Zhong-wei Shao

Lanzhou Veterinary Research Institute

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Xiaola Guo

Chinese Academy of Agricultural Sciences

Yadong Zheng

Lanzhou University

Juntao Ding

Xinjiang University

Xuenong Luo

Lanzhou Veterinary Research Institute

Xiao-lin Sun

Haitao Xiang

Suzhou Kowloon Hospital

Yujuan Shen

National Institute for Parasitic Diseases

Xueyong Zhang

Qinghai University

Jing Yang

Chinese Academy of Sciences

Jianping Cao

National Institute for Parasitic Diseases

Cooperative Institutions

Lanzhou Veterinary Research Institute

Chinese Academy of Sciences

Chinese Academy of Agricultural Sciences

Shanghai Jiao Tong University

Guangzhou University of Chinese Medicine

Nanchang University

National Institute for Parasitic Diseases

National Health and Family Planning Commission

Vector (United States)

Hospital for Tropical Diseases

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[Screening and Applications of qPCR Primers for apomucin Gene of Echinococcus multilocularis].

PubMed (2016)

Meng Su Xiaola Guo Jing Yang Zhong-wei Shao Juntao Ding

To screen for the optimal qPCR primers for Echinococcus multilocularis apomucin gene (Em-apo) and analyze Em-apo expression.Primers were designed based on 4 Em-apo sequences from GeneDB. Primer specificity and PCR efficiency were determined, based on which the optimal primer pairs were selected. Alterations of Em-apo expression in 1 000 E. multilocularis protoscoleces treated with albendazole（5 μg/ml） and insulin（100 ng/ml） were separately assessed using the selected primers. DMSO used in albendazole dilution and in PBS insulin dilution were used as the control.Specific primers for Em-apo-1, Em-apo-2/3, Em-apo-4 and actin were selected. qPCR melting curves revealed a single peak for each primer pair and an amplification efficiency from 95% to 101%. The qPCR showed increased expression of Em-apo-1（1.51±0.27）, Em-apo-2/3 （1.39±0.30） and Em-apo-4（1.14±0.18） after albendazole treatment in comparison to the DMSO control（1.00）（P>0.05 among the three genes）; and an unaltered Em-apo-1 expression, slightly decreased Em-apo-4 expression, and significantly decreased Em-apo-2/3 expression（0.73±0.09） after insulin treatment in comparison to the PBS control (P>0.05 among the three genes）.The selected specific primers for Em-apo genes can be used to analyze the gene expression by qPCR. Treatment with albendazole and insulin show certain effects on the expression of Em-apo genes in E. multilocularis protoscoleces.

Echinococcus multilocularis

Primer (cosmetics)

Albendazole

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A survey on parasites in wild rodents in Xiji County, a northwestern part of China.

Wenfeng He Li Ke Xiaola Guo Y Chen Zhong-wei Shao

Rodents act as an indicator for evaluation of environment contaminations and public health risks caused by parasites. A survey of parasites in wild rodents was conducted in 14 villages in Xijin County, where alveolar echinococcosis by Echinococcus multilocularis is epidemic. In total, 72 rodents including 25 mice, 16 Citellus dauricus (Daurian ground squirrel), 12 squirrels and 19 mole rat (Myospalax fontanieri) were captured. Infections (2.8%) of Taenia taeniaeformis, which is transmitted mainly between mice and cats, were found in mice in Wangping (WP) and Miaoping (MP) villages, but other cestodes' infections were not observed. WP and MP isolates were principally similar in morphology but, unlike WP isolate, MP isolate had no hooks on the scolex. Using 18S rRNA as a biomarker, the phylogenetic analysis showed that WP and MP isolates grouped together with European and Asian isolates and formed a separate cluster. These results highlights the prevalence of T. taeniaeformis in cats or/and dogs and a risk of opportunistic infections in human populations.

Echinococcus multilocularis

Alveolar echinococcosis

Taenia

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Effects of Echinococcus multilocularis miR-71 mimics on murine macrophage RAW264.7 cells

International Immunopharmacology (2016)

Yadong Zheng Xiaola Guo Wei He Zhong-wei Shao Xueyong Zhang

Echinococcus multilocularis

Macrophage polarization

10.1016/j.intimp.2016.03.015

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Citations (51)