The Group ACYW135 meningococcal polysaccharide vaccine (MPV-ACYW135) is a classical common vaccine used to prevent Neisseria meningitidis serogroups A, C, Y, and W135, but studies on the vaccine at the transcriptional level are still limited. In the present study, mRNAs and lncRNAs related to immunity were screened from the spleens of mice inoculated with MPV-ACYW135 and compared with the control group to identify differentially expressed mRNAs and lncRNAs in the immune response. The result revealed 34375 lncRNAs and 41321 mRNAs, including 405 differentially expressed (DE) lncRNAs and 52 DE mRNAs between the MPV group and the control group. Results of GO and KEGG enrichment analysis turned out that the main pathways related to the immunity of target genes of those DE mRNAs and DE lncRNAs were largely associated with positive regulation of T cell activation, CD8-positive immunoglobulin production in mucosal tissue, alpha-beta T cell proliferation, negative regulation of CD4-positive, and negative regulation of interleukin-17 production, suggesting that the antigens of MPV-ACYW135 capsular polysaccharide might activate T cell related immune reaction in the vaccine inoculation. In addition, it was noted that Bach2 (BTB and CNC homolog 2), the target gene of lncRNA MSTRG.17645, was involved in the regulation of immune response in MPV-ACYW135 vaccination. This study provided a preliminary catalog of both mRNAs and lncRNAs associated with the proliferation and differentiation of body immune cells, which was worthy of further research to enhance the understanding of the biological immune process regulated by MPV-ACYW135.
Abstract Background : We have designed a modified sinus tarsi approach (MSTA) to treat sanders type Ⅱ or Ⅲ intra-articular calcaneal fractures, providing sufficient surgical field exposure and operation space, and significantly reducing the rate of wound complications. We compared the radiologic results, clinical outcomes, and complications of patients operated on via the MSTA and the extended lateral approach (ELA) for treatment of displaced intraarticular calcaneal fractures Sanders type Ⅱ or Ⅲ. Methods : We retrospectively studied the utility of the ELA (32 patients, 34 feet) and the MSTA (33 patients, 36 feet) operated on from January 2013 to January 2018. The average follow-up time of the two groups was 58 months. We assessed the preoperative and final x-rays, clinical outcomes and complications at the last follow-up. Clinical outcomes were evaluated using the American Orthopaedic Foot and Ankle Society (AOFAS) score and visual analog scale (VAS). Results : In both groups, the average final width, height, Bohler angle were significantly restored compared with the preoperative width, height, and Bohler. The last follow-up calcaneal length, width, height, Bohler angle, Gissane angle did not differ significantly between the 2 groups. In terms of the clinical outcomes, the average AOFAS score of the ELA group checked to 82.1 points, yielding a 77.8% excellent or good rating, and the average VAS score was 1.76 points. In contrast, the average AOFAS score of the MSTA group checked to 81.0 points, yielding a 79.4% excellent or good rating, and the average VAS score was 1.68 points. There was no significant difference in AOFAS score and VAS score between the 2 groups. The wound-healing complication rate was 2.9% in MSTA group and 22.2% in ELA group (p = .040). 4 cases of sural nerve injury occurred in ELA group, and 2 in MSTA group. 1 cases of peroneal tendon complication only occurred in ELA group. Conclusion : Compared with ELA, MSTA provides similar and favorable radiological and clinical results, and is associated with fewer wound complications. We believe the MSTA is more appropriate in the treatment of Sanders II and III calcaneal intra-articular fractures.
• Liver transplantation is a major life-saving procedure and donation after cardiac death (DCD) has increased the pool of potential liver donors. • However, livers procured after DCD are at increased risk of primary graft dysfunction and biliary tract ischaemia. Normothermic extracorporeal liver perfusion (NELP) may increase the ability to protect, evaluate and, in future, transplant DCD livers. • We conducted a proof-of-concept experiment using a human liver procured by DCD (deemed not suitable for liver donation) to assess the short-term (3 hours) feasibility, histological effects and functional efficacy of NELP. • We used an extracorporeal membrane oxygenation circuit with separate hepatic artery and portal vein perfusion to achieve physiological perfusion pressures, and coupled this with parenteral nutrition and an insulin infusion. We achieved NELP with evidence of liver function (bile production, paracetamol removal and control of ammonia, bilirubin and lactate levels) for 3 hours. There was essentially normal liver and biliary tract histology after 8 hours of perfusion. • Our experiment justifies further investigation of the feasibility and efficacy of human DCD liver preservation by NELP.
