H. Fiona Rodgers

College of St. Scholastica

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i-10 index

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Patrick J. Baker

University of Sheffield

David W. Rice

National University of Malaysia

K.L. Britton

University of Sheffield

T.C. Eadsforth

University of Dundee

T R Hawkes

Syngenta (United Kingdom)

Svetlana E. Sedelnikova

University of Sheffield

C. Bisson

University of Sheffield

Russell C. Viner

Syngenta (United Kingdom)

D.W. Rice

Stanford University

S.E. Sedelnikova

University of Sheffield

Cooperative Institutions

University of Sheffield

University of Malta

Royal College of Midwives

British Institute of Radiology

Newcastle University

University of Oxford

Syngenta (United Kingdom)

University of Wyoming

Wyoming Department of Education

Czech Academy of Sciences, Institute of Biotechnology

Author Statistics

Papers

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Sustaining Vulnerable Agroecosystems: Compost Provides Lasting Benefits to Soil Health and Carbon Storage in Semiarid Winter Wheat (Triticum Aestivum, L.)

H. Fiona Rodgers Jay B. Norton Urszula Norton Linda van Diepen

Agroecosystem

Soil carbon

10.2139/ssrn.4736346

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Crystal structure of IGPD from Pyrococcus furiosus in complex with (R)-C348

C. Bisson K.L. Britton S.E. Sedelnikova H. Fiona Rodgers T.C. Eadsforth

Pyrococcus furiosus

10.2210/pdb5dnl/pdb

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Crystallization and analysis of the subunit assembly and quaternary structure of imidazoleglycerol phosphate dehydratase fromSaccharomyces cerevisiae

Acta Crystallographica Section D Biological Crystallography (1995)

K. W. Wilkinson Patrick J. Baker David W. Rice H. Fiona Rodgers Timothy J. Stillman

Imidazoleglycerol phosphate dehydratase (IGPD) from Saccharomyces cerevisiae has been crystallized in the presence of a range of divalent cations using the hanging-drop method of vapour diffusion with ammonium sulfate or polyethylene glycol (PEG) 4000 as the precipitants. X-ray precession photographs have established that the crystals formed with ammonium sulfate (form A) belong to the space group F432, with cell parameter a = 177.5 A and a single subunit in the asymmetric unit. A preliminary data set collected to 6 A resolution on a two-detector San Diego Multiwire area detector has established that the crystals formed with PEG 4000 (form B) belong to either of the special pair of space groups I23 or I2(1)3, with cell parameter a = 131.0 A. A self-rotation function has been calculated using these data and indicates that the cell axes show pseudo fourfold symmetry consistent with a dimer in the asymmetric unit in this crystal form. Light-scattering studies indicate that in the presence of Mn(2+) and a number of other divalent cations IGPD undergoes assembly to a particle of molecular weight approximately 500 kDa. Given the subunit molecular weight of 23 kDa together with the symmetry of the crystals it would indicate that the most likely quaternary structure for this enzyme is based on a 24-mer in 432 symmetry.

Ammonium sulfate

Protein quaternary structure

Divalent

10.1107/s0907444995001569

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Citations (7)

The structure of wt A. thaliana IGPD2 in complex with Mn2+ and phosphate

C. Bisson K.L. Britton S.E. Sedelnikova H. Fiona Rodgers T.C. Eadsforth

10.2210/pdb4qnk/pdb

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Mirror‐Image Packing Provides a Molecular Basis for the Nanomolar Equipotency of Enantiomers of an Experimental Herbicide

Angewandte Chemie (2016)

C. Bisson K.L. Britton Svetlana E. Sedelnikova H. Fiona Rodgers T.C. Eadsforth

Abstract Programs of drug discovery generally exploit one enantiomer of a chiral compound for lead development following the principle that enantiomer recognition is central to biological specificity. However, chiral promiscuity has been identified for a number of enzyme families, which have shown that mirror‐image packing can enable opposite enantiomers to be accommodated in an enzyme's active site. Reported here is a series of crystallographic studies of complexes between an enzyme and a potent experimental herbicide whose chiral center forms an essential part of the inhibitor pharmacophore. Initial studies with a racemate at 1.85 Å resolution failed to identify the chirality of the bound inhibitor, however, by extending the resolution to 1.1 Å and by analyzing high‐resolution complexes with the enantiopure compounds, we determined that both enantiomers make equivalent pseudosymmetric interactions in the active site, thus mimicking an achiral reaction intermediate.

Enantiopure drug

Chirality

10.1002/ange.201607185

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Citations (2)

Case C-303/06Colemanv.Attridge Law and Steve LawJudgment of the ECJ 17 July 2008; Judgment of the Employment Tribunal, London, 30 September 2008

Journal of Social Welfare and Family Law (2009)

Tamara Hervey Maddy Reeves H. Fiona Rodgers Ben Riding Tom Roberts

Who should bear the economic (and very human) costs of caring for a disabled child? Is this something that should be shouldered by parents alone, even if this means they cannot effectively particip...

