Colm A. Moran

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Broiler Tissue Enrichment with Docosahexaenoic Acid (DHA) through Dietary Supplementation with <i>Aurantiochytrium limacinum</i> Algae

Food and Nutrition Sciences (2018)

Colm A. Moran Jason D. Keegan Kirsi Vienola J. Apajalahti

The omega-3 fatty acid (n-3 FA) content of broiler tissues can be increased through dietary supplementation of hens with n-3 FA-rich microalgae. The aim of this study was to evaluate the effect of three different dietary inclusion levels of a docosahexaenoic acid (DHA)-rich microalgae (AURA) on broiler performance and the enrichment of tissues with n-3 FA. The randomized study was conducted using 352 birds, housed in 32 pens with 11 birds per pen. Pens were randomly assigned to one of four treatments, with each treatment replicated 8 times. The treatments included one unsupplemented control (0%) and three wheat-soya based experimental diets supplemented with AURA at a level of 0.5%, 1.5% and 2.5% for the starter, grower and finisher periods. Birds were weighed on days 0, 10, 24, 35 and 41, and feed intake was recorded per pen. On day 41, five birds per treatment were euthanized and individually weighed. Thigh muscle, breast muscle, liver, kidney and skin samples were taken post-mortem, freeze dried and DHA content quantified, following fat extraction and methylation, by GC-FID (AOAC 996.06 method). Performance and tissue data were analyzed by ANOVA with Dunnett's (2-sided) post-hoc test to determine the differences between the mean values for each treatment. Dietary supplementation with AURA had no effect on body weight or feed intake during any period of the study. For thigh muscle, kidney and skin the DHA increased linearly (P < 0.05) with increasing level of dietary AURA, whilst there was a quadratic response in uptake of DHA in breast muscle and liver. The study demonstrated the potential of efficiently enriching broiler meat and organs with DHA by feeding AURA.

Completely randomized design

10.4236/fns.2018.910084

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Citations (7)

Heterotrophic Aurantiochytrium sp. supplementation to layer diets sustainably increases the omega-3 concentration of eggs

British Poultry Science (2019)

Jason D. Keegan Dorothy Currie A. Knox Colm A. Moran

1. The consumption of adequate amounts of the long-chain polyunsaturated omega-3 fatty acids (n-3 LC-PUFA) has been associated with beneficial effects on human health. Eggs are commonly consumed worldwide, and their omega-3 content can be easily altered by changing the diets of laying hens and so represent an important target for enrichment.2. In this study, the effect of supplementing laying hens with DHA-rich, Aurantiochytrium limacinum at three different inclusion levels was investigated over a 24-week period.3. Significant increases in egg DHA concentrations were observed after four weeks and were maintained for the duration of the 24-week study. The supplemented eggs in the current study had a DHA content of 82, 101, and 129 mg/yolk when supplemented with 0.25%, 0.5% and 1% treatments, respectively, which meets the EU criteria to be considered 'high in omega-3ʹ.4. Using the sustainably grown protist Aurantiochytrium limacinum to supplement layer diets increased the egg DHA concentration and decreased the n-6/n-3 ratio, improving the nutritional value of the eggs for human consumers.

Yolk

10.1080/00071668.2019.1622079

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Citations (8)

Design and validation of a real-time PCR technique for assessing the level of inclusion of fungus- and yeast-based additives in feeds

Journal of Microbiological Methods (2020)

Teemu Rinttilä Kir Ülle J. Apajalahti Rebecca Timmons Colm A. Moran

A reliable method for quantification of non-viable microbe-based nutritional and zootechnical additives introduced into feed is essential in order to ensure regulatory compliance, feed safety and product authenticity in industrial applications. In the present work, we developed a novel real-time quantitative polymerase chain reaction (qPCR) -based analysis protocol for monitoring two microbial additives in feed matrices. To evaluate the applicability of the method, pelleted wheat- and maize-based broiler chicken diets containing a non-viable phytase-producing strain of Aspergillus niger produced in solid state fermentation (150 or 300 g/t) and a non-viable selenium-enriched Saccharomyces cerevisiae (100 or 200 g/t) as model feed ingredients, were manufactured and subjected to analysis. Power analysis of the qPCR results indicated that 2 to 6 replicate feed samples were required to distinguish the product doses applied, which confirms that the microbial DNA was efficiently recovered and that potential PCR inhibitors present in the feed material were successfully removed in DNA extraction. The analysis concept described here was shown to be an accurate and sensitive tool for monitoring the inclusion levels of non-viable, unculturable microbial supplements in animal diets.

Animal Feed

Phytase

Feed additive

Aspergillus niger

10.1016/j.mimet.2020.105867

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Citations (2)

Antimicrobial Susceptibility Data for Six Lactic Acid Bacteria Tested against Fifteen Antimicrobials

Data (2022)

Ivana Nikodinoska Jouni Heikkinen Colm A. Moran

Antimicrobial resistance is a rising threat in the agrifood sector. The misuse of antibiotics exerts selective pressure, driving resistance mechanisms in bacteria, which could ultimately spread through many routes and render treatments for infectious diseases inefficient in humans and animals. Herein, we report antimicrobial susceptibility data obtained for six lactic acid bacteria, the members of which are commonly used in the food and feed chain. Fifteen antimicrobials were considered for the phenotypic testing: ampicillin, gentamicin, kanamycin, tetracycline, erythromycin, clindamycin, chloramphenicol, streptomycin, vancomycin, quinupristin-dalfopristin, bacitracin, sulfamethoxazole, ciprofloxacin, linezolid, and rifampicin. The reported dataset could be used for the comparison, generation, and reconsideration of new and/or existing cut-off values when considering lactic acid bacteria, particularly lactobacilli and pediococci.