The title compound, C(23)H(22)O(8), is a binary benzoyl ester whose nucleus consists of a fused system made up of a methyl-enedi-oxy ring and two tetra-hydro-furan rings. One of the benzoyl ester groups is attached at the junction of the two tetra-hydro-furan rings. The other is attached to the outer tetra-furan ring. Both the benzoyl ester groups are in an axial conformation with respect to the outer tetrhydro-furan ring. In the crystal, mol-ecules are linked by two weak C-H⋯O hydrogen bonds, forming a chain running parallel to the a axis.
Hepatocellular carcinoma (HCC) is a common malignant tumor lacking sensitive biomarkers for prognosis. Sox3, a member of the Sex determining region Y box gene superfamily, has been demonstrated to be an oncogene in many cancers. However, the expression and clinical importance of Sox3 in HCC remains elusive. In this study, fifty pairs of HCC tissues with adjacent non-tumor samples were collected for detecting Sox3 expression by qPCR and immunoblotting analyses. A total of 104 HCC tissues were included for immunohistochemistry assay and analyzed by immunostaining scores. The correlation of Sox3 expression with clinicopathological factors and prognosis of HCC patients were calculated. Sox3 expression in HCC tissues was significantly higher than that in the non-tumor counterparts at the mRNA and protein levels. High staining scores of Sox3 was detected in 75.96% of HCC tissues. Statistical analyses demonstrated that highly expressed Sox3 was significantly correlated with low tumor capsule formation, advanced tumor stage and poor tumor differentiation. Moreover, patients with high Sox3 expression showed worse recurrence-free survival and overall survival than those with low Sox3 expression, and multivariate analyses further indicated that status of Sox3 expression is an independent prognostic factor in HCC patients. Therefore, our results suggested that overexpression of Sox3 in HCC tissues is correlated with increased tumor development and poor prognosis in HCC.
Abstract Background The treatment of calcaneal fractures is not uniform. This study aimed to compare the functional and imaging results of subtalar arthroscopic reduction combined with hollow screw fixation (SJAHF) and the extended lateral approach (ELA) for the treatment of Sanders type II and III displaced intra-articular calcaneal fractures (DIACFs). Methods From January 2020 to January 2023, 60 patients with calcaneal fractures were treated with SJAHF or ELA for foot and ankle surgery at the Second Affiliated Hospital of Anhui Medical University. Changes in calcaneal Böhler’s angle, the Gissane angle, length, height, and width were recorded before, after, and at the 1-, 3-, 6-, 12-month, and last follow-up. The preoperative waiting time, operation time, hospital stay, and other data of each patient were analyzed. The visual analogue scale (VAS) and American Orthopaedic Foot and Ankle Society (AOFAS) scores were used to evaluate clinical effects. Results All 60 patients were followed up for at least 12 months. There was no statistical difference in baseline data between groups (P > 0.05). The SJAHF group was superior to the ELA group regarding the preoperative waiting time, hospital stay, and intraoperative fluoroscopy times; however, the operative time of the SJAHF group was greater (P < 0.05). There were no significant differences in Böhler’s angle, the Gissane angle, length, height, or width between the two groups at any time point (P > 0.05). These imaging values were significantly improved after surgery and at the last follow-up (P < 0.05). The VAS scores of the patients in the SJAHF group at the last follow-up were significantly different from those in the ELA group (P < 0.05). The final AOFAS score and incidence of postoperative complications in the SJAHF group were better; however, the difference was not statistically significant (P > 0.05). Simultaneously, total weight-bearing and time to return to work in the SJAHF group were earlier (P < 0.05). Conclusion Both SJAHF and ELA improved the clinical outcomes of patients with DIACFs. SJAHF reduces surgical wounds and maintains effective reduction and strong internal fixation. It has the advantages of a reduced preoperative waiting time, shortened hospital stay, reduced intraoperative fluoroscopy time, alleviated postoperative pain, and accelerated patient recovery.
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