Andrew D. Frugé

Auburn University

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Wendy Demark‐Wahnefried

University of Alabama at Birmingham

Michael D. Roberts

SUNY Downstate Health Sciences University

Kristen S. Smith

Moffitt Cancer Center

Bradley A. Ruple

Auburn University

Joshua S. Godwin

Auburn University

Julie L. Locher

University of Alabama at Birmingham

Laura Q. Rogers

University of Alabama at Birmingham

Kaelin C. Young

Auburn University

Anna E. Arthur

University of Illinois Urbana-Champaign

Shelby C. Osburn

Auburn University

Cooperative Institutions

Auburn University

119

University of Alabama at Birmingham

University of Alabama

Washburn University

Applied Sciences Laboratory (United States)

University of Michigan–Ann Arbor

O'Neal Comprehensive Cancer Center

University of Illinois Urbana-Champaign

Mississippi State University

Harvard University

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Resistance Exercise and Mechanical Overload Upregulate Vimentin for Skeletal Muscle Remodeling

bioRxiv (Cold Spring Harbor Laboratory) (2024)

Joshua S. Godwin J. Max Michel Cleiton Augusto Libardi Andreas N. Kavazis Christopher S. Fry

ABSTRACT Our laboratory has performed various experiments examining the proteomic alterations that occur with mechanical overload (MOV)-induced skeletal muscle hypertrophy. In the current study we first sought to determine how 10 weeks of resistance training in 15 college-aged females affected protein concentrations in different tissue fractions. Training, which promoted significantly lower body muscle- and fiber-level hypertrophy, notably increased sarcolemmal/membrane protein content (+10.1%, p<0.05). Sarcolemmal/membrane protein isolates were queried using mass spectrometry-based proteomics, ∼10% (38/387) of proteins associated with the sarcolemma were up-regulated (>1.5-fold, p<0.05), and one of these targets (the intermediate filament vimentin; VIM) warranted further mechanistic investigation. VIM expression was first examined in the plantaris muscles of 4-month-old C57BL/6J mice following 10- and 20-days of MOV via synergist ablation. Relative to Sham (control) mice, VIM mRNA and protein content was significantly higher in MOV mice and immunohistochemistry indicated that VIM was predominantly present in the extracellular matrix (ECM). The 10- and 20-day MOV experiments were replicated in Pax7-DTA (tamoxifen-induced, satellite cell depleted) mice, which reduced the presence of VIM in the ECM. Finally, a third set of 10- and 20-day MOV experiments were performed in C57BL/6 mice intramuscularly injected with either AAV9-scrambled (control) or AAV9-VIM shRNA. While VIM shRNA mice presented with lower VIM in the ECM (∼50%), plantaris masses in response to MOV were similar between the injection groups. However, VIM shRNA mice presented with appreciably more MyHC emb -positive fibers with centrally located nuclei, indicating a regenerative phenotype. Using an integrative approach, we propose that skeletal muscle VIM is a mechanosensitive target predominantly localized to the ECM, and satellite cells are involved in its expression. Moreover, a disruption in VIM expression during MOV leads to dysfunctional skeletal muscle hypertrophy.

10.1101/2024.06.03.597241

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Acute and chronic effects of resistance training on skeletal muscle markers of mitochondrial remodeling in older adults

Physiological Reports (2020)

Paulo H. C. Mesquita Donald A. Lamb Hailey A. Parry Johnathon H. Moore Morgan A. Smith

We investigated the acute and chronic effects of resistance training (RT) on skeletal muscle markers of mitochondrial content and remodeling in older, untrained adults. Sixteen participants (n = 6 males, n = 10 females; age = 59 ± 4 years) completed 10 weeks of full-body RT (2 day/week). Muscle biopsies from the vastus lateralis were obtained prior to RT (Pre), 24 hr following the first training session (Acute), and 72 hr following the last training session (Chronic). Protein levels of mitochondrial electron transport chain complexes I–V (+39 to +180%, p ≤ .020) and markers of mitochondrial fusion Mfn1 (+90%, p = .003), Mfn2 (+110%, p < .001), and Opa1 (+261%, p = .004) increased following chronic RT. Drp1 protein levels also increased (+134%, p = .038), while Fis1 protein levels did not significantly change (−5%, p = .584) following chronic RT. Interestingly, protein markers of mitochondrial biogenesis (i.e., PGC-1α, TFAM, and NRF1) or mitophagy (i.e., Pink1 and Parkin) were not significantly altered (p > .050) after 10 weeks of RT. In summary, chronic RT promoted increases in content of electron transport chain proteins (i.e., increased protein levels of all five OXPHOS complexes) and increase in the levels of proteins related to mitochondrial dynamics (i.e., increase in fusion protein markers) in skeletal muscle of older adults. These results suggest that chronic RT could be a useful strategy to increase mitochondrial protein content in older individuals.

