Wim Jiskoot

Centre for Human Drug Research

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Daan J.A. Crommelin

Utrecht University

Gideon Kersten

Centre for Human Drug Research

Joke A. Bouwstra

Leiden University

Bram Slütter

Centre for Human Drug Research

Andrea Hawe

Bayer (Germany)

Wim E. Hennink

Utrecht University

Stefan Romeijn

Centre for Human Drug Research

Huub Schellekens

Erasmus MC

Koen van der Maaden

Leiden University

Alexander Kros

Leiden University

Cooperative Institutions

Leiden University

268

Utrecht University

182

Centre for Human Drug Research

147

Leiden University Medical Center

110

National Institute for Public Health and the Environment

University of Groningen

University of Amsterdam

Pharmo Institute

Radboud University Nijmegen

University Medical Center Utrecht

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Mass Spectrometric Analysis of Intact Human Monoclonal Antibody Aggregates Fractionated by Size-Exclusion Chromatography

Pharmaceutical Research (2010)

Başak Kűkrer Vasco Filipe Esther van Duijn Piotr T. Kasper Rob J. Vreeken

The aim of this study was to develop a method to characterize intact soluble monoclonal IgG1 antibody (IgG) oligomers by mass spectrometry.IgG aggregates (dimers, trimers, tetramers and high-molecular-weight oligomers) were created by subjecting an IgG formulation to several pH jumps. Protein oligomer fractions were isolated by high performance size exclusion chromatography (HP-SEC), dialyzed against ammonium acetate pH 6.0 (a mass spectrometry-compatible volatile buffer), and analyzed by native electrospray ionization time-of-flight mass spectrometry (ESI-TOF MS).Monomeric and aggregated IgG fractions in the stressed IgG formulation were successfully isolated by HP-SEC. ESI-TOF MS analysis enabled us to determine the molecular weight of the monomeric IgG as well as the aggregates, including dimers, trimers and tetramers. HP-SEC separation and sample preparation proved to be necessary for good quality signal in ESI-TOF MS. Both the HP-SEC protocol and the ESI-TOF mass spectrometric technique were shown to leave the IgG oligomers largely intact.ESI-TOF MS is a useful tool complementary to HP-SEC to identify and characterize small oligomeric protein aggregates.

Oligomer

Molecular mass

10.1007/s11095-010-0224-5

Cite

Citations (114)

Towards the development of a supercritical carbon dioxide spray process to coat solid protein particles

The Journal of Supercritical Fluids (2017)

Miao Yu Ahmad S. Sediq Shiduo Zhang M. Reza Nejadnik Hayley A. Every

Supercritical Carbon Dioxide

Process development

10.1016/j.supflu.2017.12.014

Cite

Citations (3)

Vaccines

Pharmaceutical biotechnology (2024)

Wim Jiskoot Gideon Kersten Enrico Mastrobattista Bram Slütter

10.1007/978-3-031-30023-3_15

Cite

Citations (0)

Stabilisation of therapeutic proteins by supercritical fluid drying technologies II

Andréanne Bouchard Gerard W. Hofland Wim Jiskoot

Source

Cite

Citations (0)

Micelle-Associated Protein in Epoetin Formulations: A Risk Factor for Immunogenicity?

Pharmaceutical Research (2003)

Suzanne Hermeling Huub Schellekens Daan J.A. Crommelin Wim Jiskoot

10.1023/b:pham.0000008034.61317.02

Cite

Citations (124)

Repeated fractional intradermal dosing of an inactivated polio vaccine by a single hollow microneedle leads to superior immune responses

Journal of Controlled Release (2016)

P. R. T. Schipper Koen van der Maaden Stefan Romeijn C.W.J. Oomens Gideon Kersten

Intradermal injection

CpG Oligodeoxynucleotide

Bolus (digestion)

10.1016/j.jconrel.2016.07.055

Cite

Citations (41)

Immunogenicity of meningococcal PorA formulations encapsulated in biodegradable microspheres

European Journal of Pharmaceutical Sciences (2003)

Carmen Arigita Joost van den Berg Karin Wensink Mies van Steenbergen Wim E. Hennink

Mannan

Calcein

10.1016/j.ejps.2003.09.006

Cite

Citations (11)

Cytosolic Delivery of Liposomally Targeted Proteins Induced by Photochemical Internalization

Pharmaceutical Research (2007)

Marjan M. Fretz Anders Høgset Gerben A. Koning Wim Jiskoot Gert Storm

The application of therapeutic proteins is often hampered by limited cell entrance and lysosomal degradation, as intracellular targets are not reached. By encapsulation of proteins into targeted liposomes, cellular uptake via endocytosis can be enhanced. To prevent subsequent lysosomal degradation and promote endosomal escape, photochemical internalization (PCI) was studied here as a tool to enhance endosomal escape. PCI makes use of photosensitising agents which localize in endocytic vesicles, inducing endosomal release upon light exposure.The cytotoxic protein saporin was encapsulated in different types of targeted liposomes. Human ovarian carcinoma cells were incubated with the photosensitiser TPPS2a and liposomes. To achieve photochemical internalization, the cells were illuminated for various time periods. Cell viability was used as read-out. Illumination time and amount of encapsulated proteins were varied to investigate the influence of these parameters.The cytotoxic effect of liposomally targeted saporin was enhanced by applying PCI, likely due to enhanced endosomal escape. The cytotoxic effect was dependent on the amount of encapsulated saporin and the illumination time.PCI is a promising technique for promoting cytosolic delivery of liposomally targeted saporin. PCI may also be applicable to other liposomally targeted therapeutic proteins with intracellular targets.

Saporin

Internalization

10.1007/s11095-007-9338-9

Cite

Citations (42)

C1 Vaccines

Birkhäuser Basel eBooks (2011)

Gideon Kersten Wim Jiskoot

Vaccines are the most commonly administered immunotherapeutics. Supported by great improvements in sanitation facilities such as safe drinking water, vaccination was the most effective measure to control a diversity of life-threatening infectious diseases in the 20th century. The most impressive success of vaccination was the global eradication of smallpox in the 1970s. Moreover, the incidence of many other infectious diseases, such as diphtheria, tetanus, pertussis, poliomyelitis, measles, mumps, and rubella, has been drastically reduced thanks to extensive vaccination programmes.

10.1007/978-3-0346-0136-8_16

Cite

Citations (0)

Effect of excipients on the encapsulation efficiency and release of human growth hormone from dextran microspheres

European Journal of Pharmaceutics and Biopharmaceutics (2007)

K.D.F. Vlugt-Wensink Y.J. Meijer M.J. van Steenbergen R. Verrijk Wim Jiskoot

Microparticle

Liberation

Polyvinylpyrrolidone

Excipient

10.1016/j.ejpb.2007.04.011

Cite

Citations (13)