10.1080/09649060903354621

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Citations (2)

Crystal Structures Reveal that the Reaction Mechanism of Imidazoleglycerol-Phosphate Dehydratase Is Controlled by Switching Mn(II) Coordination

Structure (2015)

C. Bisson K.L. Britton Svetlana E. Sedelnikova H. Fiona Rodgers T.C. Eadsforth

Imidazoleglycerol-phosphate dehydratase (IGPD) catalyzes the Mn(II)-dependent dehydration of imidazoleglycerol phosphate (IGP) to 3-(1H-imidazol-4-yl)-2-oxopropyl dihydrogen phosphate during biosynthesis of histidine. As part of a program of herbicide design, we have determined a series of high-resolution crystal structures of an inactive mutant of IGPD2 from Arabidopsis thaliana in complex with IGP. The structures represent snapshots of the enzyme trapped at different stages of the catalytic cycle and show how substrate binding triggers a switch in the coordination state of an active site Mn(II) between six- and five-coordinate species. This switch is critical to prime the active site for catalysis, by facilitating the formation of a high-energy imidazolate intermediate. This work not only provides evidence for the molecular processes that dominate catalysis in IGPD, but also describes how the manipulation of metal coordination can be linked to discrete steps in catalysis, demonstrating one way that metalloenzymes exploit the unique properties of metal ions to diversify their chemistry.

Catalytic cycle

Coordination complex

Enzyme Catalysis

10.1016/j.str.2015.05.012

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Citations (33)

Crystallization and preliminary X-ray analysis ofD-2-hydroxyacid dehydrogenase fromHaloferax mediterranei

Acta Crystallographica Section F Structural Biology and Crystallization Communications (2009)

J. Domenech Patrick J. Baker S.E. Sedelnikova H. Fiona Rodgers David W. Rice

D-2-hydroxyacid dehydrogenase (D2-HDH) from Haloferax mediterranei has been overexpressed in Escherichia coli, solubilized in 8 M urea and refolded by rapid dilution. The protein was purified and crystallized by the hanging-drop vapour-diffusion method using ammonium sulfate or PEG 3350 as precipitant. Two crystal forms representing the free enzyme and the nonproductive ternary complex with alpha-ketohexanoic acid and NAD(+) grew under these conditions. Crystals of form I diffracted to beyond 3.0 A resolution and belonged to the monoclinic space group P2(1), with unit-cell parameters a = 66.0, b = 119.6, c = 86.2 A, beta = 96.3 degrees . Crystals of form II diffracted to beyond 2.0 A resolution and belonged to the triclinic space group P1, with unit-cell parameters a = 66.5, b = 75.2, c = 77.6 A, alpha = 109.1, beta = 107.5, gamma = 95.9 degrees. The calculated values for V(M) and analysis of the self-rotation and self-Patterson functions suggest that the asymmetric unit in both crystal forms contains two dimers related by pseudo-translational symmetry.

Triclinic crystal system

Monoclinic crystal system

Ammonium sulfate

Crystal (programming language)

10.1107/s174430910900863x

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Use of chemical modification in the crystallization of isocitrate lyase from Escherichia coli

Journal of Molecular Biology (1991)

Sarath I.B. Abeysinghe Patrick J. Baker David W. Rice H. Fiona Rodgers Timothy J. Stillman

Lyase

Crystal (programming language)

Protein crystallization

10.1016/0022-2836(91)90376-h

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Citations (10)

Effects of Dietary Pattern on Average Cortical Bone Density and Estimated Thickness of the Proximal Ulna

The FASEB Journal (2022)

Austin Armon Fayja Erceg Bailey Lochner H. Fiona Rodgers Kaya G Zelazny

There is an established relationship between diet and phenotype in various human body systems. Vegetarian diets may contain lower quantities of calcium and protein compared to other diets, causing speculation that this dietary pattern may contribute to lower bone mineral density (BMD) or osteoporosis. Nutritional intake is a major element in a person's overall Bone Mineral Density (BMD), but little is known about how the upper extremity responds to changes in dietary pattern. There is also conflicting evidence for the effects of a vegetarian diet on bone, with some studies suggesting that lower quantities of calcium and protein compared to other diets contributes to lower bone mineral density (BMD), while others find that the lower acidic load found in the vegetarian diet combined with a higher intake of potassium have a calcium-sparing effect with positive effects on bone health. This study tests how vegetarian and convenience diets affect Cortical Bone Thickness (CBT) and Average Cortical Density (ACD) of the olecranon process in humans, a site of fracture in the upper limb. Computed tomography (CT) scans with varying dietary pattern were obtained from the New Mexico Decedent Image Database and segmented in 3D Slicer. Cortical thickness was measured by calculating the difference between periosteal and endosteal section contours. Density was approximated by comparing the average radiodensity after applying a threshold of 500 HU to isolate cortical bone. MANOVA and post-hoc ANOVA testing were conducted in R Studio to test differences between dietary groups. There was a statistically significant effect of bone length (p = 0.001) and diet (p = 0.014) on both proximal ulnar bone properties measured (approximated cortical thickness and density). Post-hoc testing found that only bone length had a significant effect on cortical thickness (p = 0.003) while diet, but not bone length or sex, had a significant effect on average cortical density (p = 0.008). These results provide evidence of detrimental effects of a convenience diet on bone density, and possible evidence for beneficial effects of a vegetarian diet. Convenience foods are high in saturated fats, sodium, and refined sugars, which have been shown to have negative effects on bone mass and bone microarchitecture in rodents. Our results suggest that similar effects may be seen in the cortical bone of humans, providing additional evidence for the negative effects of convenience food on health. Previous studies have found that nutritional acidic load, which may be lowered by a vegetarian diet, is positively correlated with fracture risk and negatively correlated with BMD. Our results support these findings and add to the growing evidence for the benefits of a vegetarian diet.

Densitometry

10.1096/fasebj.2022.36.s1.0r600

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