MFN2

FIS1

MFN1

PINK1

TFAM

NRF1

DNM1L

10.14814/phy2.14526

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Citations (46)

Symptom Management and Quality of Life in US Adults With Irritable Bowel Syndrome by Subtype

Current Developments in Nutrition (2024)

Sarah Lennon Dylan Travis J Greene Andrew D. Frugé

10.1016/j.cdnut.2024.102207

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Assessing the nutrition knowledge, beliefs, and behaviors of food pantry managers: implications for healthier food environments

Frontiers in Public Health (2025)

Sofia O. Sanchez Katie Funderburk Erin Reznicek Robert Bubb Andrew D. Frugé

Introduction Food pantry managers play a key role in determining the food environment of the pantry, which can influence their clients’ dietary composition. However, their impact on pantry food environments remains understudied. We sought to understand food pantry managers’ nutrition knowledge, beliefs, and behaviors (KBBs) in this study. Methods We surveyed 47 Alabama food pantry managers’ nutrition KBBs from November 2022 to November 2023. Validated and previously published tools used include the Consumer Nutrition Knowledge Scale, a diet beliefs scale, and a dietary-related consumer behavior questionnaire. Results The majority of managers were college-educated (54%), white (74%), and female (74%), with a mean age of 60 ± 13 years and an average of 7 ± 5.5 years of experience in managing food pantries. Managers reported positive nutrition beliefs and behaviors but scored lower on objective nutrition knowledge. Exploratory analyses indicated moderate to very strong associations between KBBs, pantry characteristics, and neighborhood characteristics. Conclusion Nutrition education for pantry managers, along with improved pantry infrastructural support, could play a key role in improving the healthfulness of the food provided.

10.3389/fpubh.2025.1544413

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Additional file 9 of Epigenetic stratification of head and neck cancer survivors reveals differences in lycopene levels, alcohol consumption, and methylation of immune regulatory genes

Figshare (2020)

Laura Moody Sylvia L. Crowder Andrew D. Frugé Julie L. Locher Wendy Demark‐Wahnefried

Additional file 9. Gene ontology enrichment analysis in the yellow group.

Risk Stratification

10.6084/m9.figshare.12948067

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Characterizing Demographic and Geographical Differences in Health Beliefs and Dietary Habits Related to Colon Cancer Risk in US Adults

Frontiers in Nutrition (2020)

Megan N. Schaberg Kristen S. Smith Michael W. Greene Andrew D. Frugé

Background: Colon cancer (CC) risk is increased by behavioral factors including a diet high in red meat (RM) and processed meat; excess adiposity has contributed to a rise in CC in younger adults. The willingness of at-risk adults to modify behaviors to reduce CC risk warrants further investigation. Methods: The previously validated Dietary Habits and Colon Cancer Beliefs Survey (DHCCBS) was used to assess attitudes and beliefs related to CC risk and diet behavior. An abbreviated food frequency questionnaire was included in the survey to quantify RM and green leafy vegetable (GLV) intake over the previous 30 days. Independent samples t-tests compared RM and GLV intake and DHCCBS responses. One-way analysis of variance with post-hoc LSD correction was completed to assess these differences within three age groups (<35, 35-44, and 45-54 years old) and between U.S. Census Bureau geographical regions. Results: 838 survey responses were analyzed. Perceived severity of CC diagnosis was significantly lower in younger adults (<35) compared to older adults (35-44, p=0.042; 45-54, p=0.003). Furthermore, younger adults (<35) perceived fewer barriers (i.e. taste preference) to GLV consumption than their older adult counterparts (35-44, p=0.019; 45-54, p=0.002). Few regional differences in habitual RM consumption were observed, however, several disparities were observed with GLV. Conclusion: These findings from the DHCCBS indicate health beliefs towards CC risk are influenced by an individual’s age and dietary habits. Additionally, regional differences in GLV consumption indicate opportunities for risk-reduction-focused health messages, particularly in the southern United States where CC incidence and mortality are highest.

