Ahlem El Ferran

École Nationale Supérieure Agronomique

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Ali Atoui

Lebanese University

Hocine Boutoumi

University of Blida

Jean-Pierre Thermoz

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

Noureddine Bouras

École Normale Supérieure de Kouba

Hadjira Belkahla

University of Blida

Caroline Chatillon

Institut des Sciences des Plantes de Montpellier

Mahfoud Bakli

University of Ghardaia

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Laboratoire de Génie Chimique

Institut National Polytechnique de Toulouse

Université Toulouse III - Paul Sabatier

University of Blida

University of Ghardaia

Lebanese University

American University of Beirut Medical Center

American University of Beirut

National Council for Scientific Research

Lebanese Association for Energy Saving & for Environment

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Application of Real Time PCR for the Prevalence Determination of Citrus Exocortis Viroid (CEVd) in Algeria

Advanced Research in Life Sciences (2023)

Ahlem El Ferran Hadjira Belkahla Noureddine Bouras Ali Atoui Mahfoud Bakli

Abstract Citrus exocortis is a grafting disease caused by Citrus Exocortis Viroid (CEVd). The knowledge of the viroid’s incidence and distribution are necessary to further apply control measures. The objective of this work was to apply the real time PCR assay for the detection of CEVd in samples collected from symptomatic CEVd-infected plants in Mitidja (North Algeria). The assay showed an excellent diagnostic specificity where 38 out of 50 samples showed a positive reaction for CEVd, which revealed the presence of CEVd in citrus orchards with a prevalence of 76%. Consequently, this work offers a quick alternative to conventional methods for the early diagnosis and the prevalence assessment of CEVd.

10.2478/arls-2023-0008

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