Linezolid

Enterococcus faecium

Fusidic acid

Quinupristin

10.3390/data8010010

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Citations (3)

Yeast polysaccharides in health promotion and disease alleviation.

Colm A. Moran Djordje B. Zeković Stefan Kwiatkowski T. P. Lyons K. A. Jacques

Source

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Citations (0)

The validation & verification of an LC/MS method for the determination of total docosahexaenoic acid concentrations in canine blood serum

Regulatory Toxicology and Pharmacology (2018)

Gerald Patrick Dillon Jason D. Keegan G.K. Wallace Alexandros Yiannikouris Colm A. Moran

10.1016/j.yrtph.2018.03.021

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Citations (5)

Draft Genome Sequence of Lacticaseibacillus rhamnosus IMI 507023

Microbiology Resource Announcements (2022)

Ivana Nikodinoska Jenny Makkonen Daniel Blande Colm A. Moran

We report here the draft genome sequence of Lacticaseibacillus rhamnosus strain IMI 507023, a lactic acid bacterium, isolated from corn silage in Nicholasville, Kentucky, USA. The strain is deposited in the Centre for Agriculture and Bioscience International (CABI) Culture Collection with the accession number IMI 507023.

Strain (injury)

Silage

Sequence (biology)

10.1128/mra.01217-21

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Citations (2)

Tolerance of Broilers to Dietary Supplementation with High Levels of the DHA-Rich Microalga, Aurantiochytrium Limacinum: Effects on Health and Productivity

Animals (2018)

Colm A. Moran Douglas A. Currie Jason D. Keegan A. Knox

It is well established that the docosahexaenoic acid (DHA) content of commonly consumed meats, such as chicken, can be increased through dietary supplementation with DHA-rich ingredients. The purpose of this study was to investigate the tolerance of broilers to dietary supplementation with the unextracted biomass of a DHA-rich microalgae Aurantiochytrium limacinum, so as to ensure its safety, since it is accumulated in broiler meat. Healthy day-old male Ross 308 chicks (n = 1120) were evenly distributed to 32 pens (35 chicks per pen), with pens randomly allocated to one of four dietary treatments, each having eight replicates. The dietary groups included one untreated control and three treatments corresponding to three inclusion levels (0.5, 2.5, and 5.0%) of All-G-Rich®, with the birds receiving the experimental diets ad libitum during the study (day 0–42). Bird survival, blood parameters, productivity, and breast and thigh DHA content were determined after 42 days of feeding. Supplementation at up to 10 times the intended use level had no negative effects on the mortality, blood parameters or productivity of the birds, while significant increases in the meat DHA content were observed. These results indicate that supplementation with Aurantiochytrium limacinum is a safe and effective way to increase broiler tissue DHA content.

10.3390/ani8100180

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Citations (24)

Toxicological evaluation of an unextracted Aurantiochytrium limacinum biomass, a novel docosahexaenoic acid rich feed ingredient

Food and Chemical Toxicology (2020)

Gerald Patrick Dillon Jason D. Keegan Colm A. Moran

The purpose of this paper was to evaluate the toxicological potential of a heterotrophically grown unextracted Aurantiochytrium limacinum biomass (AURA) when used as a food additive. The following toxicological assessments were conducted on this novel docosahexaenoic acid rich feed ingredient: Mutagenic activity was tested by means of the Ames' test using five Salmonella typhimurium strains; clastogenic activity was investigated using the micronucleus test in male and female Sprague Dawley rats; genotoxic activity was assessed by means of the in vitro metaphase analysis tests in human lymphocytes; oral toxicity was tested by administration of AURA at various concentrations; eye and skin irritation was assessed in rabbits according to OECD guidelines; skin sensitivity was established in guinea pigs by means of the Buehler test. All conducted tests were considered valid under the experimental conditions. No significant mutagenic activity or clastogenic activity was observed. Genotoxic activity in human lymphocytes was not induced. Oral administration of 276 mg AURA/kg bw1 and 2000 mg AURA/kg bw resulted in no mortality or signs of acute toxicity. Daily administration of 1000 mg AURA/kg bw caused no mortality or biologically relevant signs of toxicity and was established as the No Observable Adverse Effect Level. AURA was also found to be a non-irritant for the eye and skin of the rabbit and was non-sensitizing to guinea pig skin.

Ames test

Clastogen

Ingredient

No-observed-adverse-effect level

10.1016/j.fct.2020.111397

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Citations (9)

Effect of feeding a commercial ammonia binding product De-Odorase™ on broiler chicken performance

Journal of Applied Animal Nutrition (2013)

Marina Lazarević Radmila Resanović Ivana Vučićević A. Kocher Colm A. Moran

Summary Ammonia production in commercial poultry sheds poses a problem for farmers as high levels can affect animal's respiratory health, resulting in hock and foot pad lesions and thereby affecting performance. A study was conducted to evaluate the suitability of whole, chopped and dried Yucca schidigera (De-Odorase™, Alltech Inc, USA) to reduce ammonia excretion from broilers and investigate the impact on broiler performance. Nine hundred and two, one-day-old Cobb broilers were randomly assigned to one of two diets, without (control) or supplemented with 500 g/t De-Odorase™. Birds fed the treatment diet had significantly higher body weight (P < 0.05) at 42 d and improved FCR (P < 0.05). Significant reductions in ammonia from litter were reported at 37 and 42 days old (P < 0.05 and P < 0.1, respectively). No significant differences were seen in foot pad, gait score, feather score or carcass data; although the birds fed De-Odorase™ had consistent numerical improvements in these parameters. The results of this study suggest that feeding whole, chopped and dried Yucca schidigera as a functional ingredient in the diet can be part of an ammonia management programme to improve broiler welfare and zootechnical performance.

10.1017/jan.2014.1

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Citations (8)