose and ALT level was negative (P0.001). TG,TC and LDL-C had a negative correlation with ALT level (P0.05) and AST level (P0.001),respectively. And the relationship between HDL-C and ALT level and AST level was positive (P0.05). Logistic Regression analysis indicated that drug-using time,intravenous injection and blood glucose were associated with ALT abnormality,while intravenous injection and TG were associated with AST abnormality. Conclusion The transaminase levels and prevalence rates of liver function abnormality were significantly higher in heroin addicts than in normal persons. It may be associated with the long drug-using time,intravenous injection,low BG and low TG.
Background and Aims Biliary atresia is the most common cause of liver disease and liver transplantation in children. The accumulation of bile acids in hepatocytes and the stimulation of the intestinal microbiome can aggravate the disease progression. This study investigated changes in the composition of the gut microbiota and its metabolites in biliary atresia and the possible effects of these changes on disease progression. Methods Stool samples of biliary atresia at different disease stages and matched control individuals were collected (early stage: 16 patients, 16 controls; later stage: 16 patients, 10 controls). Metagenomic sequencing was performed to evaluate the gut microbiota structure. Untargeted metabolomics was performed to detect and analyze the metabolites and bile acid composition. Results A disturbed gut microbiota structure occurred in the early and later stages of biliary atresia. Klebsiella , Streptococcus , Veillonella , and Enterococcus have always been dominant. The abundance of V. atypica displayed significant changes between the early and later stages of biliary atresia. Combined with clinical indicators, Spearman’s analysis showed that Klebsiella and Veillonella atypica strongly correlated with liver enzymes. Enterococcus faecium had an enormously positive relationship with lithocholic acid derivatives. Metabolites involved in tryptophan metabolism were changed in the patients with biliary atresia, which had a significant association with stool V. atypica and blood total bilirubin ( p < 0.05). Conclusions The liver damage of biliary atresia was directly or indirectly exacerbated by the interaction of enriched Klebsiella ( K. pneumoniae ), Veillonella ( V. atypica ), and Enterococcus ( E. faecium ) with dysmetabolism of tryptophan and bile acid.
Taking into consideration its present urban economic standards and population scale, the City of Kunming adopted a development strategy which gives priority to people's needs and public transit, pays enough attention to the environment, and supports Kunming's sustainable development. In accordance with the public-transit-first policy, Kunming successfully rolled out China's first center-lane bus lane during the 1999 World Horticultural Exposition. Recent surveys show that the bus lane is outstanding with regard to both environmental protection and passenger transport efficiency. Kunming's long-term goal is to shift the burden of public transit from buses to rail lines. Besides reporting on the implementation phase and impacts of Kunming's first center-lane bus lane, an overview of existing challenges and proposed improvements is presented.
The full length genome of waterfowl parvovirus were amplified using polymerase chain reaction(PCR) method and cloned.The DNA fragments were sequenced and compared with reference goose parvovirus strains and muscovy duck parvovirus strains.The results showed that the NS gene contained 1884 bp encoding 627 amino acids.The vp1 gene copntained 2199 bp encoding 732 amino acids.The NS gene between GPV and DPV showed 80.9 %-82.9 % and 89.5 %-91.2 % identities at nucleotide and amino acid levels,respectively.The vp1 genes showed 79.7 %-88.7 % and 85.5 %-93.3 % homologies at nucleotide and amino acid level,respectively.The sequence identities among GPV ranged from 88.8 %-99.6 %(nt) and 91.5 %-99.2 %(aa) and among DPV ranged from 98.1 % to 99.6 %(nt) and from 97.1 % to 98.8 %(aa),respectively.We speculate that a common ancestral parvovirus could have branched into DPV and GPV at late evolutionary stage in a host dependent manner.
ve To study the effect of operation on the patient pancreatic lithiasis. Methods Excide pancreatic duct, fetch net lithiasis, patchy jejunal loop was anastomosed to pancreatic duct in accordance with the peristalsis of jejunum. Results The operation relieved abdominal pain and impoved clinical sympton, but fatty diarrhea and diabetds could not be avoided. Conclusion The operation can disengage pancreatic duct obstruction, drain away the pancreatic fluid, prevent reflux and anastomotic leakage , is an effective treatment for pancreatic lithiasis.