Consumption

10.3389/fnut.2020.568643

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Citations (3)

Head and neck cancer survivors’ preferences for and evaluations of a post-treatment dietary intervention

Nutrition Journal (2019)

Sylvia L. Crowder Katherine G. Douglas Andrew D. Frugé William R. Carroll Sharon A. Spencer

Abstract Background Dietary preferences vary depending on cancer type. The purpose of this study was to report dietary intervention preferences and a study program evaluation from post-treatment head and neck cancer survivors participating in a dietary intervention. Methods Between January 2015 and August 2016, 24 head and neck cancer survivors participated in a 12-week randomized clinical dietary intervention trial that promoted weekly consumption of 2.5 cups of cruciferous vegetables and 3.5 cups of green leafy vegetables. At study completion, survivors completed a preferences survey and a study program evaluation to probe interests and improvement aspects for planning future dietary intervention trials. Descriptive statistics (means and frequencies) were generated for multiple choice question responses. Responses to open-ended questions were recorded and grouped based on themes, and verified by quality assurance checks by a second study team member. Results Twenty-three survivors completed the preferences and evaluation surveys (response rate 96%). Overall, most participants reported a preference for one-on-one telephone counseling from a registered dietitian nutritionist before beginning treatment. Ninety-six percent of participants ranked the overall study program as “very good” to “excellent,” and all agreed the objectives of the study were clear, the study staff was helpful and easy to contact, and the registered dietitian nutritionist was knowledgeable. Conclusions Future research and dietary intervention planning for head and neck cancer survivors should focus on strategies to promote one-on-one telephone or other distance-based counseling combined with face-to-face visits, according to survivor preference.

Nutritionist

Clinical nutrition

10.1186/s12937-019-0479-6

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Citations (11)

Fecal Akkermansia muciniphila Is Associated with Body Composition and Microbiota Diversity in Overweight and Obese Women with Breast Cancer Participating in a Presurgical Weight Loss Trial

Journal of the Academy of Nutrition and Dietetics (2018)

Andrew D. Frugé William Van Der Pol Laura Q. Rogers Casey D. Morrow Yuko Tsuruta

Akkermansia muciniphila

Akkermansia

10.1016/j.jand.2018.08.164

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Citations (70)

Nutrition Knowledge of Collegiate Athletes in the United States and the Impact of Sports Dietitians on Related Outcomes: A Narrative Review

Nutrients (2021)

Aaron J. Riviere Rae Leach Haleigh Mann SAMUEL ROBINSON Donna Burnett

In the last decade, the number of full-time registered dietitians (RDs) serving intercollegiate athletes in the United States has more than quadrupled. However, many student athletes may be at increased risk of nutrition-related problems that impact physical and academic performance, which include inadequate macronutrients, inadequate micronutrients, and excessive macronutrients. This narrative review reports the current literature to date on nutrition-related knowledge in collegiate athletes and the impact of sports RDs on student athletes’ nutrition knowledge and behaviors. To date, only observational and quasi-experimental studies have been published with regard to changes in nutrition knowledge and behaviors in NCAA athletes. While these studies report benefits of the RD as a member of the interdisciplinary student athlete support team, more well-designed randomized control trials are warranted to determine benefits related to health outcomes and sport-specific performance outcomes.

Narrative review

Sports nutrition

10.3390/nu13061772

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Citations (27)

Resistance training increases muscle NAD+ and NADH concentrations as well as NAMPT protein levels and global sirtuin activity in middle-aged, overweight, untrained individuals

Aging (2020)

Donald A. Lamb Johnathon H. Moore Paulo H. C. Mesquita Morgan A. Smith Christopher G. Vann

We examined if resistance training affected muscle NAD+ and NADH concentrations as well as nicotinamide phosphoribosyltransferase (NAMPT) protein levels and sirtuin (SIRT) activity markers in middle-aged, untrained (MA) individuals. MA participants (59±4 years old; n=16) completed 10 weeks of full-body resistance training (2 d/wk). Body composition, knee extensor strength, and vastus lateralis muscle biopsies were obtained prior to training (Pre) and 72 hours following the last training bout (Post). Data from trained college-aged men (22±3 years old, training age: 6±2 years old; n=15) were also obtained for comparative purposes. Muscle NAD+ (+127%, p<0.001), NADH (+99%, p=0.002), global SIRT activity (+13%, p=0.036), and NAMPT protein (+15%, p=0.014) increased from Pre to Post in MA participants. Additionally, Pre muscle NAD+ and NADH in MA participants were lower than college-aged participants (p<0.05), whereas Post values were similar between cohorts (p>0.10). Interestingly, muscle citrate synthase activity levels (i.e., mitochondrial density) increased in MA participants from Pre to Post (+183%, p<0.001), and this increase was significantly associated with increases in muscle NAD+ (r2=0.592, p=0.001). In summary, muscle NAD+, NADH, and global SIRT activity are positively affected by resistance training in middle-aged, untrained individuals. Whether these adaptations facilitated mitochondrial biogenesis remains to be determined.

Sirtuin 1

10.18632/aging.103218

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Citations (48)