Objective
To analyze the donor specific antibody (DSA) in liver transplantation, and discuss the therapeutic schemes.
Methods
We retrospectively analyzed prospectively collected samples from 139 cases of liver transplantation from September 1, 2013 to July 1, 2015.Luminex assays were applied to determine human leukocyte antigen, panel reactive antibody (PRA). For PRA positive cases, DSA, C1q and C4d were detected, and liver biopsy was done.
Results
Of 139 cases enrolled, there were 12 cases positive for DSAs, including 2 cases of PreDSA: 1 case of ⅠDSA (HLA-A mismatch), and 1 case of ⅡDSA (HLA-DQ mismatch). Ten cases of de novo DSA (including 1 case of PreDSA) all were HLA-DQ mismatch.The liver biopsy on 5 cases showed hepatic fibrosis, early rejection and intrahepatic cholestasis, and only 2 cases showed positive C4d.Of 6 cases of DSA, 5 cases showed positive C1q.In the patients positive for DSA, tacrolimus dose was adjusted postoperatively, adding mycophenolatemofetil or increasing its dose, or methylprednisolone and immunoglobulin given.
Conclusion
DSAs are important indicators of sensitized recipients in liver transplantation, associated with trends toward worse outcomes in patients or allografts.The monitoring of DSA is requisite in order to adjust the immunosuppressant.
Key words:
donor specific antibody; liver transplantation; immunoglobulin; complement C1q; complement C4d
Objective
To Analyze the clinical outcomes of pediatric liver transplantation (LT) for liver-based metabolic disorders.
Methods
We conducted a retrospective analysis on 42 pediatric patients with liver-based metabolic disorders from June 2013 to March 2017, and analyzed the pediatric end stage liver disease model (PELD), growth and development, type of transplant, postoperative complications and prognosis of patients.
Results
There were 42 children with liver-based metabolic disorders (15.56%) out of all the 270 children who underwent LT.The median age was 51.0 months (range, 3.4-160.9 months). Of the 42 children, 19 received living donor liver transplantation (LDLT), 18 cases received deceased donor liver transplantation (DDLT) and 5 cases received domino liver transplantation.1-, 2- and 3-year cumulative survival rate of 42 recipients was 97.7%, 93.6% and 93.6%, and that of the grafts was 95.3%, 91.4% and 91.4%, respectively.As compared with the 194 children with biliary atresia who underwent LT, significant difference was found in PELD and weight Z-score between the two groups.
Conclusion
Liver transplantation is a valuable option for children with metabolic disorders, and it has gained a better prognosis.
Key words:
Liver transplantation; Child; Metabolic disease
Objective To systematically evaluate the effects of physical exercise on the quality of life (QOL) of healthy older adults in China. Methods Relevant articles published until December 2021 were retrieved from China National Knowledge Infrastructure, Wanfang, China Science and Technology Journal Database, PubMed, EBSCO, Web of Science, and the Library of Congress. Inclusion criteria were studies in which the subjects were healthy Chinese older adults (aged ≥ 60 years), the reported sample size was clear, and the study design was a randomized controlled trial or a research study. In addition, studies were included if they reported the use of at least one QOL questionnaire and investigated at least one form of physical exercise. Results In total, 19 studies met the inclusion criteria, which included six studies that used comprehensive physical exercise type as an intervention and 13 studies that used regular physical exercise as an intervention. All 19 studies compared intervention and control groups, of which 12 (63%) were investigative studies and seven (37%) were experimental studies. Of the experimental studies, five used a positive control group and two used a negative control group. All 19 studies reported that physical exercise had varying degrees of positive effects on the QOL in older adults. Body–mind exercise was effective in improving the physical and mental health (MH) of older adults, whereas Xiyangcao only had a positive effect on physical health. Compared with no exercise or other exercise (exercise not used in the intervention group), the exercise group in the survey had a positive effect on the QOL of older adults. Regardless of the type of control group used, the exercise group in the experimental studies showed a positive effect of exercise on the QOL of older adults. Conclusion Physical exercise has a positive impact on the QOL of healthy older adults. However, due to the wide and varied scope of the included studies, more randomized controlled trials are needed to examine the effects of different types, intensities, durations, and the frequency of exercise on QOL. Systematic Review Registration [ https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=220115 ], identifier: [CRD42020220115].
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